Low temperature organic phase biocatalysis using cold-adapted lipase from psychrotrophic Pseudomonas P38

Sze Tan, Richard Owusu-Apenten, JS Knapp

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Lipase produced by a psychrotroph, Pseudomonas fluorescens P38, was found to catalyse the synthesis of butyl caprylate in n-heptane at low temperatures. The optimum yield of ester synthesis was 75% at 20 °C with an organic phase water concentration of 0.25% (v/v). The results are discussed in terms of the structural flexibility of psychrotroph-derived lipase and the activity of this enzyme within a nearly anhydrous organic solvent phase.
LanguageEnglish
Pages415-418
JournalFood Chemistry
Volume57
Issue number3
DOIs
Publication statusPublished - Nov 1996

Fingerprint

Biocatalysis
Pseudomonas
Lipase
heptane
Pseudomonas fluorescens
Temperature
synthesis
Organic solvents
temperature
Esters
esters
enzyme activity
Water
Enzymes
water
biocatalysis
butyl caprylate
n-heptane

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Tan, Sze ; Owusu-Apenten, Richard ; Knapp, JS. / Low temperature organic phase biocatalysis using cold-adapted lipase from psychrotrophic Pseudomonas P38. 1996 ; Vol. 57, No. 3. pp. 415-418.
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title = "Low temperature organic phase biocatalysis using cold-adapted lipase from psychrotrophic Pseudomonas P38",
abstract = "Lipase produced by a psychrotroph, Pseudomonas fluorescens P38, was found to catalyse the synthesis of butyl caprylate in n-heptane at low temperatures. The optimum yield of ester synthesis was 75{\%} at 20 °C with an organic phase water concentration of 0.25{\%} (v/v). The results are discussed in terms of the structural flexibility of psychrotroph-derived lipase and the activity of this enzyme within a nearly anhydrous organic solvent phase.",
author = "Sze Tan and Richard Owusu-Apenten and JS Knapp",
note = "Reference text: Bjarnason and Asgeirsson, 1993. J.B. Bjarnason and B. Asgeirsson, Psychrophilic proteolytic enzymes from Atlantic cod: their characteristics and applications. Genet. Eng. Biotechnol. 13 1 (1993), pp. 31–39. Bucky et al., 1987. A.R. Bucky, D.S. Robinson and P.R. Hayes, Factors affecting the heat stability of lipase produced by a strain of Pseudomonas fluorescens. Food Chem. 23 (1987), pp. 159–173. Abstract | Article | PDF (668 K) | View Record in Scopus | Cited By in Scopus (3) Boreix et al., 1992. F. Boreix, F. Monot and J. Vandecasteele, Strategies for enzymatic esterification in organic solvents: comparison of microaqueous, biphasic, and micellar systems. Enzyme Microb. Technol. 14 (1992), pp. 791–797. Dias et al., 1991. S.F. Dias, L. Vilas-Boas, J.M.S. Cambral and M.M.R. Fonseca, Production of ethyl butyrate by Candida rugosa lipase immobilised in polyurethane. Biocatalysis 5 (1991), pp. 21–34. Full Text via CrossRef Dordick, 1989. D.S. Dordick, Enzymatic catalysis in monophasic organic solvents. Enzyme Microb. Technol. 11 (1989), pp. 194–210. Dring and Fox, 1983. R. Dring and P.F. Fox, Purification and characterisation of a heat stable lipase from Pseudomonas fluorescens AFT 29. Irish J. Food Sci. 7 (1983), pp. 157–171. Feller et al., 1990. G. Feller, M. Thirty, J.L. Arpigny, M. Mergeay and C. Gerday, Lipases from psychrotrophic Antarctic bacteria. FEMS Microbiol. Lett. 66 (1990), pp. 239–244. View Record in Scopus | Cited By in Scopus (37) Feller et al., 1992. G. Feller, T. Lonhienne, C. Deroanne, C. Libioulle, J. Van Beeumens and C. Gerday, Purification, characterisation, and nucleotide sequence of the thermolabile α-amylase from the Antarctic psychrotroph Altermonas haloplanctis A23. J. Biol. Chem. 267 8 (1992), pp. 5217–5221. View Record in Scopus | Cited By in Scopus (105) Feller et al., 1994. G. Feller, F. Payan, F. Theys, M. Qian, R. Haser and C. Gerday, Stability and structural analysis of α-amylase from the Antarctic psychrophile Altermonas haloplanctis A23. Eur. J. Biochem. 222 (1994), pp. 441–447. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (138) Gounot, 1991. A. Gounot, Bacterial life at low temperature: physiological aspects and biotechnological implications. J. Appl. Bacteriol. 71 (1991), pp. 386–397. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (103) Jaenicke, 1990. R. Jaenicke, Protein structure and function at low temperatures. Philos. Trans. R. Soc. Lond. B 326 (1990), pp. 535–553. Full Text via CrossRef Klibanov, 1986. A.M. Klibanov, Enzymes that work in organic solvents. Chemtech (1986), pp. 354–359 June . View Record in Scopus | Cited By in Scopus (326) Laane et al., 1986. C. Laane, S. Boeren, R. Hilhorst and C. Veeger, Optimisation of biocatalysis in organic media. In: C. Laane, J. Tramper and M.D. Lilly, Editors, Biocatalysis in Organic Media, Elsevier, Amsterdam (1986), pp. 65–84. Langrand et al., 1988. G. Langrand, C. Triantaphylides and J. Baratti, Lipase catalyzed formation of flavour esters. Biotechnol. Lett. 10 8 (1988), pp. 549–554. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (72) Owusu and Cowan, 1989. R.K. Owusu and D.A. Cowan, Correlation between microbial protein thermostability and resistance to denaturation in aqueous:organic solvent two phase systems. Enzyme Microb. Technol. 11 (1989), pp. 568–574. Abstract | PDF (679 K) | View Record in Scopus | Cited By in Scopus (42) Owusu and Cowan, 1990. R.K. Owusu and D.A. Cowan, Biocatalysis in organic solvent systmes using thermostable enzymes: esterase-catalysed transesterification of D-L-tyrosine p-nitrophenyl ester. Enzyme Microb. Technol. 12 (1990), pp. 374–377. Abstract | PDF (352 K) | View Record in Scopus | Cited By in Scopus (5) Owusu et al., 1991. R.K. Owusu, A. Makhzoum and J.S. Knapp, The thermodynamic stability of lipase and proteases from psychrotrophic bacteria. Food Chem. 39 (1991), pp. 187–195. Abstract | PDF (466 K) | View Record in Scopus | Cited By in Scopus (9) Owusu et al., 1992. R.K. Owusu, A. Makhzoum and J.S. Knapp, Heat inactivation of lipase from psychrotrophic Pseudomonas fluorescens P38 : activation parameters and enzyme stability at low or ultra-high temperatures. In: Food Chem. 44 (1992), pp. 261–268. Abstract | Article | PDF (719 K) | View Record in Scopus | Cited By in Scopus (34) Manjon et al., 1991. A. Manjon, J.L. Iborra and A. Acroas, Short-chain flavour esters synthesis by immobilised lipase in organic media. Biotechnol Lett. 13 5 (1991), pp. 339–344. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (64) McKellar, 1989. R.C. McKellar, Editor, Enzymes of Psychrotrophs in Raw Food, CRC Press, Boca Raton, FL (1989). Shaw and Lo, 1994. J.F. Shaw and S. Lo, Production of propylene glycol fatty acid monoesters by lipase-catalyzed reactions in organic solvents. J. Am. Oil Chem. Soc. 71 7 (1994), pp. 715–719. View Record in Scopus | Cited By in Scopus (16) Stead, 1986. D. Stead, Microbial lipases: their characteristics, role in food spoilage. J. Dairy Res. 53 (1986), pp. 481–505. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (74) Van Straten et al., 1977. S. Van Straten, F.L. De Vrijer and J.C. de Beauveser, Editors, Volatile Compounds in Food (4th edn. ed.),, C.I.N.F.R., Zeist, Netherlands (1977). Volkin et al., 1991. D.B. Volkin, A. Staubli, R. Lander and A.M. Klibanov, Enzyme thermoinactivation in anhydrous organic solvents. Biotechnol. Bioeng. 37 (1991), pp. 843–853. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (114) Welsh et al., 1990. F.W. Welsh, R.E. Williams and H. Dawson, Lipase mediated synthesis of low molecular weight flavour esters. J. Food Sci. 55 6 (1990), pp. 1679–1682. Full Text via CrossRef Winkler and Stuckman, 1979. U.K. Winkler and M. Stuckman, Polysaccharide enhancement of exolipase formation by S. marcescens. J. Bacteriol. 138 (1979), pp. 663–670. View Record in Scopus | Cited By in Scopus (280) Zaks and Klibanov, 1984. A. Zaks and A.M. Klibanov, Enzymatic catalysis in organic media at 100 °C. Science 224 (1984), pp. 1249–1251. View Record in Scopus | Cited By in Scopus (364) Zaks and Russell, 1988. A. Zaks and A.J. Russell, Enzymes in organic solvents: properties and applications. J. Biotechnol. 8 (1988), pp. 259–270. View Record in Scopus | Cited By in Scopus (62)",
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month = "11",
doi = "10.1016/0308-8146(95)00243-X",
language = "English",
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Low temperature organic phase biocatalysis using cold-adapted lipase from psychrotrophic Pseudomonas P38. / Tan, Sze; Owusu-Apenten, Richard; Knapp, JS.

Vol. 57, No. 3, 11.1996, p. 415-418.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Low temperature organic phase biocatalysis using cold-adapted lipase from psychrotrophic Pseudomonas P38

AU - Tan, Sze

AU - Owusu-Apenten, Richard

AU - Knapp, JS

N1 - Reference text: Bjarnason and Asgeirsson, 1993. J.B. Bjarnason and B. Asgeirsson, Psychrophilic proteolytic enzymes from Atlantic cod: their characteristics and applications. Genet. Eng. Biotechnol. 13 1 (1993), pp. 31–39. Bucky et al., 1987. A.R. Bucky, D.S. Robinson and P.R. Hayes, Factors affecting the heat stability of lipase produced by a strain of Pseudomonas fluorescens. Food Chem. 23 (1987), pp. 159–173. Abstract | Article | PDF (668 K) | View Record in Scopus | Cited By in Scopus (3) Boreix et al., 1992. F. Boreix, F. Monot and J. Vandecasteele, Strategies for enzymatic esterification in organic solvents: comparison of microaqueous, biphasic, and micellar systems. Enzyme Microb. Technol. 14 (1992), pp. 791–797. Dias et al., 1991. S.F. Dias, L. Vilas-Boas, J.M.S. Cambral and M.M.R. Fonseca, Production of ethyl butyrate by Candida rugosa lipase immobilised in polyurethane. Biocatalysis 5 (1991), pp. 21–34. Full Text via CrossRef Dordick, 1989. D.S. Dordick, Enzymatic catalysis in monophasic organic solvents. Enzyme Microb. Technol. 11 (1989), pp. 194–210. Dring and Fox, 1983. R. Dring and P.F. Fox, Purification and characterisation of a heat stable lipase from Pseudomonas fluorescens AFT 29. Irish J. Food Sci. 7 (1983), pp. 157–171. Feller et al., 1990. G. Feller, M. Thirty, J.L. Arpigny, M. Mergeay and C. Gerday, Lipases from psychrotrophic Antarctic bacteria. FEMS Microbiol. Lett. 66 (1990), pp. 239–244. View Record in Scopus | Cited By in Scopus (37) Feller et al., 1992. G. Feller, T. Lonhienne, C. Deroanne, C. Libioulle, J. Van Beeumens and C. Gerday, Purification, characterisation, and nucleotide sequence of the thermolabile α-amylase from the Antarctic psychrotroph Altermonas haloplanctis A23. J. Biol. Chem. 267 8 (1992), pp. 5217–5221. View Record in Scopus | Cited By in Scopus (105) Feller et al., 1994. G. Feller, F. Payan, F. Theys, M. Qian, R. Haser and C. Gerday, Stability and structural analysis of α-amylase from the Antarctic psychrophile Altermonas haloplanctis A23. Eur. J. Biochem. 222 (1994), pp. 441–447. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (138) Gounot, 1991. A. Gounot, Bacterial life at low temperature: physiological aspects and biotechnological implications. J. Appl. Bacteriol. 71 (1991), pp. 386–397. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (103) Jaenicke, 1990. R. Jaenicke, Protein structure and function at low temperatures. Philos. Trans. R. Soc. Lond. B 326 (1990), pp. 535–553. Full Text via CrossRef Klibanov, 1986. A.M. Klibanov, Enzymes that work in organic solvents. Chemtech (1986), pp. 354–359 June . View Record in Scopus | Cited By in Scopus (326) Laane et al., 1986. C. Laane, S. Boeren, R. Hilhorst and C. Veeger, Optimisation of biocatalysis in organic media. In: C. Laane, J. Tramper and M.D. Lilly, Editors, Biocatalysis in Organic Media, Elsevier, Amsterdam (1986), pp. 65–84. Langrand et al., 1988. G. Langrand, C. Triantaphylides and J. Baratti, Lipase catalyzed formation of flavour esters. Biotechnol. Lett. 10 8 (1988), pp. 549–554. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (72) Owusu and Cowan, 1989. R.K. Owusu and D.A. Cowan, Correlation between microbial protein thermostability and resistance to denaturation in aqueous:organic solvent two phase systems. Enzyme Microb. Technol. 11 (1989), pp. 568–574. Abstract | PDF (679 K) | View Record in Scopus | Cited By in Scopus (42) Owusu and Cowan, 1990. R.K. Owusu and D.A. Cowan, Biocatalysis in organic solvent systmes using thermostable enzymes: esterase-catalysed transesterification of D-L-tyrosine p-nitrophenyl ester. Enzyme Microb. Technol. 12 (1990), pp. 374–377. Abstract | PDF (352 K) | View Record in Scopus | Cited By in Scopus (5) Owusu et al., 1991. R.K. Owusu, A. Makhzoum and J.S. Knapp, The thermodynamic stability of lipase and proteases from psychrotrophic bacteria. Food Chem. 39 (1991), pp. 187–195. Abstract | PDF (466 K) | View Record in Scopus | Cited By in Scopus (9) Owusu et al., 1992. R.K. Owusu, A. Makhzoum and J.S. Knapp, Heat inactivation of lipase from psychrotrophic Pseudomonas fluorescens P38 : activation parameters and enzyme stability at low or ultra-high temperatures. In: Food Chem. 44 (1992), pp. 261–268. Abstract | Article | PDF (719 K) | View Record in Scopus | Cited By in Scopus (34) Manjon et al., 1991. A. Manjon, J.L. Iborra and A. Acroas, Short-chain flavour esters synthesis by immobilised lipase in organic media. Biotechnol Lett. 13 5 (1991), pp. 339–344. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (64) McKellar, 1989. R.C. McKellar, Editor, Enzymes of Psychrotrophs in Raw Food, CRC Press, Boca Raton, FL (1989). Shaw and Lo, 1994. J.F. Shaw and S. Lo, Production of propylene glycol fatty acid monoesters by lipase-catalyzed reactions in organic solvents. J. Am. Oil Chem. Soc. 71 7 (1994), pp. 715–719. View Record in Scopus | Cited By in Scopus (16) Stead, 1986. D. Stead, Microbial lipases: their characteristics, role in food spoilage. J. Dairy Res. 53 (1986), pp. 481–505. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (74) Van Straten et al., 1977. S. Van Straten, F.L. De Vrijer and J.C. de Beauveser, Editors, Volatile Compounds in Food (4th edn. ed.),, C.I.N.F.R., Zeist, Netherlands (1977). Volkin et al., 1991. D.B. Volkin, A. Staubli, R. Lander and A.M. Klibanov, Enzyme thermoinactivation in anhydrous organic solvents. Biotechnol. Bioeng. 37 (1991), pp. 843–853. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (114) Welsh et al., 1990. F.W. Welsh, R.E. Williams and H. Dawson, Lipase mediated synthesis of low molecular weight flavour esters. J. Food Sci. 55 6 (1990), pp. 1679–1682. Full Text via CrossRef Winkler and Stuckman, 1979. U.K. Winkler and M. Stuckman, Polysaccharide enhancement of exolipase formation by S. marcescens. J. Bacteriol. 138 (1979), pp. 663–670. View Record in Scopus | Cited By in Scopus (280) Zaks and Klibanov, 1984. A. Zaks and A.M. Klibanov, Enzymatic catalysis in organic media at 100 °C. Science 224 (1984), pp. 1249–1251. View Record in Scopus | Cited By in Scopus (364) Zaks and Russell, 1988. A. Zaks and A.J. Russell, Enzymes in organic solvents: properties and applications. J. Biotechnol. 8 (1988), pp. 259–270. View Record in Scopus | Cited By in Scopus (62)

PY - 1996/11

Y1 - 1996/11

N2 - Lipase produced by a psychrotroph, Pseudomonas fluorescens P38, was found to catalyse the synthesis of butyl caprylate in n-heptane at low temperatures. The optimum yield of ester synthesis was 75% at 20 °C with an organic phase water concentration of 0.25% (v/v). The results are discussed in terms of the structural flexibility of psychrotroph-derived lipase and the activity of this enzyme within a nearly anhydrous organic solvent phase.

AB - Lipase produced by a psychrotroph, Pseudomonas fluorescens P38, was found to catalyse the synthesis of butyl caprylate in n-heptane at low temperatures. The optimum yield of ester synthesis was 75% at 20 °C with an organic phase water concentration of 0.25% (v/v). The results are discussed in terms of the structural flexibility of psychrotroph-derived lipase and the activity of this enzyme within a nearly anhydrous organic solvent phase.

U2 - 10.1016/0308-8146(95)00243-X

DO - 10.1016/0308-8146(95)00243-X

M3 - Article

VL - 57

SP - 415

EP - 418

IS - 3

ER -