Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects.

Mary Hannon-Fletcher; Maurice O'Kane; Ken Moles; Colin Weatherup; Christopher Barnett; Yvonne Barnett

Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects.

Mary Hannon-Fletcher, Maurice O'Kane, Ken Moles, Colin Weatherup, Christopher Barnett, Yvonne Barnett

Research output: Contribution to journal › Article › peer-review

Abstract

Increased production of reactive oxygen species in vivo can lead to cellular biomolecule damage. Such damage has been suggested to contribute to the pathogenesis of IDDM. In this study we used the alkaline comet assay to measure DNA damage (single-stranded DNA breaks and alkali-labile sites) in freshly isolated whole blood, lymphocytes, monocytes and neutrophils from 23 subjects with IDDM and 32 age- and sex-matched controls. Analysis of the results showed elevated levels of DNA damage (expressed as % comet tail DNA) in the lymphocyte (4.10±0.47; 3.22±0.22), monocyte (4.28±0.47; 3.49±0.18) and whole blood (4.93±0.51; 4.51±0.23) fractions from IDDM subjects compared to controls, respectively, but the increases observed were not statistically significant. However, we found significantly elevated basal levels of DNA damage in the neutrophil fraction (8.38±0.64; 4.07±0.23; p

Original language	English
Pages (from-to)	53-60
Journal	Mutation Research
Volume	460
Issue number	1
Publication status	Published (in print/issue) - Jun 2000

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{018896fc10a148dd8ef574079d46400b,

title = "Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects.",

abstract = "Increased production of reactive oxygen species in vivo can lead to cellular biomolecule damage. Such damage has been suggested to contribute to the pathogenesis of IDDM. In this study we used the alkaline comet assay to measure DNA damage (single-stranded DNA breaks and alkali-labile sites) in freshly isolated whole blood, lymphocytes, monocytes and neutrophils from 23 subjects with IDDM and 32 age- and sex-matched controls. Analysis of the results showed elevated levels of DNA damage (expressed as % comet tail DNA) in the lymphocyte (4.10±0.47; 3.22±0.22), monocyte (4.28±0.47; 3.49±0.18) and whole blood (4.93±0.51; 4.51±0.23) fractions from IDDM subjects compared to controls, respectively, but the increases observed were not statistically significant. However, we found significantly elevated basal levels of DNA damage in the neutrophil fraction (8.38±0.64; 4.07±0.23; p",

author = "Mary Hannon-Fletcher and Maurice O'Kane and Ken Moles and Colin Weatherup and Christopher Barnett and Yvonne Barnett",

year = "2000",

month = jun,

language = "English",

volume = "460",

pages = "53--60",

journal = "Mutation Research",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects.

AU - Hannon-Fletcher, Mary

AU - O'Kane, Maurice

AU - Moles, Ken

AU - Weatherup, Colin

AU - Barnett, Christopher

AU - Barnett, Yvonne

PY - 2000/6

Y1 - 2000/6

N2 - Increased production of reactive oxygen species in vivo can lead to cellular biomolecule damage. Such damage has been suggested to contribute to the pathogenesis of IDDM. In this study we used the alkaline comet assay to measure DNA damage (single-stranded DNA breaks and alkali-labile sites) in freshly isolated whole blood, lymphocytes, monocytes and neutrophils from 23 subjects with IDDM and 32 age- and sex-matched controls. Analysis of the results showed elevated levels of DNA damage (expressed as % comet tail DNA) in the lymphocyte (4.10±0.47; 3.22±0.22), monocyte (4.28±0.47; 3.49±0.18) and whole blood (4.93±0.51; 4.51±0.23) fractions from IDDM subjects compared to controls, respectively, but the increases observed were not statistically significant. However, we found significantly elevated basal levels of DNA damage in the neutrophil fraction (8.38±0.64; 4.07±0.23; p

AB - Increased production of reactive oxygen species in vivo can lead to cellular biomolecule damage. Such damage has been suggested to contribute to the pathogenesis of IDDM. In this study we used the alkaline comet assay to measure DNA damage (single-stranded DNA breaks and alkali-labile sites) in freshly isolated whole blood, lymphocytes, monocytes and neutrophils from 23 subjects with IDDM and 32 age- and sex-matched controls. Analysis of the results showed elevated levels of DNA damage (expressed as % comet tail DNA) in the lymphocyte (4.10±0.47; 3.22±0.22), monocyte (4.28±0.47; 3.49±0.18) and whole blood (4.93±0.51; 4.51±0.23) fractions from IDDM subjects compared to controls, respectively, but the increases observed were not statistically significant. However, we found significantly elevated basal levels of DNA damage in the neutrophil fraction (8.38±0.64; 4.07±0.23; p

M3 - Article

VL - 460

SP - 53

EP - 60

JO - Mutation Research

JF - Mutation Research

IS - 1

ER -

Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects.

Abstract

UN SDGs

Fingerprint

Cite this