Low temperature organic phase biocatalysis using cold-adapted lipase from psychrotrophic Pseudomonas P38

Sze Tan, Richard Owusu-Apenten, JS Knapp

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Lipase produced by a psychrotroph, Pseudomonas fluorescens P38, was found to catalyse the synthesis of butyl caprylate in n-heptane at low temperatures. The optimum yield of ester synthesis was 75% at 20 °C with an organic phase water concentration of 0.25% (v/v). The results are discussed in terms of the structural flexibility of psychrotroph-derived lipase and the activity of this enzyme within a nearly anhydrous organic solvent phase.
Original languageEnglish
Pages (from-to)415-418
JournalFood Chemistry
Volume57
Issue number3
DOIs
Publication statusPublished - Nov 1996

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