Lower Cathelicidin Concentrations In Irish Athletes Compared To Healthy Controls: A Role For Vitamin D?

Joshua J Todd, Emeir M McSorley, L. Kirsty Pourshahidi, Sharon M Madigan, Eamon L Laird, Ruth R Weir, Martin Healy, Pamela J Magee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The biologically active vitamin D metabolite (1[alpha]-dihydroxyvitamin D) has been linked to regulation of human cathelicidin antimicrobial peptide (LL-37) in vitro. Recent studies in athletes indicate that vitamin D inadequacy is associated with lower concentrations of LL-37 and that this may increase risk of upper respiratory tract infection (URTI). PURPOSE: To compare circulating LL-37 concentrations between athletes and healthy controls and to establish if total 25-hydroxyvitamin D (25[OH]D) was a significant determinant of this antimicrobial peptide.METHODS: A total of 221 stored, fasted plasma samples from male and female athletes (elite rugby n=12, elite boxing n=12, Gaelic football n=77) and age and sex-matched healthy controls (n=120) were analysed in duplicate for LL-37 concentrations using an enzyme-linked immunosorbent assay. Total 25(OH)D concentrations were quantified by liquid chromatography-tandem mass spectrometry as part of the previous studies. Comparison of 25(OH)D and LL-37 concentrations between athletes and controls was made using Mann-Whitney U tests. Linear regression was used to test if 25(OH)D concentration was a significant determinant of LL-37 concentration after adjusting for season of sampling.RESULTS: Median [IQR] 25(OH)D concentrations were 49.76[39.37] and 28.90[23.55] nmol/L for athletes and healthy controls respectively, P
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages341
Number of pages1
Volume48
DOIs
Publication statusE-pub ahead of print - 31 May 2016
EventAmerican College of Sports Medicine (ACSM) Annual Meeting 2016 - Boston, Massachusetts
Duration: 31 May 2016 → …

Conference

ConferenceAmerican College of Sports Medicine (ACSM) Annual Meeting 2016
Period31/05/16 → …

Fingerprint

Vitamin D
Athletes
Football
Boxing
Dihydroxycholecalciferols
Nonparametric Statistics
Tandem Mass Spectrometry
Liquid Chromatography
Respiratory Tract Infections
Linear Models
Enzyme-Linked Immunosorbent Assay
CAP18 lipopolysaccharide-binding protein
Peptides

Keywords

  • Vitamin D
  • Cathelicidin

Cite this

Todd, Joshua J ; McSorley, Emeir M ; Pourshahidi, L. Kirsty ; Madigan, Sharon M ; Laird, Eamon L ; Weir, Ruth R ; Healy, Martin ; Magee, Pamela J. / Lower Cathelicidin Concentrations In Irish Athletes Compared To Healthy Controls: A Role For Vitamin D?. Unknown Host Publication. Vol. 48 2016. pp. 341
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title = "Lower Cathelicidin Concentrations In Irish Athletes Compared To Healthy Controls: A Role For Vitamin D?",
abstract = "The biologically active vitamin D metabolite (1[alpha]-dihydroxyvitamin D) has been linked to regulation of human cathelicidin antimicrobial peptide (LL-37) in vitro. Recent studies in athletes indicate that vitamin D inadequacy is associated with lower concentrations of LL-37 and that this may increase risk of upper respiratory tract infection (URTI). PURPOSE: To compare circulating LL-37 concentrations between athletes and healthy controls and to establish if total 25-hydroxyvitamin D (25[OH]D) was a significant determinant of this antimicrobial peptide.METHODS: A total of 221 stored, fasted plasma samples from male and female athletes (elite rugby n=12, elite boxing n=12, Gaelic football n=77) and age and sex-matched healthy controls (n=120) were analysed in duplicate for LL-37 concentrations using an enzyme-linked immunosorbent assay. Total 25(OH)D concentrations were quantified by liquid chromatography-tandem mass spectrometry as part of the previous studies. Comparison of 25(OH)D and LL-37 concentrations between athletes and controls was made using Mann-Whitney U tests. Linear regression was used to test if 25(OH)D concentration was a significant determinant of LL-37 concentration after adjusting for season of sampling.RESULTS: Median [IQR] 25(OH)D concentrations were 49.76[39.37] and 28.90[23.55] nmol/L for athletes and healthy controls respectively, P",
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Todd, JJ, McSorley, EM, Pourshahidi, LK, Madigan, SM, Laird, EL, Weir, RR, Healy, M & Magee, PJ 2016, Lower Cathelicidin Concentrations In Irish Athletes Compared To Healthy Controls: A Role For Vitamin D? in Unknown Host Publication. vol. 48, pp. 341, American College of Sports Medicine (ACSM) Annual Meeting 2016, 31/05/16. https://doi.org/10.1249/01.mss.0000486035.92181.14

Lower Cathelicidin Concentrations In Irish Athletes Compared To Healthy Controls: A Role For Vitamin D? / Todd, Joshua J; McSorley, Emeir M; Pourshahidi, L. Kirsty; Madigan, Sharon M; Laird, Eamon L; Weir, Ruth R; Healy, Martin; Magee, Pamela J.

Unknown Host Publication. Vol. 48 2016. p. 341.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Todd, Joshua J

AU - McSorley, Emeir M

AU - Pourshahidi, L. Kirsty

AU - Madigan, Sharon M

AU - Laird, Eamon L

AU - Weir, Ruth R

AU - Healy, Martin

AU - Magee, Pamela J

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N2 - The biologically active vitamin D metabolite (1[alpha]-dihydroxyvitamin D) has been linked to regulation of human cathelicidin antimicrobial peptide (LL-37) in vitro. Recent studies in athletes indicate that vitamin D inadequacy is associated with lower concentrations of LL-37 and that this may increase risk of upper respiratory tract infection (URTI). PURPOSE: To compare circulating LL-37 concentrations between athletes and healthy controls and to establish if total 25-hydroxyvitamin D (25[OH]D) was a significant determinant of this antimicrobial peptide.METHODS: A total of 221 stored, fasted plasma samples from male and female athletes (elite rugby n=12, elite boxing n=12, Gaelic football n=77) and age and sex-matched healthy controls (n=120) were analysed in duplicate for LL-37 concentrations using an enzyme-linked immunosorbent assay. Total 25(OH)D concentrations were quantified by liquid chromatography-tandem mass spectrometry as part of the previous studies. Comparison of 25(OH)D and LL-37 concentrations between athletes and controls was made using Mann-Whitney U tests. Linear regression was used to test if 25(OH)D concentration was a significant determinant of LL-37 concentration after adjusting for season of sampling.RESULTS: Median [IQR] 25(OH)D concentrations were 49.76[39.37] and 28.90[23.55] nmol/L for athletes and healthy controls respectively, P

AB - The biologically active vitamin D metabolite (1[alpha]-dihydroxyvitamin D) has been linked to regulation of human cathelicidin antimicrobial peptide (LL-37) in vitro. Recent studies in athletes indicate that vitamin D inadequacy is associated with lower concentrations of LL-37 and that this may increase risk of upper respiratory tract infection (URTI). PURPOSE: To compare circulating LL-37 concentrations between athletes and healthy controls and to establish if total 25-hydroxyvitamin D (25[OH]D) was a significant determinant of this antimicrobial peptide.METHODS: A total of 221 stored, fasted plasma samples from male and female athletes (elite rugby n=12, elite boxing n=12, Gaelic football n=77) and age and sex-matched healthy controls (n=120) were analysed in duplicate for LL-37 concentrations using an enzyme-linked immunosorbent assay. Total 25(OH)D concentrations were quantified by liquid chromatography-tandem mass spectrometry as part of the previous studies. Comparison of 25(OH)D and LL-37 concentrations between athletes and controls was made using Mann-Whitney U tests. Linear regression was used to test if 25(OH)D concentration was a significant determinant of LL-37 concentration after adjusting for season of sampling.RESULTS: Median [IQR] 25(OH)D concentrations were 49.76[39.37] and 28.90[23.55] nmol/L for athletes and healthy controls respectively, P

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