Methanol Metabolism and Ammonia Assimilation in 4 Methylophilus Strains

EG Elrayes, Ibrahim Banat, IY Hamdan

Research output: Contribution to journalArticle

Abstract

Methanol assimilation and dissimilation pathways and ammonia assimilation pathway were investigated in four obligate methanol-utilizing bacteria through the detection of key enzymes. Both hexulose phosphate synthetase and hexulose phosphate isomerase, key enzymes of the ribulose monophosphate pathway (RMP) for methanol assimilation were detected whereas four key enzymes (hydroxy pyruvate reductase, isocitrate lyase, malyl-CoA-lyase and glyoxylate aminotransferase) that are characteristic of the serine assimilation pathway were absent. Key enzymes for the two methanol dissimilation pathways, the linear sequence enzymes formaldehyde and formate dehydrogenase and the RMP cyclic sequence enzymes glucose-6-phosphate dehydrogenase and 6-phosphate gluconate dehydrogenase were all detected. Ammonia was assimilated via the glutamate dehydrogenase pathway and not via the glutamine synthetase and glutamate synthase pathway.
LanguageEnglish
Pages87-93
JournalACTA Biotechnologica
Volume11
Issue number2
Publication statusPublished - 1991

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Methylophilus
Ammonia
Metabolism
Methanol
Enzymes
Phosphates
serine-glyoxylate aminotransferase
glutathione-independent formaldehyde dehydrogenase
phosphogluconate dehydrogenase (decarboxylating)
Glutamate Synthase
Formate Dehydrogenases
Isocitrate Lyase
Isomerases
Glutamate Dehydrogenase
Glutamate-Ammonia Ligase
Glucosephosphate Dehydrogenase
Ligases
Pyruvic Acid
Formaldehyde
Glucose

Cite this

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title = "Methanol Metabolism and Ammonia Assimilation in 4 Methylophilus Strains",
abstract = "Methanol assimilation and dissimilation pathways and ammonia assimilation pathway were investigated in four obligate methanol-utilizing bacteria through the detection of key enzymes. Both hexulose phosphate synthetase and hexulose phosphate isomerase, key enzymes of the ribulose monophosphate pathway (RMP) for methanol assimilation were detected whereas four key enzymes (hydroxy pyruvate reductase, isocitrate lyase, malyl-CoA-lyase and glyoxylate aminotransferase) that are characteristic of the serine assimilation pathway were absent. Key enzymes for the two methanol dissimilation pathways, the linear sequence enzymes formaldehyde and formate dehydrogenase and the RMP cyclic sequence enzymes glucose-6-phosphate dehydrogenase and 6-phosphate gluconate dehydrogenase were all detected. Ammonia was assimilated via the glutamate dehydrogenase pathway and not via the glutamine synthetase and glutamate synthase pathway.",
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Methanol Metabolism and Ammonia Assimilation in 4 Methylophilus Strains. / Elrayes, EG; Banat, Ibrahim; Hamdan, IY.

In: ACTA Biotechnologica, Vol. 11, No. 2, 1991, p. 87-93.

Research output: Contribution to journalArticle

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AU - Hamdan, IY

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AB - Methanol assimilation and dissimilation pathways and ammonia assimilation pathway were investigated in four obligate methanol-utilizing bacteria through the detection of key enzymes. Both hexulose phosphate synthetase and hexulose phosphate isomerase, key enzymes of the ribulose monophosphate pathway (RMP) for methanol assimilation were detected whereas four key enzymes (hydroxy pyruvate reductase, isocitrate lyase, malyl-CoA-lyase and glyoxylate aminotransferase) that are characteristic of the serine assimilation pathway were absent. Key enzymes for the two methanol dissimilation pathways, the linear sequence enzymes formaldehyde and formate dehydrogenase and the RMP cyclic sequence enzymes glucose-6-phosphate dehydrogenase and 6-phosphate gluconate dehydrogenase were all detected. Ammonia was assimilated via the glutamate dehydrogenase pathway and not via the glutamine synthetase and glutamate synthase pathway.

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