Long-term beneficial effects of vanadate, tungstate, and molybdate on insulin secretion and function of cultured beta cells

HK Liu, BD Green, Neville McClenaghan, Janie McCluskey, Peter Flatt

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ultratrace elements vanadate, tungstate, and molybdate exhibit significant antihyperglycemic effects in both type 1 and 2 diabetic animals, but possible effects on the function of pancreatic beta cells are understudied. In the present study, clonal BRIN BD11 cells were cultured for 3 days with each ultratrace element to establish doses lacking detrimental effects on viable beta cell mass. Vanadate treatment ( 4 mumol/L) had no effect on cellular insulin content but improved glucose-induced insulin secretory responsiveness. However, insulin secretion mediated by PKA and PKC activation was desensitized in vanadate-treated cells. Culture with tungstate ( 300 mumol/L) and molybdate ( 1 mmol/L) increased cellular insulin content and enhanced basal insulin release and the responsiveness to glucose and a wide range of other secretagogues. These observations suggest significant effects of ultratrace elements on pancreatic beta cells that may contribute to their antihyperglycemic action.
Original languageEnglish
Pages (from-to)364-368
JournalPancreas
Volume28
Issue number4
Publication statusPublished - May 2004

Fingerprint Dive into the research topics of 'Long-term beneficial effects of vanadate, tungstate, and molybdate on insulin secretion and function of cultured beta cells'. Together they form a unique fingerprint.

  • Cite this