Metabolic Interactions of Glucose, Acetoacetate and Insulin in Mammary- Glad Slices of Lactating Rats

DH Williamson, SR McKeown, V Ilic

    Research output: Contribution to journalArticle

    Abstract

    1.Utilisation of 5mM- glucose by slices of lactating mammary gland was decreased 33% on addition of acetoacetate (2mM) to the incubation medium. This inhabitation was accompanied by increases in the intracellular concentrations if citrate and glucose 6-phosphate. 2.In the presence of acetoacetate the accumulation of pyruvate in the medium approximately doubled. 3.Insulin completely reversed the inhibitory effect of acetoacetate on glucose utilisation, without altering the amount of acetoacetate removed or pyruvate formed. 4.Similar results were obtained with mammary -gland slices from diabetic rats, except that insulin did not completely reverse the effects of the acetoacetate. 5. Acetoacetate inhibited the formation of 14CO2 [1-14C]pryuvate; this effect was not overcome by insulin. 6. Insulin increased the proportion of [3-14C] acetoacetate that was converted into lipid and decreased that oxidized to CO2. 7. The physiological significance of these findings is discussed.
    LanguageEnglish
    Pages145-152
    JournalBiochemical Journal
    Volume150
    Issue number2
    Publication statusPublished - 1 Sep 1975

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    Rats
    Breast
    Insulin
    Glucose
    Human Mammary Glands
    Pyruvic Acid
    Glucose-6-Phosphate
    acetoacetic acid
    Citric Acid
    Lipids

    Cite this

    Williamson, DH ; McKeown, SR ; Ilic, V. / Metabolic Interactions of Glucose, Acetoacetate and Insulin in Mammary- Glad Slices of Lactating Rats. In: Biochemical Journal. 1975 ; Vol. 150, No. 2. pp. 145-152.
    @article{388694c25ab6458da907144178c55776,
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    abstract = "1.Utilisation of 5mM- glucose by slices of lactating mammary gland was decreased 33{\%} on addition of acetoacetate (2mM) to the incubation medium. This inhabitation was accompanied by increases in the intracellular concentrations if citrate and glucose 6-phosphate. 2.In the presence of acetoacetate the accumulation of pyruvate in the medium approximately doubled. 3.Insulin completely reversed the inhibitory effect of acetoacetate on glucose utilisation, without altering the amount of acetoacetate removed or pyruvate formed. 4.Similar results were obtained with mammary -gland slices from diabetic rats, except that insulin did not completely reverse the effects of the acetoacetate. 5. Acetoacetate inhibited the formation of 14CO2 [1-14C]pryuvate; this effect was not overcome by insulin. 6. Insulin increased the proportion of [3-14C] acetoacetate that was converted into lipid and decreased that oxidized to CO2. 7. The physiological significance of these findings is discussed.",
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    Metabolic Interactions of Glucose, Acetoacetate and Insulin in Mammary- Glad Slices of Lactating Rats. / Williamson, DH; McKeown, SR; Ilic, V.

    In: Biochemical Journal, Vol. 150, No. 2, 01.09.1975, p. 145-152.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Metabolic Interactions of Glucose, Acetoacetate and Insulin in Mammary- Glad Slices of Lactating Rats

    AU - Williamson, DH

    AU - McKeown, SR

    AU - Ilic, V

    PY - 1975/9/1

    Y1 - 1975/9/1

    N2 - 1.Utilisation of 5mM- glucose by slices of lactating mammary gland was decreased 33% on addition of acetoacetate (2mM) to the incubation medium. This inhabitation was accompanied by increases in the intracellular concentrations if citrate and glucose 6-phosphate. 2.In the presence of acetoacetate the accumulation of pyruvate in the medium approximately doubled. 3.Insulin completely reversed the inhibitory effect of acetoacetate on glucose utilisation, without altering the amount of acetoacetate removed or pyruvate formed. 4.Similar results were obtained with mammary -gland slices from diabetic rats, except that insulin did not completely reverse the effects of the acetoacetate. 5. Acetoacetate inhibited the formation of 14CO2 [1-14C]pryuvate; this effect was not overcome by insulin. 6. Insulin increased the proportion of [3-14C] acetoacetate that was converted into lipid and decreased that oxidized to CO2. 7. The physiological significance of these findings is discussed.

    AB - 1.Utilisation of 5mM- glucose by slices of lactating mammary gland was decreased 33% on addition of acetoacetate (2mM) to the incubation medium. This inhabitation was accompanied by increases in the intracellular concentrations if citrate and glucose 6-phosphate. 2.In the presence of acetoacetate the accumulation of pyruvate in the medium approximately doubled. 3.Insulin completely reversed the inhibitory effect of acetoacetate on glucose utilisation, without altering the amount of acetoacetate removed or pyruvate formed. 4.Similar results were obtained with mammary -gland slices from diabetic rats, except that insulin did not completely reverse the effects of the acetoacetate. 5. Acetoacetate inhibited the formation of 14CO2 [1-14C]pryuvate; this effect was not overcome by insulin. 6. Insulin increased the proportion of [3-14C] acetoacetate that was converted into lipid and decreased that oxidized to CO2. 7. The physiological significance of these findings is discussed.

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