METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY

G FLYNN; L MCHALE; AP MCHALE

doi:10.1016/0304-3835(94)90016-7

METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY

G FLYNN
, L MCHALE
, AP MCHALE

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64%. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.

Original language	English
Pages (from-to)	225-229
Journal	Cancer Letters
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(94)90016-7
Publication status	Published (in print/issue) - Jul 1994

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SDG 3 Good Health and Well-being

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@article{d561db77277b4be19709f571ad6ad98a,

title = "METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY",

abstract = "With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64\%. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.",

author = "G FLYNN and L MCHALE and AP MCHALE",

year = "1994",

month = jul,

doi = "10.1016/0304-3835(94)90016-7",

language = "English",

volume = "82",

pages = "225--229",

journal = "Cancer Letters",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

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T1 - METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY

AU - FLYNN, G

AU - MCHALE, L

AU - MCHALE, AP

PY - 1994/7

Y1 - 1994/7

N2 - With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64%. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.

AB - With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64%. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.

U2 - 10.1016/0304-3835(94)90016-7

DO - 10.1016/0304-3835(94)90016-7

M3 - Article

VL - 82

SP - 225

EP - 229

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY

Abstract

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