Maerua angolensis extract reduces allodynia and hyperalgesia in a mouse model of vincristine-induced peripheral neuropathy

Allodynia and hyperalgesia comprise the main and frequent symptoms suffered by patients with neuropathic
pain, which responds poorly to therapy. An earlier study reported that stem bark extracts of Maerua angolensis
exhibited dose-dependent anti-nociceptive effect against the neurogenic and inflammatory phases of the
formalin test. The current study evaluated the effect of petroleum ether/ethyl acetate stem bark extract of
Maerua angolensis on vincristine-induced neuropathy. Neuropathic pain was induced by intraperitoneal
injection of vincristine (0.1 mg/kg/day) using a 5-day-on, 2-day-off schedule over 12 days. On day 15, baseline
responses were measured in the Randall-Selitto mechanical hyperalgesia test and paw withdrawal tests (using
Von Frey filaments and cold water at 4.5 °C) and mice that developed allodynia/hyperalgesia were randomly
assigned into 7 groups. Normal saline (i.p.), pregabalin (10, 30, and 100 mg/kg, p.o.) and extract (3, 10, and 20
mg/kg, p.o.) were administered to the individual groups. Allodynia/hyperalgesia was measured hourly for 5
hours post treatment. The extract produced significant (P<0.05) and dose-dependent inhibition of vincristineinduced mechanical hyperalgesia, tactile and cold allodynia responses. In all, the study shows that oral
administration of Maerua angolensis stem bark extract inhibits vincristine-induced neuropathy in mice
suggesting that it may exert analgesic effect in cancer patients with vincristine-induced neuropathic pain.