METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY

G FLYNN, L MCHALE, AP MCHALE

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Abstract

With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64%. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.
LanguageEnglish
Pages225-229
JournalCANCER LETTERS
Volume82
Issue number2
DOIs
Publication statusPublished - Jul 1994

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Methotrexate
Erythrocytes
Light
Hematoporphyrin Derivative
Neoplasms
Phototherapy
Electroporation
Photochemotherapy
HeLa Cells
Cell Survival
Therapeutics
Drug Therapy

Cite this

@article{d561db77277b4be19709f571ad6ad98a,
title = "METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY",
abstract = "With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64{\%}. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.",
author = "G FLYNN and L MCHALE and AP MCHALE",
year = "1994",
month = "7",
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language = "English",
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METHOTREXATE-LOADED, PHOTOSENSITIZED ERYTHROCYTES - A PHOTO-ACTIVATABLE CARRIER/DELIVERY SYSTEM FOR USE IN CANCER-THERAPY. / FLYNN, G; MCHALE, L; MCHALE, AP.

Vol. 82, No. 2, 07.1994, p. 225-229.

Research output: Contribution to journalArticle

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AB - With a view towards the design of a system incorporating both the use of chemotherapeutics and photodynamic therapy for use in cancer treatment modalities, erythrocytes have been loaded with methotrexate and subsequently photosensitized by exposure to hematoporphyrin derivative. Loading of methotrexate into erythrocytes has been optimized by examining variations in electroporation conditions. Maximum loading indices observed were in the region of 64%. In order to obtain rapid pre-defined release of chemotherapeutic from the system, the erythrocytes were photosensitized. Light-dependent release of methotrexate from the system was examined. In addition, studies measuring the cytotoxic effects of light-activated release from the system using Hela cells as a target, suggested that decreases in cell viability following exposure to light resulted from the combined effects of chemotherapy and photoradiation therapy. Potential applications and advantages associated with this novel system are discussed.

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