Abstract
Successful matrix-assisted laser desorption ionization
(MALDI) mass spectrometry imaging (MSI) relies on the selection of
the most appropriate matrix and optimization of the matrix application
parameters. In order to achieve reproducible high spatial-resolution
imaging data, several commercially available automated matrix application
platforms have become available. However, the high cost of these
commercial matrix sprayers is restricting access into this emerging research
field. Here, we report an automated platform for matrix deposition,
employing a converted commercially available 3D printer ($300) and other
parts commonly found in an analytical chemistry lab as a low-cost
alternative to commercial sprayers. Using printed fluorescent rhodamine B
microarrays and employing experimental design, the matrix deposition
parameters were optimized to minimize surface analyte diffusion. Finally,
the optimized matrix application method was applied to image three-dimensional MCF-7 cell culture spheroid sections (ca. 500
μm diameter tissue samples) and sections of mouse brain. Using this system, we demonstrate robust and reproducible
observations of endogenous metabolite and steroid distributions with a high spatial resolution.
(MALDI) mass spectrometry imaging (MSI) relies on the selection of
the most appropriate matrix and optimization of the matrix application
parameters. In order to achieve reproducible high spatial-resolution
imaging data, several commercially available automated matrix application
platforms have become available. However, the high cost of these
commercial matrix sprayers is restricting access into this emerging research
field. Here, we report an automated platform for matrix deposition,
employing a converted commercially available 3D printer ($300) and other
parts commonly found in an analytical chemistry lab as a low-cost
alternative to commercial sprayers. Using printed fluorescent rhodamine B
microarrays and employing experimental design, the matrix deposition
parameters were optimized to minimize surface analyte diffusion. Finally,
the optimized matrix application method was applied to image three-dimensional MCF-7 cell culture spheroid sections (ca. 500
μm diameter tissue samples) and sections of mouse brain. Using this system, we demonstrate robust and reproducible
observations of endogenous metabolite and steroid distributions with a high spatial resolution.
| Original language | English |
|---|---|
| Pages (from-to) | 8742-8749 |
| Number of pages | 8 |
| Journal | Analytical Chemistry |
| Volume | 90 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published (in print/issue) - 4 Jun 2018 |
