Abstract
| Language | English |
|---|---|
| Pages | 125-131 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 681 |
| Issue number | 1 |
| Publication status | Published - May 1996 |
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Large-volume sample stacking of selected drugs of forensic significance by capillary electrophoresis. / McGrath, G; Smyth, Franklin.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 681, No. 1, 05.1996, p. 125-131.Research output: Contribution to journal › Article
TY - JOUR
T1 - Large-volume sample stacking of selected drugs of forensic significance by capillary electrophoresis
AU - McGrath, G
AU - Smyth, Franklin
N1 - 13th Symposium on Biomedical Applications of Chromatography and Electrophoresis, PRAGUE, CZECH REPUBLIC, SEP 04-07, 1995
PY - 1996/5
Y1 - 1996/5
N2 - Large volume sample stacking capillary electrophoresis (LVSS-CE) and conventional capillary electrophoresis (CE) are compared for the separation of drugs of significance to forensic and clinical analyses. LVSS-CE for cations requires the use of an electroosmotic flow (EOF) modifier in conjunction with polarity switching to effect on-column concentration of an analyte and its subsequent migration in the capillary. The run buffer consists of 0.05 mol dm(-3) disodium tetraborate adjusted to pH 2.2 with orthophosphoric acid, and the EOF modifier is 0.002 mol dm(-3) cetyltrimethylammonium bromide. CE investigations used an identical run buffer minus the EOF modifier. LVSS-CE and CE investigations used injection times of 30 s and 3 s, respectively. Both modes of capillary electrophoresis are compared in terms of their limits of detection, efficiency, resolution and reproducibility. LVSS-CE is also applied to the analysis of a spiked urine sample.
AB - Large volume sample stacking capillary electrophoresis (LVSS-CE) and conventional capillary electrophoresis (CE) are compared for the separation of drugs of significance to forensic and clinical analyses. LVSS-CE for cations requires the use of an electroosmotic flow (EOF) modifier in conjunction with polarity switching to effect on-column concentration of an analyte and its subsequent migration in the capillary. The run buffer consists of 0.05 mol dm(-3) disodium tetraborate adjusted to pH 2.2 with orthophosphoric acid, and the EOF modifier is 0.002 mol dm(-3) cetyltrimethylammonium bromide. CE investigations used an identical run buffer minus the EOF modifier. LVSS-CE and CE investigations used injection times of 30 s and 3 s, respectively. Both modes of capillary electrophoresis are compared in terms of their limits of detection, efficiency, resolution and reproducibility. LVSS-CE is also applied to the analysis of a spiked urine sample.
M3 - Article
VL - 681
SP - 125
EP - 131
JO - Journal of Chromatography B: Biomedical Sciences and Applications
T2 - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 0378-4347
IS - 1
ER -