MALDI-TOF mass spectrometry misidentification of Cutibacterium namnetense and Cutibacterium modestum: Implications for multiplex PCR phylotyping of Cutibacterium acnes

Joseph McLaughlin; Carey-Ann D Burnham; Andrew McDowell

doi:10.1016/j.anaerobe.2024.102874

MALDI-TOF mass spectrometry misidentification of Cutibacterium namnetense and Cutibacterium modestum: Implications for multiplex PCR phylotyping of Cutibacterium acnes

Joseph McLaughlin
, Carey-Ann D Burnham
, Andrew McDowell

Research output: Contribution to journal › Article › peer-review

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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can misidentify Cutibacterium namnetense and Cutibacterium modestum as Cutibacterium acnes. We now describe how such MALDI-TOF MS misidentification explains previous reports of C. acnes isolates that could not be characterised using a multiplex PCR phylotyping assay.

Original language	English
Article number	102874
Pages (from-to)	1-4
Number of pages	4
Journal	Anaerobe
Volume	88
Early online date	6 Jun 2024
DOIs	https://doi.org/10.1016/j.anaerobe.2024.102874
Publication status	Published online - 6 Jun 2024

Bibliographical note

Data Access Statement

Data will be made available on request.

Funding

J.M. was funded by a British Skin Foundation studentship to A.M.D (Grant Project No. 025/s/16). We thank Dr Istvan Nagy, Institute of Biochemistry, Biological Research Centre, Szeged, Hungary, for conducting Sanger sequencing.

Funders	Funder number
British Skin Foundation	025/s/16

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Misidentification
Cutibacterium
Multiplex PCR phylotyping
MALDI-TOF MS

Access to Document

10.1016/j.anaerobe.2024.102874

1-s2.0-S107599642400057X-mainFinal published version, 1.23 MBLicence: CC BY

Cite this

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PY - 2024/6/6

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AB - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can misidentify Cutibacterium namnetense and Cutibacterium modestum as Cutibacterium acnes. We now describe how such MALDI-TOF MS misidentification explains previous reports of C. acnes isolates that could not be characterised using a multiplex PCR phylotyping assay.

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MALDI-TOF mass spectrometry misidentification of Cutibacterium namnetense and Cutibacterium modestum: Implications for multiplex PCR phylotyping of Cutibacterium acnes

Abstract

Bibliographical note

Data Access Statement

Funding

UN SDGs

Keywords

Access to Document

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Cite this