Luminescent Detection of ATP in Aqueous Solution Using Positively Charged CdSe-ZnS Quantum Dots

John F. Callan, Ray C. Mulrooney, Sukanta Karnila

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    Commercially available CdSe-ZnS Quantum Dots (QDs) have been modified by exchanging the hydrophobic surface ligands with (2-mercaptoethyl)-trimethylammonium chloride. The resulting water soluble conjugate was titrated with solutions of adenosine triphosphate (ATP), adenosine diphosphate, adenosine monophosphate, guanosine triphosphate (GTP), guanosine diphosphate and guanosine monophosphate in 0.01 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.4). A strong fluorescence quench of about 80% was observed for ATP, a quench of 25% was observed for GTP while the others had virtually no effect. The quenching effect of ATP and GTP was attributed to the high negative charge density associated with these substrate's resulting in a strong attraction to the QD surface enabling them to engage in electron transfer with the excited QD. The lack of fluorescence quenching associated with the other nucleotides was most likely due to their reduced charge density resulting in a lower affinity for the QD surface.
    LanguageEnglish
    Pages1157-1161
    JournalJournal of Fluorescence
    Volume18
    Issue number6
    DOIs
    Publication statusPublished - Nov 2008

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    Semiconductor quantum dots
    Adenosine Triphosphate
    Guanosine Triphosphate
    Charge density
    Quenching
    Fluorescence
    Guanosine Monophosphate
    HEPES
    Guanosine
    Diphosphates
    Chlorides
    Buffers
    Nucleotides
    Ligands
    Electrons
    Water
    Substrates

    Cite this

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    title = "Luminescent Detection of ATP in Aqueous Solution Using Positively Charged CdSe-ZnS Quantum Dots",
    abstract = "Commercially available CdSe-ZnS Quantum Dots (QDs) have been modified by exchanging the hydrophobic surface ligands with (2-mercaptoethyl)-trimethylammonium chloride. The resulting water soluble conjugate was titrated with solutions of adenosine triphosphate (ATP), adenosine diphosphate, adenosine monophosphate, guanosine triphosphate (GTP), guanosine diphosphate and guanosine monophosphate in 0.01 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.4). A strong fluorescence quench of about 80{\%} was observed for ATP, a quench of 25{\%} was observed for GTP while the others had virtually no effect. The quenching effect of ATP and GTP was attributed to the high negative charge density associated with these substrate's resulting in a strong attraction to the QD surface enabling them to engage in electron transfer with the excited QD. The lack of fluorescence quenching associated with the other nucleotides was most likely due to their reduced charge density resulting in a lower affinity for the QD surface.",
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    language = "English",
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    Luminescent Detection of ATP in Aqueous Solution Using Positively Charged CdSe-ZnS Quantum Dots. / Callan, John F.; Mulrooney, Ray C.; Karnila, Sukanta.

    In: Journal of Fluorescence, Vol. 18, No. 6, 11.2008, p. 1157-1161.

    Research output: Contribution to journalArticle

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    AU - Mulrooney, Ray C.

    AU - Karnila, Sukanta

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    AB - Commercially available CdSe-ZnS Quantum Dots (QDs) have been modified by exchanging the hydrophobic surface ligands with (2-mercaptoethyl)-trimethylammonium chloride. The resulting water soluble conjugate was titrated with solutions of adenosine triphosphate (ATP), adenosine diphosphate, adenosine monophosphate, guanosine triphosphate (GTP), guanosine diphosphate and guanosine monophosphate in 0.01 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.4). A strong fluorescence quench of about 80% was observed for ATP, a quench of 25% was observed for GTP while the others had virtually no effect. The quenching effect of ATP and GTP was attributed to the high negative charge density associated with these substrate's resulting in a strong attraction to the QD surface enabling them to engage in electron transfer with the excited QD. The lack of fluorescence quenching associated with the other nucleotides was most likely due to their reduced charge density resulting in a lower affinity for the QD surface.

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