Microbial decolorization and bioremediation of anaerobically digested molasses spent wash effluent by aerobic bacterial cultures

V Kumar, L Wati, Poonam Singh - Nee Nigam, IM Banat, G McMullan, D Singh, R Marchant

Research output: Contribution to journalArticle

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Abstract

Anaerobically digested molasses spent wash (DMSW) is a dark-brown-coloured recalcitrant effluent which has a high chemical oxygen demand (COD) and high pollution potential. Bacterial enrichment cultures were set up to obtain isolates capable of DMSW decolorization and bioremediation. Two aerobic bacterial cultures LA1 and D-2 were obtained using soil samples from an Indian distillery plant. Both grew well on 12.5% diluted digested spent wash supplemented with glucose as a readily available carbon source. The optimum conditions for growth and DMSW decolorization were 35 degrees C, pH 7.0, 3% glucose (w/v) and 0.5% peptone or yeast extract (w/v) in a basal mineral salt medium. The maximum decolorization (36.5% and 32.5%) and COD reduction (41.0% and 39.0%) were achieved for cultures LA1 and D-2, respectively, after 8 days incubation under the stated conditions. The advantages of using such a culture in digested spent wash bioremediation are discussed.
LanguageEnglish
Pages81-90
JournalMicrobios
Volume89
Issue number359
Publication statusPublished - 1997

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bioremediation
effluent
chemical oxygen demand
glucose
yeast
incubation
salt
pollution
carbon
mineral
soil

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title = "Microbial decolorization and bioremediation of anaerobically digested molasses spent wash effluent by aerobic bacterial cultures",
abstract = "Anaerobically digested molasses spent wash (DMSW) is a dark-brown-coloured recalcitrant effluent which has a high chemical oxygen demand (COD) and high pollution potential. Bacterial enrichment cultures were set up to obtain isolates capable of DMSW decolorization and bioremediation. Two aerobic bacterial cultures LA1 and D-2 were obtained using soil samples from an Indian distillery plant. Both grew well on 12.5{\%} diluted digested spent wash supplemented with glucose as a readily available carbon source. The optimum conditions for growth and DMSW decolorization were 35 degrees C, pH 7.0, 3{\%} glucose (w/v) and 0.5{\%} peptone or yeast extract (w/v) in a basal mineral salt medium. The maximum decolorization (36.5{\%} and 32.5{\%}) and COD reduction (41.0{\%} and 39.0{\%}) were achieved for cultures LA1 and D-2, respectively, after 8 days incubation under the stated conditions. The advantages of using such a culture in digested spent wash bioremediation are discussed.",
author = "V Kumar and L Wati and {Singh - Nee Nigam}, Poonam and IM Banat and G McMullan and D Singh and R Marchant",
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language = "English",
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Microbial decolorization and bioremediation of anaerobically digested molasses spent wash effluent by aerobic bacterial cultures. / Kumar, V; Wati, L; Singh - Nee Nigam, Poonam; Banat, IM; McMullan, G; Singh, D; Marchant, R.

Vol. 89, No. 359, 1997, p. 81-90.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microbial decolorization and bioremediation of anaerobically digested molasses spent wash effluent by aerobic bacterial cultures

AU - Kumar, V

AU - Wati, L

AU - Singh - Nee Nigam, Poonam

AU - Banat, IM

AU - McMullan, G

AU - Singh, D

AU - Marchant, R

PY - 1997

Y1 - 1997

N2 - Anaerobically digested molasses spent wash (DMSW) is a dark-brown-coloured recalcitrant effluent which has a high chemical oxygen demand (COD) and high pollution potential. Bacterial enrichment cultures were set up to obtain isolates capable of DMSW decolorization and bioremediation. Two aerobic bacterial cultures LA1 and D-2 were obtained using soil samples from an Indian distillery plant. Both grew well on 12.5% diluted digested spent wash supplemented with glucose as a readily available carbon source. The optimum conditions for growth and DMSW decolorization were 35 degrees C, pH 7.0, 3% glucose (w/v) and 0.5% peptone or yeast extract (w/v) in a basal mineral salt medium. The maximum decolorization (36.5% and 32.5%) and COD reduction (41.0% and 39.0%) were achieved for cultures LA1 and D-2, respectively, after 8 days incubation under the stated conditions. The advantages of using such a culture in digested spent wash bioremediation are discussed.

AB - Anaerobically digested molasses spent wash (DMSW) is a dark-brown-coloured recalcitrant effluent which has a high chemical oxygen demand (COD) and high pollution potential. Bacterial enrichment cultures were set up to obtain isolates capable of DMSW decolorization and bioremediation. Two aerobic bacterial cultures LA1 and D-2 were obtained using soil samples from an Indian distillery plant. Both grew well on 12.5% diluted digested spent wash supplemented with glucose as a readily available carbon source. The optimum conditions for growth and DMSW decolorization were 35 degrees C, pH 7.0, 3% glucose (w/v) and 0.5% peptone or yeast extract (w/v) in a basal mineral salt medium. The maximum decolorization (36.5% and 32.5%) and COD reduction (41.0% and 39.0%) were achieved for cultures LA1 and D-2, respectively, after 8 days incubation under the stated conditions. The advantages of using such a culture in digested spent wash bioremediation are discussed.

M3 - Article

VL - 89

SP - 81

EP - 90

IS - 359

ER -