Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae)

Sara Moore, Franklin Smyth, Victor Gault, Edmund O'Kane, Stephen McClean

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8 Citations (Scopus)

Abstract

Arachnid venoms present a diverse and complex matrix for investigation, with their latent potential for innovative drug and pesticide design largely unrealised. The characterisation and quantification of selected low molecular mass compounds isolated from the crude venom of the Cobalt blue tarantula (Haplopelma lividum) were the objectives of this study. Fractionation of the crude venom was performed using reversed-phase high-performance liquid chromatography, with compound identification using both electrospray ionisation ion trap mass spectrometry and quadrupole time-of-flight mass spectrometry. Four compounds were identified, and quantification on a percentage dry weight basis was achieved by liquid chromatography/electrospray ionisation tandem mass spectrometry based on the formation of their corresponding product ions. Of these the most abundant component was glutamic acid, present at a level of 0.97%. Histamine and adenosine were detected at 0.14% and 0.10% dry weight, respectively, with the polyamine spermine noted in trace amounts at 0.002%. The limits of detection and quantification were established for each of the identified components. The fragmentation profile for histamine has also been proposed. Copyright (C) 2009 John Wiley & Sons, Ltd.
LanguageEnglish
Pages1747-1755
JournalRapid Communications in Mass Spectrometry
Volume23
Issue number12
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Venoms
Molecular mass
Mass spectrometry
Electrospray ionization
Histamine
Spider Venoms
Ions
Spermine
Liquid chromatography
Polyamines
High performance liquid chromatography
Fractionation
Cobalt
Pesticides
Adenosine
Glutamic Acid
Pharmaceutical Preparations

Cite this

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title = "Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae)",
abstract = "Arachnid venoms present a diverse and complex matrix for investigation, with their latent potential for innovative drug and pesticide design largely unrealised. The characterisation and quantification of selected low molecular mass compounds isolated from the crude venom of the Cobalt blue tarantula (Haplopelma lividum) were the objectives of this study. Fractionation of the crude venom was performed using reversed-phase high-performance liquid chromatography, with compound identification using both electrospray ionisation ion trap mass spectrometry and quadrupole time-of-flight mass spectrometry. Four compounds were identified, and quantification on a percentage dry weight basis was achieved by liquid chromatography/electrospray ionisation tandem mass spectrometry based on the formation of their corresponding product ions. Of these the most abundant component was glutamic acid, present at a level of 0.97{\%}. Histamine and adenosine were detected at 0.14{\%} and 0.10{\%} dry weight, respectively, with the polyamine spermine noted in trace amounts at 0.002{\%}. The limits of detection and quantification were established for each of the identified components. The fragmentation profile for histamine has also been proposed. Copyright (C) 2009 John Wiley & Sons, Ltd.",
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AU - Moore, Sara

AU - Smyth, Franklin

AU - Gault, Victor

AU - O'Kane, Edmund

AU - McClean, Stephen

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AB - Arachnid venoms present a diverse and complex matrix for investigation, with their latent potential for innovative drug and pesticide design largely unrealised. The characterisation and quantification of selected low molecular mass compounds isolated from the crude venom of the Cobalt blue tarantula (Haplopelma lividum) were the objectives of this study. Fractionation of the crude venom was performed using reversed-phase high-performance liquid chromatography, with compound identification using both electrospray ionisation ion trap mass spectrometry and quadrupole time-of-flight mass spectrometry. Four compounds were identified, and quantification on a percentage dry weight basis was achieved by liquid chromatography/electrospray ionisation tandem mass spectrometry based on the formation of their corresponding product ions. Of these the most abundant component was glutamic acid, present at a level of 0.97%. Histamine and adenosine were detected at 0.14% and 0.10% dry weight, respectively, with the polyamine spermine noted in trace amounts at 0.002%. The limits of detection and quantification were established for each of the identified components. The fragmentation profile for histamine has also been proposed. Copyright (C) 2009 John Wiley & Sons, Ltd.

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