Large–scale screening of molecules involved in virus–host interaction by specific compounds in Cherax quadricarinatus hematopoietic tissue cells

White spot syndrome virus is one of the major pathogens that seriously threaten crustacean aquaculture. However, knowledge about the molecular mechanism of virus–host interaction remains limited. In the present study, a library containing 303 compounds targeting specific molecules was used for large–scale screening for the first time. Cherax quadricarinatus hematopoietic tissue cells were used in the present study. Combining the analysis of viral infection and cell toxicity test, 30 compounds were demonstrated to be effective. Their targeted molecules were involved in the regulation of a variety of cell signaling pathways and participated in several cellular processes, such as Hedgehog and NF–κB cell signaling pathway and regulation of cellular ions. The present study provides valuable insights into the molecular mechanism of the viral pathogenesis and host immunity, and will help to advance disease control.