Long-term beneficial effects of vanadate, tungstate, and molybdate on insulin secretion and function of cultured beta cells

HK Liu, BD Green, Neville McClenaghan, Janie McCluskey, Peter Flatt

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Abstract

The ultratrace elements vanadate, tungstate, and molybdate exhibit significant antihyperglycemic effects in both type 1 and 2 diabetic animals, but possible effects on the function of pancreatic beta cells are understudied. In the present study, clonal BRIN BD11 cells were cultured for 3 days with each ultratrace element to establish doses lacking detrimental effects on viable beta cell mass. Vanadate treatment ( 4 mumol/L) had no effect on cellular insulin content but improved glucose-induced insulin secretory responsiveness. However, insulin secretion mediated by PKA and PKC activation was desensitized in vanadate-treated cells. Culture with tungstate ( 300 mumol/L) and molybdate ( 1 mmol/L) increased cellular insulin content and enhanced basal insulin release and the responsiveness to glucose and a wide range of other secretagogues. These observations suggest significant effects of ultratrace elements on pancreatic beta cells that may contribute to their antihyperglycemic action.
LanguageEnglish
Pages364-368
JournalPancreas
Volume28
Issue number4
Publication statusPublished - May 2004

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Vanadates
Cultured Cells
Insulin
Insulin-Secreting Cells
Hypoglycemic Agents
Glucose
molybdate
tungstate

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Liu, HK ; Green, BD ; McClenaghan, Neville ; McCluskey, Janie ; Flatt, Peter. / Long-term beneficial effects of vanadate, tungstate, and molybdate on insulin secretion and function of cultured beta cells. In: Pancreas. 2004 ; Vol. 28, No. 4. pp. 364-368.
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Long-term beneficial effects of vanadate, tungstate, and molybdate on insulin secretion and function of cultured beta cells. / Liu, HK; Green, BD; McClenaghan, Neville; McCluskey, Janie; Flatt, Peter.

In: Pancreas, Vol. 28, No. 4, 05.2004, p. 364-368.

Research output: Contribution to journalArticle

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AU - Liu, HK

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