Isolation, structural characterization and pharmacological activity of dog neuromedin U

Finbarr O'Harte; Charles S. Bockman; Peter W. Abel; J. Michael Conlon

doi:10.1016/0196-9781(91)90159-M

Isolation, structural characterization and pharmacological activity of dog neuromedin U

Finbarr O'Harte, Charles S. Bockman, Peter W. Abel, J. Michael Conlon

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64 Citations (Scopus)

Abstract

The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU-25) was established as: Phe-Arg-Leu-Asp-Glu-Glu-Phe-Gln-Gly-Pro¹⁰-Ile-Ala-Ser-Gln-Val-Arg-Arg-Gln-Phe-Leu²⁰-Phe-Arg-Pro-Arg-Asn-NH₂. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH₂. The sequence contains the substitution pGlu for Tyr¹ compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (EC₅₀ 10±2 nM; mean ± S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC₅₀ 16±5 nM) but the maximum response produced by the dog peptide was greater (58%; p<0.05) than that produced by pig NMU-8.

Original language	English
Pages (from-to)	11-15
Number of pages	5
Journal	Peptides
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/0196-9781(91)90159-M
Publication status	Published (in print/issue) - 1991

Funding

The work was supported in part by the Nebraska Heart Association. The authors thank Dr. Lars Thim, Novo Nordisk A/S, Bagsvaerd, Denmark for mass spectrometry measurements.

Keywords

Dog
Intestine
Neuromedin U
Uterus (rat)

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10.1016/0196-9781(91)90159-M

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title = "Isolation, structural characterization and pharmacological activity of dog neuromedin U",

abstract = "The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU-25) was established as: Phe-Arg-Leu-Asp-Glu-Glu-Phe-Gln-Gly-Pro10-Ile-Ala-Ser-Gln-Val-Arg-Arg-Gln-Phe-Leu20-Phe-Arg-Pro-Arg-Asn-NH2. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The sequence contains the substitution pGlu for Tyr1 compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (EC50 10±2 nM; mean ± S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC50 16±5 nM) but the maximum response produced by the dog peptide was greater (58\%; p<0.05) than that produced by pig NMU-8.",

keywords = "Dog, Intestine, Neuromedin U, Uterus (rat)",

author = "Finbarr O'Harte and Bockman, \{Charles S.\} and Abel, \{Peter W.\} and Conlon, \{J. Michael\}",

year = "1991",

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AU - Conlon, J. Michael

PY - 1991

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N2 - The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU-25) was established as: Phe-Arg-Leu-Asp-Glu-Glu-Phe-Gln-Gly-Pro10-Ile-Ala-Ser-Gln-Val-Arg-Arg-Gln-Phe-Leu20-Phe-Arg-Pro-Arg-Asn-NH2. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The sequence contains the substitution pGlu for Tyr1 compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (EC50 10±2 nM; mean ± S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC50 16±5 nM) but the maximum response produced by the dog peptide was greater (58%; p<0.05) than that produced by pig NMU-8.

AB - The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU-25) was established as: Phe-Arg-Leu-Asp-Glu-Glu-Phe-Gln-Gly-Pro10-Ile-Ala-Ser-Gln-Val-Arg-Arg-Gln-Phe-Leu20-Phe-Arg-Pro-Arg-Asn-NH2. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The sequence contains the substitution pGlu for Tyr1 compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (EC50 10±2 nM; mean ± S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC50 16±5 nM) but the maximum response produced by the dog peptide was greater (58%; p<0.05) than that produced by pig NMU-8.

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KW - Uterus (rat)

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Isolation, structural characterization and pharmacological activity of dog neuromedin U

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