Large-volume sample stacking of selected drugs of forensic significance by capillary electrophoresis

G McGrath, Franklin Smyth

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    76 Citations (Scopus)

    Abstract

    Large volume sample stacking capillary electrophoresis (LVSS-CE) and conventional capillary electrophoresis (CE) are compared for the separation of drugs of significance to forensic and clinical analyses. LVSS-CE for cations requires the use of an electroosmotic flow (EOF) modifier in conjunction with polarity switching to effect on-column concentration of an analyte and its subsequent migration in the capillary. The run buffer consists of 0.05 mol dm(-3) disodium tetraborate adjusted to pH 2.2 with orthophosphoric acid, and the EOF modifier is 0.002 mol dm(-3) cetyltrimethylammonium bromide. CE investigations used an identical run buffer minus the EOF modifier. LVSS-CE and CE investigations used injection times of 30 s and 3 s, respectively. Both modes of capillary electrophoresis are compared in terms of their limits of detection, efficiency, resolution and reproducibility. LVSS-CE is also applied to the analysis of a spiked urine sample.
    LanguageEnglish
    Pages125-131
    JournalJournal of Chromatography B: Biomedical Sciences and Applications
    Volume681
    Issue number1
    Publication statusPublished - May 1996

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    Capillary electrophoresis
    Pharmaceutical Preparations
    Buffers
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    title = "Large-volume sample stacking of selected drugs of forensic significance by capillary electrophoresis",
    abstract = "Large volume sample stacking capillary electrophoresis (LVSS-CE) and conventional capillary electrophoresis (CE) are compared for the separation of drugs of significance to forensic and clinical analyses. LVSS-CE for cations requires the use of an electroosmotic flow (EOF) modifier in conjunction with polarity switching to effect on-column concentration of an analyte and its subsequent migration in the capillary. The run buffer consists of 0.05 mol dm(-3) disodium tetraborate adjusted to pH 2.2 with orthophosphoric acid, and the EOF modifier is 0.002 mol dm(-3) cetyltrimethylammonium bromide. CE investigations used an identical run buffer minus the EOF modifier. LVSS-CE and CE investigations used injection times of 30 s and 3 s, respectively. Both modes of capillary electrophoresis are compared in terms of their limits of detection, efficiency, resolution and reproducibility. LVSS-CE is also applied to the analysis of a spiked urine sample.",
    author = "G McGrath and Franklin Smyth",
    note = "13th Symposium on Biomedical Applications of Chromatography and Electrophoresis, PRAGUE, CZECH REPUBLIC, SEP 04-07, 1995",
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    journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
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    Large-volume sample stacking of selected drugs of forensic significance by capillary electrophoresis. / McGrath, G; Smyth, Franklin.

    In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 681, No. 1, 05.1996, p. 125-131.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Large-volume sample stacking of selected drugs of forensic significance by capillary electrophoresis

    AU - McGrath, G

    AU - Smyth, Franklin

    N1 - 13th Symposium on Biomedical Applications of Chromatography and Electrophoresis, PRAGUE, CZECH REPUBLIC, SEP 04-07, 1995

    PY - 1996/5

    Y1 - 1996/5

    N2 - Large volume sample stacking capillary electrophoresis (LVSS-CE) and conventional capillary electrophoresis (CE) are compared for the separation of drugs of significance to forensic and clinical analyses. LVSS-CE for cations requires the use of an electroosmotic flow (EOF) modifier in conjunction with polarity switching to effect on-column concentration of an analyte and its subsequent migration in the capillary. The run buffer consists of 0.05 mol dm(-3) disodium tetraborate adjusted to pH 2.2 with orthophosphoric acid, and the EOF modifier is 0.002 mol dm(-3) cetyltrimethylammonium bromide. CE investigations used an identical run buffer minus the EOF modifier. LVSS-CE and CE investigations used injection times of 30 s and 3 s, respectively. Both modes of capillary electrophoresis are compared in terms of their limits of detection, efficiency, resolution and reproducibility. LVSS-CE is also applied to the analysis of a spiked urine sample.

    AB - Large volume sample stacking capillary electrophoresis (LVSS-CE) and conventional capillary electrophoresis (CE) are compared for the separation of drugs of significance to forensic and clinical analyses. LVSS-CE for cations requires the use of an electroosmotic flow (EOF) modifier in conjunction with polarity switching to effect on-column concentration of an analyte and its subsequent migration in the capillary. The run buffer consists of 0.05 mol dm(-3) disodium tetraborate adjusted to pH 2.2 with orthophosphoric acid, and the EOF modifier is 0.002 mol dm(-3) cetyltrimethylammonium bromide. CE investigations used an identical run buffer minus the EOF modifier. LVSS-CE and CE investigations used injection times of 30 s and 3 s, respectively. Both modes of capillary electrophoresis are compared in terms of their limits of detection, efficiency, resolution and reproducibility. LVSS-CE is also applied to the analysis of a spiked urine sample.

    M3 - Article

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    SP - 125

    EP - 131

    JO - Journal of Chromatography B: Biomedical Sciences and Applications

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    SN - 0378-4347

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    ER -