The cytochrome P450 system plays a key role in the metabolism of endogenous and exogenous compounds. The system is widely distributed in body tissues with the highest concentration of the enzymes found in the liver hepatocyte. Extra hepatic expression of the P450 system has been documented in the lung, pancreas and kidney and the enzymes have been shown to be induced by many disease states, including diabetes mellitus and cancer. Little attention has been paid to the expression and inducibility of the system in peripheral blood lymphocytes. In this present investigation, specific P450 inducers were administered in vivo to male Wistar rats. The expression and in vivo induction of the P450 isoforms; CYP2B, CYP2E, CYP3A and CYP4A, within liver and lymphocyte samples was determined using Western blot analysis. Following in vivo induction the lymphocyte P450 proteins showed an average 3-fold increase in expression (0.003-0.005 g P450/g microsomal protein), compared to the control lymphocyte samples. Expression in the induced lymphocyte samples was up to 11-fold lower than that of the induced liver samples, as expected. Results indicate that lymphocytes may provide a relatively simple method for monitoring the P450 profile in human subjects.
|Journal||British Journal of Biomedical Science|
|Publication status||Published - 2008|