Isolation, structural characterization and pharmacological activity of dog neuromedin U

Finbarr O'Harte, Charles S. Bockman, Peter W. Abel, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU-25) was established as: Phe-Arg-Leu-Asp-Glu-Glu-Phe-Gln-Gly-Pro10-Ile-Ala-Ser-Gln-Val-Arg-Arg-Gln-Phe-Leu20-Phe-Arg-Pro-Arg-Asn-NH2. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The sequence contains the substitution pGlu for Tyr1 compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (EC50 10±2 nM; mean ± S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC50 16±5 nM) but the maximum response produced by the dog peptide was greater (58%; p<0.05) than that produced by pig NMU-8.

Original languageEnglish
Pages (from-to)11-15
Number of pages5
JournalPeptides
Volume12
Issue number1
DOIs
Publication statusPublished - 1991

Keywords

  • Dog
  • Intestine
  • Neuromedin U
  • Uterus (rat)

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