Magainin-related peptides stimulate insulin-release and improve glucose tolerance in high fat fed mice

Opeolu Ojo, DK Srinivasan, BO Owolabi, Peter Flatt, Yasser Abdel-Wahab

Research output: Contribution to journalArticle

Abstract

Earlier peptidomic analysis of the skin secretion of Xenopus amieti led to the identification of orthologs of magainins and other peptides. This study investigated the degradation, in vitro insulin-releasing and acute metabolic effects of magainin-AM1 (GIKEFAHSLGKFG KAFVGGILNQ) and magainin-AM2 (GVSKILHSAGKFGKAFLGEIMKS). Plasma degradation was investigated using reversed-phase HPLC and MALDI-TOF mass spectroscopy. Insulin-releasing effects were determined using BRIN-BD11 clonal beta cells and mouse islets. Effects of magainin peptides on cytosolic enzyme lactate dehydrogenase release, membrane potential and intracellular Ca(2+) concentration were assessed using BRIN-BD11 cells while their in vivo effects on glucose tolerance and insulin release were assessed in obese, insulin-resistant Swiss National Institute of Health (NIH) mice. Both peptides were resistant to degradation by plasma enzymes in vitro for up to 8 h. Though magainin-AM1 elicited non-toxic, concentration-dependent stimulation of insulin-release from clonal BRINBD11 cells at concentrations ≥ 100nM, magainin-AM2 produced a higher stimulation of insulin-release from BRIN-BD11 cells and isolated mouse islets. Membrane depolarization and intracellular [Ca(2+)]i in BRIN-BD11 cells were significantly (P
LanguageEnglish
Pages256-263
JournalProtein and Peptide Letters
Volume22
Issue number3
Publication statusPublished - 1 Feb 2014

Fingerprint

Magainins
Fats
Insulin
Glucose
Peptides
Intracellular Membranes
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
National Institutes of Health (U.S.)
Enzymes
Xenopus
Islets of Langerhans
L-Lactate Dehydrogenase
Membrane Potentials
Mass Spectrometry
High Pressure Liquid Chromatography
Skin

Keywords

  • Cytokine
  • Toxicity
  • 1.1B4
  • Insulin secretion

Cite this

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abstract = "Earlier peptidomic analysis of the skin secretion of Xenopus amieti led to the identification of orthologs of magainins and other peptides. This study investigated the degradation, in vitro insulin-releasing and acute metabolic effects of magainin-AM1 (GIKEFAHSLGKFG KAFVGGILNQ) and magainin-AM2 (GVSKILHSAGKFGKAFLGEIMKS). Plasma degradation was investigated using reversed-phase HPLC and MALDI-TOF mass spectroscopy. Insulin-releasing effects were determined using BRIN-BD11 clonal beta cells and mouse islets. Effects of magainin peptides on cytosolic enzyme lactate dehydrogenase release, membrane potential and intracellular Ca(2+) concentration were assessed using BRIN-BD11 cells while their in vivo effects on glucose tolerance and insulin release were assessed in obese, insulin-resistant Swiss National Institute of Health (NIH) mice. Both peptides were resistant to degradation by plasma enzymes in vitro for up to 8 h. Though magainin-AM1 elicited non-toxic, concentration-dependent stimulation of insulin-release from clonal BRINBD11 cells at concentrations ≥ 100nM, magainin-AM2 produced a higher stimulation of insulin-release from BRIN-BD11 cells and isolated mouse islets. Membrane depolarization and intracellular [Ca(2+)]i in BRIN-BD11 cells were significantly (P",
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Magainin-related peptides stimulate insulin-release and improve glucose tolerance in high fat fed mice. / Ojo, Opeolu; Srinivasan, DK; Owolabi, BO; Flatt, Peter; Abdel-Wahab, Yasser.

In: Protein and Peptide Letters, Vol. 22, No. 3, 01.02.2014, p. 256-263.

Research output: Contribution to journalArticle

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AU - Ojo, Opeolu

AU - Srinivasan, DK

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AU - Flatt, Peter

AU - Abdel-Wahab, Yasser

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