Microbial proteomics: a mass spectrometry primer for biologists

Robert Lj Graham, C Graham, Geoffrey McMullan

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    It is now more than 10 years since the publication of the first microbial genome sequence and science is now moving towards a post genomic era with transcriptomics and proteomics offering insights into cellular processes and function. The ability to assess the entire protein network of a cell at a given spatial or temporal point will have a profound effect upon microbial science as the function of proteins is inextricably linked to phenotype. Whilst such a situation is still beyond current technologies rapid advances in mass spectrometry, bioinformatics and protein separation technologies have produced a step change in our current proteomic capabilities. Subsequently a small, but steadily growing, number of groups are taking advantage of this cutting edge technology to discover more about the physiology and metabolism of microorganisms. From this research it will be possible to move towards a systems biology understanding of a microorganism. Where upon researchers can build a comprehensive cellular map for each microorganism that links an accurately annotated genome sequence to gene expression data, at a transcriptomic and proteomic level. In order for microbiologists to embrace the potential that proteomics offers, an understanding of a variety of analytical tools is required. The aim of this review is to provide a basic overview of mass spectrometry ( MS) and its application to protein identification. In addition we will describe how the protein complexity of microbial samples can be reduced by gel-based and gel-free methodologies prior to analysis by MS. Finally in order to illustrate the power of microbial proteomics a case study of its current application within the Bacilliaceae is given together with a description of the emerging discipline of metaproteomics.
    LanguageEnglish
    Pages26
    JournalMicrobial Cell Factories
    Volume6
    Publication statusPublished - Aug 2007

    Fingerprint

    Proteomics
    Mass Spectrometry
    Proteins
    Technology
    Gels
    Microbial Genome
    Systems Biology
    Computational Biology
    Publications
    Research Personnel
    Genome
    Phenotype
    Gene Expression
    Research

    Cite this

    Graham, R. L., Graham, C., & McMullan, G. (2007). Microbial proteomics: a mass spectrometry primer for biologists. Microbial Cell Factories, 6, 26.
    Graham, Robert Lj ; Graham, C ; McMullan, Geoffrey. / Microbial proteomics: a mass spectrometry primer for biologists. In: Microbial Cell Factories. 2007 ; Vol. 6. pp. 26.
    @article{4c9735965788468b85b4e461bf28d8aa,
    title = "Microbial proteomics: a mass spectrometry primer for biologists",
    abstract = "It is now more than 10 years since the publication of the first microbial genome sequence and science is now moving towards a post genomic era with transcriptomics and proteomics offering insights into cellular processes and function. The ability to assess the entire protein network of a cell at a given spatial or temporal point will have a profound effect upon microbial science as the function of proteins is inextricably linked to phenotype. Whilst such a situation is still beyond current technologies rapid advances in mass spectrometry, bioinformatics and protein separation technologies have produced a step change in our current proteomic capabilities. Subsequently a small, but steadily growing, number of groups are taking advantage of this cutting edge technology to discover more about the physiology and metabolism of microorganisms. From this research it will be possible to move towards a systems biology understanding of a microorganism. Where upon researchers can build a comprehensive cellular map for each microorganism that links an accurately annotated genome sequence to gene expression data, at a transcriptomic and proteomic level. In order for microbiologists to embrace the potential that proteomics offers, an understanding of a variety of analytical tools is required. The aim of this review is to provide a basic overview of mass spectrometry ( MS) and its application to protein identification. In addition we will describe how the protein complexity of microbial samples can be reduced by gel-based and gel-free methodologies prior to analysis by MS. Finally in order to illustrate the power of microbial proteomics a case study of its current application within the Bacilliaceae is given together with a description of the emerging discipline of metaproteomics.",
    author = "Graham, {Robert Lj} and C Graham and Geoffrey McMullan",
    year = "2007",
    month = "8",
    language = "English",
    volume = "6",
    pages = "26",
    journal = "Microbial Cell Factories",
    issn = "1475-2859",
    publisher = "BioMed Central",

    }

    Graham, RL, Graham, C & McMullan, G 2007, 'Microbial proteomics: a mass spectrometry primer for biologists', Microbial Cell Factories, vol. 6, pp. 26.

    Microbial proteomics: a mass spectrometry primer for biologists. / Graham, Robert Lj; Graham, C; McMullan, Geoffrey.

    In: Microbial Cell Factories, Vol. 6, 08.2007, p. 26.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Microbial proteomics: a mass spectrometry primer for biologists

    AU - Graham, Robert Lj

    AU - Graham, C

    AU - McMullan, Geoffrey

    PY - 2007/8

    Y1 - 2007/8

    N2 - It is now more than 10 years since the publication of the first microbial genome sequence and science is now moving towards a post genomic era with transcriptomics and proteomics offering insights into cellular processes and function. The ability to assess the entire protein network of a cell at a given spatial or temporal point will have a profound effect upon microbial science as the function of proteins is inextricably linked to phenotype. Whilst such a situation is still beyond current technologies rapid advances in mass spectrometry, bioinformatics and protein separation technologies have produced a step change in our current proteomic capabilities. Subsequently a small, but steadily growing, number of groups are taking advantage of this cutting edge technology to discover more about the physiology and metabolism of microorganisms. From this research it will be possible to move towards a systems biology understanding of a microorganism. Where upon researchers can build a comprehensive cellular map for each microorganism that links an accurately annotated genome sequence to gene expression data, at a transcriptomic and proteomic level. In order for microbiologists to embrace the potential that proteomics offers, an understanding of a variety of analytical tools is required. The aim of this review is to provide a basic overview of mass spectrometry ( MS) and its application to protein identification. In addition we will describe how the protein complexity of microbial samples can be reduced by gel-based and gel-free methodologies prior to analysis by MS. Finally in order to illustrate the power of microbial proteomics a case study of its current application within the Bacilliaceae is given together with a description of the emerging discipline of metaproteomics.

    AB - It is now more than 10 years since the publication of the first microbial genome sequence and science is now moving towards a post genomic era with transcriptomics and proteomics offering insights into cellular processes and function. The ability to assess the entire protein network of a cell at a given spatial or temporal point will have a profound effect upon microbial science as the function of proteins is inextricably linked to phenotype. Whilst such a situation is still beyond current technologies rapid advances in mass spectrometry, bioinformatics and protein separation technologies have produced a step change in our current proteomic capabilities. Subsequently a small, but steadily growing, number of groups are taking advantage of this cutting edge technology to discover more about the physiology and metabolism of microorganisms. From this research it will be possible to move towards a systems biology understanding of a microorganism. Where upon researchers can build a comprehensive cellular map for each microorganism that links an accurately annotated genome sequence to gene expression data, at a transcriptomic and proteomic level. In order for microbiologists to embrace the potential that proteomics offers, an understanding of a variety of analytical tools is required. The aim of this review is to provide a basic overview of mass spectrometry ( MS) and its application to protein identification. In addition we will describe how the protein complexity of microbial samples can be reduced by gel-based and gel-free methodologies prior to analysis by MS. Finally in order to illustrate the power of microbial proteomics a case study of its current application within the Bacilliaceae is given together with a description of the emerging discipline of metaproteomics.

    M3 - Article

    VL - 6

    SP - 26

    JO - Microbial Cell Factories

    T2 - Microbial Cell Factories

    JF - Microbial Cell Factories

    SN - 1475-2859

    ER -