Manuka Honey-induced Cytotoxicity against MCF7 Breast Cancer Cells is Correlated to Total Phenol Content and Antioxidant Power

I Portokalakis, HI Mohd Yusof, DF Ghanotakis, Poonam Singh Nigam, R.K. Owusu-Apenten

Research output: Contribution to journalArticle

Abstract

Aims: To investigate the relations between total polyphenols content, antioxidant power and Manuka honey cytotoxicity towards MCF-7 cells.Study Design: In vitro study.Place and Duration of Study: Department of Chemistry, University of Crete in partnership with the School of Biomedical Sciences, Ulster University, 09/ 2014 – 09/ 2015.Methodology: Manuka honey (UMF 5+,10+, 15+ and 18+) were examined for total phenols content using the Folin-Ciocalteu method with results expressed as mg gallic acid equivalents per kg honey(mg GAE/kg). Antioxidant power was evaluated using the Ferric Reducing Antioxidant Power“FRAP” method and expressed as mg GAE/kg. Honey cytotoxicity was examined with MCF-7 breast cancer cells cultured with RPMI 1640 supplemented with charcoals stripped serum and viability was monitored using the MTT assay.Results: The total phenols content for Manuka honey ranged from 1367±152 mg GAE/kg for UMF5+ honey to 2358 ±79 mg GAE/ kg for UMF 18+ honey. The antioxidant power for Manuka honey ranged from 170±22 mg GAE/kg for UMF 5+ honey rising to 266±21 mg GAE/kg for UMF 18+honey. Manuka honey showed dose-dependent cytotoxicity towards MCF-7 cells after 24 hrs.treatment. The concentration of honey which produces 50% inhibitory activity (IC50) ranged from4.7% (w/v) for UMF 5+ honey to 2.2% (w/v) for UMF 18+ honey. The cytotoxicity of Manuka honey was highly correlated with, values for the total phenols content (R2=0.99) and antioxidant power(R2=0.95) of Manuka Conclusion: Manuka honey is cytotoxic to MCF-7 breast cancer cells in vitro and the effects are correlated with the total phenols content and antioxidant power.
LanguageEnglish
Pages1-10
JournalJournal of Advances in Biology and Biotechnology
Volume8
Issue number2
DOIs
Publication statusPublished - 29 Jul 2016

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Honey
Phenol
Antioxidants
Breast Neoplasms
Gallic Acid
Phenols
MCF-7 Cells

Keywords

  • Manuka honey
  • MCF-7
  • antioxidant power
  • anticancer action
  • breast cancer
  • polyphenols

Cite this

Portokalakis, I ; Mohd Yusof, HI ; Ghanotakis, DF ; Singh Nigam, Poonam ; Owusu-Apenten, R.K. / Manuka Honey-induced Cytotoxicity against MCF7 Breast Cancer Cells is Correlated to Total Phenol Content and Antioxidant Power. 2016 ; Vol. 8, No. 2. pp. 1-10.
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abstract = "Aims: To investigate the relations between total polyphenols content, antioxidant power and Manuka honey cytotoxicity towards MCF-7 cells.Study Design: In vitro study.Place and Duration of Study: Department of Chemistry, University of Crete in partnership with the School of Biomedical Sciences, Ulster University, 09/ 2014 – 09/ 2015.Methodology: Manuka honey (UMF 5+,10+, 15+ and 18+) were examined for total phenols content using the Folin-Ciocalteu method with results expressed as mg gallic acid equivalents per kg honey(mg GAE/kg). Antioxidant power was evaluated using the Ferric Reducing Antioxidant Power“FRAP” method and expressed as mg GAE/kg. Honey cytotoxicity was examined with MCF-7 breast cancer cells cultured with RPMI 1640 supplemented with charcoals stripped serum and viability was monitored using the MTT assay.Results: The total phenols content for Manuka honey ranged from 1367±152 mg GAE/kg for UMF5+ honey to 2358 ±79 mg GAE/ kg for UMF 18+ honey. The antioxidant power for Manuka honey ranged from 170±22 mg GAE/kg for UMF 5+ honey rising to 266±21 mg GAE/kg for UMF 18+honey. Manuka honey showed dose-dependent cytotoxicity towards MCF-7 cells after 24 hrs.treatment. The concentration of honey which produces 50{\%} inhibitory activity (IC50) ranged from4.7{\%} (w/v) for UMF 5+ honey to 2.2{\%} (w/v) for UMF 18+ honey. The cytotoxicity of Manuka honey was highly correlated with, values for the total phenols content (R2=0.99) and antioxidant power(R2=0.95) of Manuka Conclusion: Manuka honey is cytotoxic to MCF-7 breast cancer cells in vitro and the effects are correlated with the total phenols content and antioxidant power.",
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Manuka Honey-induced Cytotoxicity against MCF7 Breast Cancer Cells is Correlated to Total Phenol Content and Antioxidant Power. / Portokalakis, I; Mohd Yusof, HI; Ghanotakis, DF; Singh Nigam, Poonam; Owusu-Apenten, R.K.

Vol. 8, No. 2, 29.07.2016, p. 1-10.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Portokalakis, I

AU - Mohd Yusof, HI

AU - Ghanotakis, DF

AU - Singh Nigam, Poonam

AU - Owusu-Apenten, R.K.

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N2 - Aims: To investigate the relations between total polyphenols content, antioxidant power and Manuka honey cytotoxicity towards MCF-7 cells.Study Design: In vitro study.Place and Duration of Study: Department of Chemistry, University of Crete in partnership with the School of Biomedical Sciences, Ulster University, 09/ 2014 – 09/ 2015.Methodology: Manuka honey (UMF 5+,10+, 15+ and 18+) were examined for total phenols content using the Folin-Ciocalteu method with results expressed as mg gallic acid equivalents per kg honey(mg GAE/kg). Antioxidant power was evaluated using the Ferric Reducing Antioxidant Power“FRAP” method and expressed as mg GAE/kg. Honey cytotoxicity was examined with MCF-7 breast cancer cells cultured with RPMI 1640 supplemented with charcoals stripped serum and viability was monitored using the MTT assay.Results: The total phenols content for Manuka honey ranged from 1367±152 mg GAE/kg for UMF5+ honey to 2358 ±79 mg GAE/ kg for UMF 18+ honey. The antioxidant power for Manuka honey ranged from 170±22 mg GAE/kg for UMF 5+ honey rising to 266±21 mg GAE/kg for UMF 18+honey. Manuka honey showed dose-dependent cytotoxicity towards MCF-7 cells after 24 hrs.treatment. The concentration of honey which produces 50% inhibitory activity (IC50) ranged from4.7% (w/v) for UMF 5+ honey to 2.2% (w/v) for UMF 18+ honey. The cytotoxicity of Manuka honey was highly correlated with, values for the total phenols content (R2=0.99) and antioxidant power(R2=0.95) of Manuka Conclusion: Manuka honey is cytotoxic to MCF-7 breast cancer cells in vitro and the effects are correlated with the total phenols content and antioxidant power.

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KW - Manuka honey

KW - MCF-7

KW - antioxidant power

KW - anticancer action

KW - breast cancer

KW - polyphenols

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DO - 10.9734/JABB/2016/27899

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