Metabolism of substance P in human plasma and in the rat circulation

J. Michael Conlon, Burkhard Göke

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17 Citations (Scopus)

Abstract

Incubation of substance P in human plasma at 37°C resulted in rapid conversion to des (Arg1Pro2) substance P (fragment 3-11) and to des (Arg1-Pro2-Lys3-Pro4) substance P (fragment 5-11). The metabolites were purified by high-performance liquid chromatography and identified by sequence analysis. These data are consistent with the hypothesis that substance P is metabolized by enzyme(s) with the specifity of dipeptidyl aminopeptidase IV (EC 3.1.14.5). Analysis by high-performance liquid chromatography of plasma extracts following intravenous infusion of substance P (300-350 nmoles) into anaesthetized rats showed that the peptide was cleared from the circulation within 1-2 minutes. No circulating metabolites could be identified.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalJOURNAL OF CHROMATOGRAPHY A
Volume296
Issue numberC
DOIs
Publication statusPublished (in print/issue) - 27 Jul 1984

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