Long-term high copper intake: effects on indexes of copper status, antioxidant status, and immune function in young men

JR Turnlund, RA Jacob, CL Keen, JJ Strain, DS Kelley, JM Domek, WR Keyes, JL Ensunsa, J Lykkesfeldt, J Coulter

Research output: Contribution to journalArticle

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Abstract

Background: Short-term high copper intake does not appear to affect indexes of copper status or functions related to copper status, but the effects of long-term high copper intake are unknown. Objective: A study was conducted in men to determine the effect of long-term high copper intake on indexes of copper status, oxidant damage, and immune function. Design: Nine men were confined to a metabolic research unit (MRU) for 18 d and were fed a 3-d rotating menu providing an average of 1.6 ing Cu/d. The men continued the study under free-living conditions for 129 d and supplemented their usual diets with 7 mg Cu/d. The men then returned to the MRU for 18 d of the same diet as during the first period, except that copper intake was 7.8 mg/d. Plasma copper, ceruloplasmin activity, ceruloplasmin protein, plasma malondialdehyde, benzylamine oxidase activity, erythrocyte superoxide dismutase, hair copper, urinary copper, and urinary thiobarbituric acid-reactive substances were measured during each MRU period. Results: Ceruloplasmin activity, benzylamine oxidase, and superoxide dismutase were significantly higher at the end of the second MRU period than at the end of the first. Urinary copper excretion, hair copper concentrations, and urinary thiobarbituric acid-reactive substances were significantly higher during the second MRU period than during the first. Polymorphonuclear cell count, the percentage of white blood cells, lymphocyte count, and interleukin 2R were affected by copper supplementation. Antibody titer for the Beijing strain of influenza virus was significantly lower in supplemented subjects after immunization than in unsupplemented control subjects. Conclusions: Under highly controlled conditions, long-term high copper intake results in increases in some indexes of copper status, alters an index of oxidant stress, and affects several indexes of immune function. The physiologic implications of these changes are unknown.
LanguageEnglish
Pages1037-1044
JournalAmerican Journal of Clinical Nutrition
Volume79
Issue number6
Publication statusPublished - Jun 2004

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Copper
Antioxidants
Ceruloplasmin
Benzylamine Oxidase
Research
Thiobarbituric Acid Reactive Substances
Oxidants
Hair
Superoxide Dismutase
Diet
Interleukins
Social Conditions
Lymphocyte Count
Orthomyxoviridae
Malondialdehyde
Leukocyte Count
Blood Proteins
Immunization
Cell Count
Erythrocytes

Keywords

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Cite this

Turnlund, JR ; Jacob, RA ; Keen, CL ; Strain, JJ ; Kelley, DS ; Domek, JM ; Keyes, WR ; Ensunsa, JL ; Lykkesfeldt, J ; Coulter, J. / Long-term high copper intake: effects on indexes of copper status, antioxidant status, and immune function in young men. In: American Journal of Clinical Nutrition. 2004 ; Vol. 79, No. 6. pp. 1037-1044.
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abstract = "Background: Short-term high copper intake does not appear to affect indexes of copper status or functions related to copper status, but the effects of long-term high copper intake are unknown. Objective: A study was conducted in men to determine the effect of long-term high copper intake on indexes of copper status, oxidant damage, and immune function. Design: Nine men were confined to a metabolic research unit (MRU) for 18 d and were fed a 3-d rotating menu providing an average of 1.6 ing Cu/d. The men continued the study under free-living conditions for 129 d and supplemented their usual diets with 7 mg Cu/d. The men then returned to the MRU for 18 d of the same diet as during the first period, except that copper intake was 7.8 mg/d. Plasma copper, ceruloplasmin activity, ceruloplasmin protein, plasma malondialdehyde, benzylamine oxidase activity, erythrocyte superoxide dismutase, hair copper, urinary copper, and urinary thiobarbituric acid-reactive substances were measured during each MRU period. Results: Ceruloplasmin activity, benzylamine oxidase, and superoxide dismutase were significantly higher at the end of the second MRU period than at the end of the first. Urinary copper excretion, hair copper concentrations, and urinary thiobarbituric acid-reactive substances were significantly higher during the second MRU period than during the first. Polymorphonuclear cell count, the percentage of white blood cells, lymphocyte count, and interleukin 2R were affected by copper supplementation. Antibody titer for the Beijing strain of influenza virus was significantly lower in supplemented subjects after immunization than in unsupplemented control subjects. Conclusions: Under highly controlled conditions, long-term high copper intake results in increases in some indexes of copper status, alters an index of oxidant stress, and affects several indexes of immune function. The physiologic implications of these changes are unknown.",
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Turnlund, JR, Jacob, RA, Keen, CL, Strain, JJ, Kelley, DS, Domek, JM, Keyes, WR, Ensunsa, JL, Lykkesfeldt, J & Coulter, J 2004, 'Long-term high copper intake: effects on indexes of copper status, antioxidant status, and immune function in young men', American Journal of Clinical Nutrition, vol. 79, no. 6, pp. 1037-1044.

Long-term high copper intake: effects on indexes of copper status, antioxidant status, and immune function in young men. / Turnlund, JR; Jacob, RA; Keen, CL; Strain, JJ; Kelley, DS; Domek, JM; Keyes, WR; Ensunsa, JL; Lykkesfeldt, J; Coulter, J.

In: American Journal of Clinical Nutrition, Vol. 79, No. 6, 06.2004, p. 1037-1044.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Long-term high copper intake: effects on indexes of copper status, antioxidant status, and immune function in young men

AU - Turnlund, JR

AU - Jacob, RA

AU - Keen, CL

AU - Strain, JJ

AU - Kelley, DS

AU - Domek, JM

AU - Keyes, WR

AU - Ensunsa, JL

AU - Lykkesfeldt, J

AU - Coulter, J

PY - 2004/6

Y1 - 2004/6

N2 - Background: Short-term high copper intake does not appear to affect indexes of copper status or functions related to copper status, but the effects of long-term high copper intake are unknown. Objective: A study was conducted in men to determine the effect of long-term high copper intake on indexes of copper status, oxidant damage, and immune function. Design: Nine men were confined to a metabolic research unit (MRU) for 18 d and were fed a 3-d rotating menu providing an average of 1.6 ing Cu/d. The men continued the study under free-living conditions for 129 d and supplemented their usual diets with 7 mg Cu/d. The men then returned to the MRU for 18 d of the same diet as during the first period, except that copper intake was 7.8 mg/d. Plasma copper, ceruloplasmin activity, ceruloplasmin protein, plasma malondialdehyde, benzylamine oxidase activity, erythrocyte superoxide dismutase, hair copper, urinary copper, and urinary thiobarbituric acid-reactive substances were measured during each MRU period. Results: Ceruloplasmin activity, benzylamine oxidase, and superoxide dismutase were significantly higher at the end of the second MRU period than at the end of the first. Urinary copper excretion, hair copper concentrations, and urinary thiobarbituric acid-reactive substances were significantly higher during the second MRU period than during the first. Polymorphonuclear cell count, the percentage of white blood cells, lymphocyte count, and interleukin 2R were affected by copper supplementation. Antibody titer for the Beijing strain of influenza virus was significantly lower in supplemented subjects after immunization than in unsupplemented control subjects. Conclusions: Under highly controlled conditions, long-term high copper intake results in increases in some indexes of copper status, alters an index of oxidant stress, and affects several indexes of immune function. The physiologic implications of these changes are unknown.

AB - Background: Short-term high copper intake does not appear to affect indexes of copper status or functions related to copper status, but the effects of long-term high copper intake are unknown. Objective: A study was conducted in men to determine the effect of long-term high copper intake on indexes of copper status, oxidant damage, and immune function. Design: Nine men were confined to a metabolic research unit (MRU) for 18 d and were fed a 3-d rotating menu providing an average of 1.6 ing Cu/d. The men continued the study under free-living conditions for 129 d and supplemented their usual diets with 7 mg Cu/d. The men then returned to the MRU for 18 d of the same diet as during the first period, except that copper intake was 7.8 mg/d. Plasma copper, ceruloplasmin activity, ceruloplasmin protein, plasma malondialdehyde, benzylamine oxidase activity, erythrocyte superoxide dismutase, hair copper, urinary copper, and urinary thiobarbituric acid-reactive substances were measured during each MRU period. Results: Ceruloplasmin activity, benzylamine oxidase, and superoxide dismutase were significantly higher at the end of the second MRU period than at the end of the first. Urinary copper excretion, hair copper concentrations, and urinary thiobarbituric acid-reactive substances were significantly higher during the second MRU period than during the first. Polymorphonuclear cell count, the percentage of white blood cells, lymphocyte count, and interleukin 2R were affected by copper supplementation. Antibody titer for the Beijing strain of influenza virus was significantly lower in supplemented subjects after immunization than in unsupplemented control subjects. Conclusions: Under highly controlled conditions, long-term high copper intake results in increases in some indexes of copper status, alters an index of oxidant stress, and affects several indexes of immune function. The physiologic implications of these changes are unknown.

KW - n/a

M3 - Article

VL - 79

SP - 1037

EP - 1044

JO - American Journal of Clinical Nutrition

T2 - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

SN - 0002-9165

IS - 6

ER -