MALDI Matrix Application Utilizing a Modified 3D Printer for Accessible High Resolution Mass Spectrometry Imaging

Diego Cobice, Logan Mackay, Lulu Tucker, Antonio Conde-González, Colin Campbell, David Clarke, Goodwin Richard, Gregory Hamm

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Successful matrix-assisted laser desorption ionization
(MALDI) mass spectrometry imaging (MSI) relies on the selection of
the most appropriate matrix and optimization of the matrix application
parameters. In order to achieve reproducible high spatial-resolution
imaging data, several commercially available automated matrix application
platforms have become available. However, the high cost of these
commercial matrix sprayers is restricting access into this emerging research
field. Here, we report an automated platform for matrix deposition,
employing a converted commercially available 3D printer ($300) and other
parts commonly found in an analytical chemistry lab as a low-cost
alternative to commercial sprayers. Using printed fluorescent rhodamine B
microarrays and employing experimental design, the matrix deposition
parameters were optimized to minimize surface analyte diffusion. Finally,
the optimized matrix application method was applied to image three-dimensional MCF-7 cell culture spheroid sections (ca. 500
μm diameter tissue samples) and sections of mouse brain. Using this system, we demonstrate robust and reproducible
observations of endogenous metabolite and steroid distributions with a high spatial resolution.
LanguageEnglish
Pages8742-8749
Number of pages8
JournalAnalytical Chemistry
Volume90
Issue number15
DOIs
Publication statusPublished - 4 Jun 2018

Fingerprint

3D printers
Ionization
Mass spectrometry
Desorption
Imaging techniques
Lasers
Rhodamines
Surface diffusion
Metabolites
Cell culture
Design of experiments
Brain
Steroids
Tissue

Cite this

Cobice, Diego ; Mackay, Logan ; Tucker, Lulu ; Conde-González, Antonio ; Campbell, Colin ; Clarke, David ; Richard, Goodwin ; Hamm, Gregory. / MALDI Matrix Application Utilizing a Modified 3D Printer for Accessible High Resolution Mass Spectrometry Imaging. In: Analytical Chemistry. 2018 ; Vol. 90, No. 15. pp. 8742-8749.
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abstract = "Successful matrix-assisted laser desorption ionization(MALDI) mass spectrometry imaging (MSI) relies on the selection ofthe most appropriate matrix and optimization of the matrix applicationparameters. In order to achieve reproducible high spatial-resolutionimaging data, several commercially available automated matrix applicationplatforms have become available. However, the high cost of thesecommercial matrix sprayers is restricting access into this emerging researchfield. Here, we report an automated platform for matrix deposition,employing a converted commercially available 3D printer ($300) and otherparts commonly found in an analytical chemistry lab as a low-costalternative to commercial sprayers. Using printed fluorescent rhodamine Bmicroarrays and employing experimental design, the matrix depositionparameters were optimized to minimize surface analyte diffusion. Finally,the optimized matrix application method was applied to image three-dimensional MCF-7 cell culture spheroid sections (ca. 500μm diameter tissue samples) and sections of mouse brain. Using this system, we demonstrate robust and reproducibleobservations of endogenous metabolite and steroid distributions with a high spatial resolution.",
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Cobice, D, Mackay, L, Tucker, L, Conde-González, A, Campbell, C, Clarke, D, Richard, G & Hamm, G 2018, 'MALDI Matrix Application Utilizing a Modified 3D Printer for Accessible High Resolution Mass Spectrometry Imaging', Analytical Chemistry, vol. 90, no. 15, pp. 8742-8749. https://doi.org/10.1021/acs.analchem.8b00670

MALDI Matrix Application Utilizing a Modified 3D Printer for Accessible High Resolution Mass Spectrometry Imaging. / Cobice, Diego; Mackay, Logan; Tucker, Lulu ; Conde-González, Antonio ; Campbell, Colin ; Clarke, David ; Richard, Goodwin; Hamm, Gregory.

In: Analytical Chemistry, Vol. 90, No. 15, 04.06.2018, p. 8742-8749.

Research output: Contribution to journalArticle

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AU - Cobice, Diego

AU - Mackay, Logan

AU - Tucker, Lulu

AU - Conde-González, Antonio

AU - Campbell, Colin

AU - Clarke, David

AU - Richard, Goodwin

AU - Hamm, Gregory

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AB - Successful matrix-assisted laser desorption ionization(MALDI) mass spectrometry imaging (MSI) relies on the selection ofthe most appropriate matrix and optimization of the matrix applicationparameters. In order to achieve reproducible high spatial-resolutionimaging data, several commercially available automated matrix applicationplatforms have become available. However, the high cost of thesecommercial matrix sprayers is restricting access into this emerging researchfield. Here, we report an automated platform for matrix deposition,employing a converted commercially available 3D printer ($300) and otherparts commonly found in an analytical chemistry lab as a low-costalternative to commercial sprayers. Using printed fluorescent rhodamine Bmicroarrays and employing experimental design, the matrix depositionparameters were optimized to minimize surface analyte diffusion. Finally,the optimized matrix application method was applied to image three-dimensional MCF-7 cell culture spheroid sections (ca. 500μm diameter tissue samples) and sections of mouse brain. Using this system, we demonstrate robust and reproducibleobservations of endogenous metabolite and steroid distributions with a high spatial resolution.

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