Measurement of free cholesterol, cholesteryl esters and cholesteryl linoleate hydroperoxide in copper-oxidised low density lipoprotein in healthy volunteers supplemented with a low dose of n-3 polyunsaturated fatty acids

S Higgins, SN McCarthy, BM Corridan, HM Roche, Julie Wallace, NM O'Brien, PA Morrissey

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    Abstract

    A study was designed to evaluate the effect of supplementation with a low dose (0.9 g/d) of n-3 polyunsaturated fatty acids (n-3 PUFA) in fish oil on the oxidative modification of low-density lipoprotein (LDL) in a group of healthy volunteers. Eight volunteers were randomly selected from a larger fish oil supplementation study, and were required to take either 0.9g n-3 PUFA as fish oil (FO group) or 0.9g olive oil [control oil (CO group)] for 16 weeks. Oxidative modification of LDL was assessed by measuring concentrations of foe cholesterol (FC), cholesteryl esters (CE) and cholesteryl linoleate hydroperoxide (Ch18:2-OOH) in LDL following copper-induced lipid peroxidation for 0, 2, 3 and 4 h. The composition of LDL fatty acids over 4 h of copper-induced oxidation was also investigated. LDL eicosapentaenoic acid (C20:5n-3) and docosahexaenoic acid (C22:6n-3) compositions were significantly (P < 0.05) higher in the FO compared with the CO group, following supplementation. Linoleic acid (C18:2n-6), arachidonic acid (C20:4n-6), C20:5n-3 and C22:6n-3 were significantly (P < 0.05) oxidised in LDL following 4 h copper-oxidation. The proportions of palmitic acid (C16:0) (P < 0.05), palmitoleic acid (C16:1) (P < 0.05), stearic acid (C18:0), and oleic acid (C18:1) increased in the FO and CO groups, after 4 h of copper-oxidation. Concentrations of cholesteryl oleate (Ch18:1), cholesteryl linoleate (Ch18:2n-6), cholesteryl arachidonate (Ch20:4n-6) and cholesteryl docosahexanoate (Ch22:6n-3) were significantly (P < 0.05) reduced following copper-stimulated oxidation, in both groups. Ch18:2-OOH concentrations were significantly increased (P < 0.05) following 3 h oxidation in both groups compared with 0 h copper-oxidation, but decreased after 4 h. There was no significant difference in concentrations of Ch18:2-OOH between the groups over the time-course of copper-mediated oxidation. The results of this study suggest that moderate dietary intakes of n-3 PUFA do not significantly influence the susceptibility of LDL to copper-induced oxidation in vitro. (C) 2000 Elsevier Science Inc.
    LanguageEnglish
    Pages1091-1102
    JournalNutrition Research
    Volume20
    Issue number8
    Publication statusPublished - Aug 2000

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    cholesteryl esters
    low density lipoprotein
    hydroperoxides
    omega-3 fatty acids
    volunteers
    polyunsaturated fatty acids
    copper
    cholesterol
    oxidation
    dosage
    fish oils
    oleic acid
    palmitoleic acid
    eicosapentaenoic acid
    stearic acid
    docosahexaenoic acid
    arachidonic acid
    palmitic acid
    olive oil
    linoleic acid

    Cite this

    @article{abbbadcf24c84c039f31822fc6507474,
    title = "Measurement of free cholesterol, cholesteryl esters and cholesteryl linoleate hydroperoxide in copper-oxidised low density lipoprotein in healthy volunteers supplemented with a low dose of n-3 polyunsaturated fatty acids",
    abstract = "A study was designed to evaluate the effect of supplementation with a low dose (0.9 g/d) of n-3 polyunsaturated fatty acids (n-3 PUFA) in fish oil on the oxidative modification of low-density lipoprotein (LDL) in a group of healthy volunteers. Eight volunteers were randomly selected from a larger fish oil supplementation study, and were required to take either 0.9g n-3 PUFA as fish oil (FO group) or 0.9g olive oil [control oil (CO group)] for 16 weeks. Oxidative modification of LDL was assessed by measuring concentrations of foe cholesterol (FC), cholesteryl esters (CE) and cholesteryl linoleate hydroperoxide (Ch18:2-OOH) in LDL following copper-induced lipid peroxidation for 0, 2, 3 and 4 h. The composition of LDL fatty acids over 4 h of copper-induced oxidation was also investigated. LDL eicosapentaenoic acid (C20:5n-3) and docosahexaenoic acid (C22:6n-3) compositions were significantly (P < 0.05) higher in the FO compared with the CO group, following supplementation. Linoleic acid (C18:2n-6), arachidonic acid (C20:4n-6), C20:5n-3 and C22:6n-3 were significantly (P < 0.05) oxidised in LDL following 4 h copper-oxidation. The proportions of palmitic acid (C16:0) (P < 0.05), palmitoleic acid (C16:1) (P < 0.05), stearic acid (C18:0), and oleic acid (C18:1) increased in the FO and CO groups, after 4 h of copper-oxidation. Concentrations of cholesteryl oleate (Ch18:1), cholesteryl linoleate (Ch18:2n-6), cholesteryl arachidonate (Ch20:4n-6) and cholesteryl docosahexanoate (Ch22:6n-3) were significantly (P < 0.05) reduced following copper-stimulated oxidation, in both groups. Ch18:2-OOH concentrations were significantly increased (P < 0.05) following 3 h oxidation in both groups compared with 0 h copper-oxidation, but decreased after 4 h. There was no significant difference in concentrations of Ch18:2-OOH between the groups over the time-course of copper-mediated oxidation. The results of this study suggest that moderate dietary intakes of n-3 PUFA do not significantly influence the susceptibility of LDL to copper-induced oxidation in vitro. (C) 2000 Elsevier Science Inc.",
    author = "S Higgins and SN McCarthy and BM Corridan and HM Roche and Julie Wallace and NM O'Brien and PA Morrissey",
    year = "2000",
    month = "8",
    language = "English",
    volume = "20",
    pages = "1091--1102",
    number = "8",

    }

    Measurement of free cholesterol, cholesteryl esters and cholesteryl linoleate hydroperoxide in copper-oxidised low density lipoprotein in healthy volunteers supplemented with a low dose of n-3 polyunsaturated fatty acids. / Higgins, S; McCarthy, SN; Corridan, BM; Roche, HM; Wallace, Julie; O'Brien, NM; Morrissey, PA.

    Vol. 20, No. 8, 08.2000, p. 1091-1102.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Measurement of free cholesterol, cholesteryl esters and cholesteryl linoleate hydroperoxide in copper-oxidised low density lipoprotein in healthy volunteers supplemented with a low dose of n-3 polyunsaturated fatty acids

    AU - Higgins, S

    AU - McCarthy, SN

    AU - Corridan, BM

    AU - Roche, HM

    AU - Wallace, Julie

    AU - O'Brien, NM

    AU - Morrissey, PA

    PY - 2000/8

    Y1 - 2000/8

    N2 - A study was designed to evaluate the effect of supplementation with a low dose (0.9 g/d) of n-3 polyunsaturated fatty acids (n-3 PUFA) in fish oil on the oxidative modification of low-density lipoprotein (LDL) in a group of healthy volunteers. Eight volunteers were randomly selected from a larger fish oil supplementation study, and were required to take either 0.9g n-3 PUFA as fish oil (FO group) or 0.9g olive oil [control oil (CO group)] for 16 weeks. Oxidative modification of LDL was assessed by measuring concentrations of foe cholesterol (FC), cholesteryl esters (CE) and cholesteryl linoleate hydroperoxide (Ch18:2-OOH) in LDL following copper-induced lipid peroxidation for 0, 2, 3 and 4 h. The composition of LDL fatty acids over 4 h of copper-induced oxidation was also investigated. LDL eicosapentaenoic acid (C20:5n-3) and docosahexaenoic acid (C22:6n-3) compositions were significantly (P < 0.05) higher in the FO compared with the CO group, following supplementation. Linoleic acid (C18:2n-6), arachidonic acid (C20:4n-6), C20:5n-3 and C22:6n-3 were significantly (P < 0.05) oxidised in LDL following 4 h copper-oxidation. The proportions of palmitic acid (C16:0) (P < 0.05), palmitoleic acid (C16:1) (P < 0.05), stearic acid (C18:0), and oleic acid (C18:1) increased in the FO and CO groups, after 4 h of copper-oxidation. Concentrations of cholesteryl oleate (Ch18:1), cholesteryl linoleate (Ch18:2n-6), cholesteryl arachidonate (Ch20:4n-6) and cholesteryl docosahexanoate (Ch22:6n-3) were significantly (P < 0.05) reduced following copper-stimulated oxidation, in both groups. Ch18:2-OOH concentrations were significantly increased (P < 0.05) following 3 h oxidation in both groups compared with 0 h copper-oxidation, but decreased after 4 h. There was no significant difference in concentrations of Ch18:2-OOH between the groups over the time-course of copper-mediated oxidation. The results of this study suggest that moderate dietary intakes of n-3 PUFA do not significantly influence the susceptibility of LDL to copper-induced oxidation in vitro. (C) 2000 Elsevier Science Inc.

    AB - A study was designed to evaluate the effect of supplementation with a low dose (0.9 g/d) of n-3 polyunsaturated fatty acids (n-3 PUFA) in fish oil on the oxidative modification of low-density lipoprotein (LDL) in a group of healthy volunteers. Eight volunteers were randomly selected from a larger fish oil supplementation study, and were required to take either 0.9g n-3 PUFA as fish oil (FO group) or 0.9g olive oil [control oil (CO group)] for 16 weeks. Oxidative modification of LDL was assessed by measuring concentrations of foe cholesterol (FC), cholesteryl esters (CE) and cholesteryl linoleate hydroperoxide (Ch18:2-OOH) in LDL following copper-induced lipid peroxidation for 0, 2, 3 and 4 h. The composition of LDL fatty acids over 4 h of copper-induced oxidation was also investigated. LDL eicosapentaenoic acid (C20:5n-3) and docosahexaenoic acid (C22:6n-3) compositions were significantly (P < 0.05) higher in the FO compared with the CO group, following supplementation. Linoleic acid (C18:2n-6), arachidonic acid (C20:4n-6), C20:5n-3 and C22:6n-3 were significantly (P < 0.05) oxidised in LDL following 4 h copper-oxidation. The proportions of palmitic acid (C16:0) (P < 0.05), palmitoleic acid (C16:1) (P < 0.05), stearic acid (C18:0), and oleic acid (C18:1) increased in the FO and CO groups, after 4 h of copper-oxidation. Concentrations of cholesteryl oleate (Ch18:1), cholesteryl linoleate (Ch18:2n-6), cholesteryl arachidonate (Ch20:4n-6) and cholesteryl docosahexanoate (Ch22:6n-3) were significantly (P < 0.05) reduced following copper-stimulated oxidation, in both groups. Ch18:2-OOH concentrations were significantly increased (P < 0.05) following 3 h oxidation in both groups compared with 0 h copper-oxidation, but decreased after 4 h. There was no significant difference in concentrations of Ch18:2-OOH between the groups over the time-course of copper-mediated oxidation. The results of this study suggest that moderate dietary intakes of n-3 PUFA do not significantly influence the susceptibility of LDL to copper-induced oxidation in vitro. (C) 2000 Elsevier Science Inc.

    M3 - Article

    VL - 20

    SP - 1091

    EP - 1102

    IS - 8

    ER -