Isolation of the opioid peptide Leu-Val-Val-hemorphin-7 from bronchoalveolar lavage fluid of a patient with non-small cell lung cancer

David Duethman, Naresh Dewan, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The decapeptide Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe was isolated in high yield (1.5 nmol/ml) from bronchoalveolar lavage (BAL) fluid from a patient with an adenocarcinoma of the lung. This peptide, termed LVV- hemorphin-7 represents residues 32-41 of the β-chain of hemoglobin and has been shown to be an endogenous ligand for opioid receptors. The N-terminal flanking peptide of LVV-hemorphin-7 [residues (1-31) of hemoglobin β-chain] was also isolated in high yield. Neither peptide was detected in BAL fluid from the tumor-free lung of the same patient or from patients with non- neoplastic inflammatory lung disease. LVV-hemorphin-7 was not identified in BAL fluid from seven additional patients with non-small cell lung cancer, indicating that the formation of the peptide is unlikely to be of any diagnostic significance. However, the ability of LVV-hemorphin-7 to inhibit angiotensin-converting enzyme suggests that its formation may be of pathophysiological significance in the regulation of tumor blood flow in certain patients. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalPeptides
Volume21
Issue number1
DOIs
Publication statusPublished (in print/issue) - Jan 2000

Keywords

  • Hemoglobin
  • Hemorphin
  • Non-small cell lung cancer

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