Abstract
The neuromedin U-like immunoreactivity in an extract of dog small intestine was resolved by reversed-phase HPLC into two molecular forms. The primary structure of the larger form (NMU-25) was established as: Phe-Arg-Leu-Asp-Glu-Glu-Phe-Gln-Gly-Pro10-Ile-Ala-Ser-Gln-Val-Arg-Arg-Gln-Phe-Leu20-Phe-Arg-Pro-Arg-Asn-NH2. This sequence shows five substitutions relative to pig neuromedin U-25. The primary structure of the second peptide (NMU-8) was established as: pGlu-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The sequence contains the substitution pGlu for Tyr1 compared with pig neuromedin U-8. The potency of synthetic dog NMU-8 in contracting smooth muscle from the rat uterus (EC50 10±2 nM; mean ± S.E., n=6) was not significantly different from the corresponding potency of pig NMU-8 (EC50 16±5 nM) but the maximum response produced by the dog peptide was greater (58%; p<0.05) than that produced by pig NMU-8.
Original language | English |
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Pages (from-to) | 11-15 |
Number of pages | 5 |
Journal | Peptides |
Volume | 12 |
Issue number | 1 |
DOIs | |
Publication status | Published (in print/issue) - 1991 |
Keywords
- Dog
- Intestine
- Neuromedin U
- Uterus (rat)