Lipopeptide Biosurfactants from Paenibacillus polymyxa Inhibit Single and Mixed Species Biofilms

Gerry A. Quinn, Aaron P. Maloy, Stephen McClean, Brian Carney, John W. Slater

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although biofilms are recognised as important in microbial colonisation, solutions to theirinhibition are predominantly based on planktonic assays. These solutions have limited efficacy against biofilms. Here, a series of biofilm-orientated tests were used to identify antibiofilm compounds from marine micro-flora. This led to the isolation of a complex of antibiofilm compounds from a Paenibacillus polymyxa extract (PPE). A combination of rpHPLC and mass spectrometry identified the principle components of PPE as fusaricidin B (LI-FO4b) and polymyxin D1 with minor contributions from surfactins. This complex (PPE) reduced the biofilm biomass of Bacillus subtilis, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus bovis. In contrast, ampicillin was only effective against S. aureus. PPE also inhibited a self-assembling marine biofilm (SAMB) in co-incubation assays by 99.3% ±1.9 and disrupted established SAMB by 72.4% ± 4.4, while ampicillin showed no significant reduction. The effectiveness of this complex of lipopeptides against single and multispecies biofilms suggests a future role in biofilm prevention strategies.
LanguageEnglish
Pages1151-1166
JournalBiofouling
Volume28
Issue number10
DOIs
Publication statusPublished - 2012

Fingerprint

Paenibacillus polymyxa
lipopeptides
Lipopeptides
biosurfactants
Biofilms
biofilm
extracts
Ampicillin
ampicillin
Staphylococcus aureus
surfactin
Streptococcus bovis
polymyxins
assay
Polymyxins
Micrococcus luteus
microbial colonization
assays
Bacillus subtilis
Pseudomonas aeruginosa

Cite this

Quinn, Gerry A. ; Maloy, Aaron P. ; McClean, Stephen ; Carney, Brian ; Slater, John W. / Lipopeptide Biosurfactants from Paenibacillus polymyxa Inhibit Single and Mixed Species Biofilms. In: Biofouling. 2012 ; Vol. 28, No. 10. pp. 1151-1166.
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abstract = "Although biofilms are recognised as important in microbial colonisation, solutions to theirinhibition are predominantly based on planktonic assays. These solutions have limited efficacy against biofilms. Here, a series of biofilm-orientated tests were used to identify antibiofilm compounds from marine micro-flora. This led to the isolation of a complex of antibiofilm compounds from a Paenibacillus polymyxa extract (PPE). A combination of rpHPLC and mass spectrometry identified the principle components of PPE as fusaricidin B (LI-FO4b) and polymyxin D1 with minor contributions from surfactins. This complex (PPE) reduced the biofilm biomass of Bacillus subtilis, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus bovis. In contrast, ampicillin was only effective against S. aureus. PPE also inhibited a self-assembling marine biofilm (SAMB) in co-incubation assays by 99.3{\%} ±1.9 and disrupted established SAMB by 72.4{\%} ± 4.4, while ampicillin showed no significant reduction. The effectiveness of this complex of lipopeptides against single and multispecies biofilms suggests a future role in biofilm prevention strategies.",
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Lipopeptide Biosurfactants from Paenibacillus polymyxa Inhibit Single and Mixed Species Biofilms. / Quinn, Gerry A.; Maloy, Aaron P.; McClean, Stephen; Carney, Brian; Slater, John W.

In: Biofouling, Vol. 28, No. 10, 2012, p. 1151-1166.

Research output: Contribution to journalArticle

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AU - Maloy, Aaron P.

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