Mechanisms underlying antidiabetic effects of Ocimum sanctum leaf extracts in animal models of type 1 and type 2 diabetes

JMA Hannan, Opeolu Ojo, L Ali, B Rokeya, J Khaleque, M Akhter, Peter Flatt, YHA Abdel-Wahab

Research output: Contribution to journalArticle

Abstract

Aim: This study investigated mechanisms by which O. sanctum leaf extracts ameliorate hyperglycaemia using animal and cellular models of diabetes. Place and Duration of Study: Diabetes Research Laboratory, University of Ulster, Coleraine, United Kingdom and Research Division, BIRDEM, Dhaka, Bangladesh; 8 months. Methodology: Acute anti-diabetic effects of ethanolic extracts of O. sanctum were examined in normal and chemically-induced type 1 and 2 diabetic rats. Effects of extracts on glucose absorption, intestinal disaccharidase activity and gastrointestinal motility in type 2 diabetic rats and on glucose uptake and insulin action in 3T3-L1 cells were assessed. Results: Treatment with the extract (1.25 g/kg bw) significantly improved oral glucose tolerance in normal and type 2 diabetic rats and suppressed blood glucose elevation after oral sucrose (2.5 g/kg bw) administration. The extract significantly reduced glucose absorption, gastrointestinal motility and disaccharidase activity. A 28-day treatment with O. sanctum decreased serum glucose, increased liver glycogen and enhanced circulating insulin and total oxidant status in type 2 diabetic rats. Glucose transport and insulin action in 3T3-L1 were increased by extract. Conclusion: O. sanctum represents a useful as a source for discovery of novel antidiabetic compounds and as a dietary adjunct for the management of type 2 diabetes and its complications
LanguageEnglish
Pages1-12
JournalEuropean Journal of Medicinal Plants
Volume5
Issue number1
DOIs
Publication statusPublished - 6 Sep 2014

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Type 1 Diabetes Mellitus
Hypoglycemic Agents
Type 2 Diabetes Mellitus
Animal Models
Glucose
Disaccharidases
Gastrointestinal Motility
Insulin
3T3-L1 Cells
Liver Glycogen
Bangladesh
Intestinal Absorption
Diabetes Complications
Glucose Tolerance Test
Research
Oxidants
Hyperglycemia
Sucrose
Blood Glucose
Ocimum sanctum

Keywords

  • Ocimum sanctum
  • 3T3 adipocytes
  • intestinal disaccharidase activity
  • hepatic glycogen
  • glucose uptake.

Cite this

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title = "Mechanisms underlying antidiabetic effects of Ocimum sanctum leaf extracts in animal models of type 1 and type 2 diabetes",
abstract = "Aim: This study investigated mechanisms by which O. sanctum leaf extracts ameliorate hyperglycaemia using animal and cellular models of diabetes. Place and Duration of Study: Diabetes Research Laboratory, University of Ulster, Coleraine, United Kingdom and Research Division, BIRDEM, Dhaka, Bangladesh; 8 months. Methodology: Acute anti-diabetic effects of ethanolic extracts of O. sanctum were examined in normal and chemically-induced type 1 and 2 diabetic rats. Effects of extracts on glucose absorption, intestinal disaccharidase activity and gastrointestinal motility in type 2 diabetic rats and on glucose uptake and insulin action in 3T3-L1 cells were assessed. Results: Treatment with the extract (1.25 g/kg bw) significantly improved oral glucose tolerance in normal and type 2 diabetic rats and suppressed blood glucose elevation after oral sucrose (2.5 g/kg bw) administration. The extract significantly reduced glucose absorption, gastrointestinal motility and disaccharidase activity. A 28-day treatment with O. sanctum decreased serum glucose, increased liver glycogen and enhanced circulating insulin and total oxidant status in type 2 diabetic rats. Glucose transport and insulin action in 3T3-L1 were increased by extract. Conclusion: O. sanctum represents a useful as a source for discovery of novel antidiabetic compounds and as a dietary adjunct for the management of type 2 diabetes and its complications",
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Mechanisms underlying antidiabetic effects of Ocimum sanctum leaf extracts in animal models of type 1 and type 2 diabetes. / Hannan, JMA; Ojo, Opeolu; Ali, L; Rokeya, B; Khaleque, J; Akhter, M; Flatt, Peter; Abdel-Wahab, YHA.

In: European Journal of Medicinal Plants, Vol. 5, No. 1, 06.09.2014, p. 1-12.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mechanisms underlying antidiabetic effects of Ocimum sanctum leaf extracts in animal models of type 1 and type 2 diabetes

AU - Hannan, JMA

AU - Ojo, Opeolu

AU - Ali, L

AU - Rokeya, B

AU - Khaleque, J

AU - Akhter, M

AU - Flatt, Peter

AU - Abdel-Wahab, YHA

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N2 - Aim: This study investigated mechanisms by which O. sanctum leaf extracts ameliorate hyperglycaemia using animal and cellular models of diabetes. Place and Duration of Study: Diabetes Research Laboratory, University of Ulster, Coleraine, United Kingdom and Research Division, BIRDEM, Dhaka, Bangladesh; 8 months. Methodology: Acute anti-diabetic effects of ethanolic extracts of O. sanctum were examined in normal and chemically-induced type 1 and 2 diabetic rats. Effects of extracts on glucose absorption, intestinal disaccharidase activity and gastrointestinal motility in type 2 diabetic rats and on glucose uptake and insulin action in 3T3-L1 cells were assessed. Results: Treatment with the extract (1.25 g/kg bw) significantly improved oral glucose tolerance in normal and type 2 diabetic rats and suppressed blood glucose elevation after oral sucrose (2.5 g/kg bw) administration. The extract significantly reduced glucose absorption, gastrointestinal motility and disaccharidase activity. A 28-day treatment with O. sanctum decreased serum glucose, increased liver glycogen and enhanced circulating insulin and total oxidant status in type 2 diabetic rats. Glucose transport and insulin action in 3T3-L1 were increased by extract. Conclusion: O. sanctum represents a useful as a source for discovery of novel antidiabetic compounds and as a dietary adjunct for the management of type 2 diabetes and its complications

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