Methods for investigating biosurfactants and bioemulsifiers: a review

Surekha Satpute, Arun Banpurkar, Prashant Dhakephalkar, Ibrahim Banat, Balu Chopade

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Microorganisms produce biosurfactant (BS)/bioemulsifier (BE) with wide structural and functional diversity which consequently results in the adoption of different techniques to investigate these diverse amphiphilic molecules. This review aims to compile information on different microbial screening methods, surface active products extraction procedures, and analytical terminologies used in this field. Different methods for screening microbial culture broth or cell biomass for surface active compounds production are also presented and their possible advantages and disadvantages highlighted. In addition, the most common methods for purification, detection, and structure determination for a wide range of BS and BE are introduced. Simple techniques such as precipitation using acetone, ammonium sulphate, solvent extraction, ultrafiltration, ion exchange, dialysis, ultrafiltration, lyophilization, isoelectric focusing (IEF), and thin layer chromatography (TLC) are described. Other more elaborate techniques including high pressure liquid chromatography (HPLC), infra red (IR), gas chromatography-mass spectroscopy (GC-MS), nuclear magnetic resonance (NMR), and fast atom bombardment mass spectroscopy (FAB-MS), protein digestion and amino acid sequencing are also elucidated. Various experimental strategies including static light scattering and hydrodynamic characterization for micelles have been discussed. A combination of various analytical methods are often essential in this area of research and a numbers of trials and errors to isolate, purify and characterize various surface active agents are required. This review introduces the various methodologies that are indispensable for studying biosurfactants and bioemulsifiers.
LanguageEnglish
Pages127-144
JournalCritical Reviews in Biotechnology
Volume30
Issue number2
Publication statusPublished - Jun 2010

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Ultrafiltration
Screening
High pressure liquid chromatography
Thin layer chromatography
Dialysis
Ammonium Sulfate
Micelles
Solvent extraction
Terminology
Acetone
Surface-Active Agents
Gas chromatography
Microorganisms
Light scattering
Nuclear magnetic resonance spectroscopy
Purification
Ion exchange
Biomass
Hydrodynamics
Spectroscopy

Cite this

Satpute, S., Banpurkar, A., Dhakephalkar, P., Banat, I., & Chopade, B. (2010). Methods for investigating biosurfactants and bioemulsifiers: a review. Critical Reviews in Biotechnology, 30(2), 127-144.
Satpute, Surekha ; Banpurkar, Arun ; Dhakephalkar, Prashant ; Banat, Ibrahim ; Chopade, Balu. / Methods for investigating biosurfactants and bioemulsifiers: a review. In: Critical Reviews in Biotechnology. 2010 ; Vol. 30, No. 2. pp. 127-144.
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Satpute, S, Banpurkar, A, Dhakephalkar, P, Banat, I & Chopade, B 2010, 'Methods for investigating biosurfactants and bioemulsifiers: a review', Critical Reviews in Biotechnology, vol. 30, no. 2, pp. 127-144.

Methods for investigating biosurfactants and bioemulsifiers: a review. / Satpute, Surekha; Banpurkar, Arun; Dhakephalkar, Prashant; Banat, Ibrahim; Chopade, Balu.

In: Critical Reviews in Biotechnology, Vol. 30, No. 2, 06.2010, p. 127-144.

Research output: Contribution to journalArticle

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Satpute S, Banpurkar A, Dhakephalkar P, Banat I, Chopade B. Methods for investigating biosurfactants and bioemulsifiers: a review. Critical Reviews in Biotechnology. 2010 Jun;30(2):127-144.