Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation

AS Aburuwaida, Ibrahim Banat, S Haditirto, A Salem, M Kadri

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

A gram-positive, nonfermentative, rod-shaped bacterium designated St-5, identified as Rhodococcus, was isolated from Kuwait soil. Grown on hydrocarbon, such as kerosene and n-paraffin, the bacterium produced surface-active compounds (biosurfactants). Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite. The ST-5 culture surface-active component is mainly glycolipid in nature. Whole-culture broth dropped surface tension to values below 27 mN/m and was stable during, exposure to high salinity (10% NaCl), elevated temperatures (120-degrees-C for 15 min) and a wide range of pH values. The culture broth was effective in recovering up to 86% of the residual oil from oil-saturated sand packs, indicating potential value in enhanced oil-recovery processes.
LanguageEnglish
Pages315-324
JournalACTA Biotechnologica
Volume11
Issue number4
Publication statusPublished - 1991

Fingerprint

Bacteria
Oils
Surface Tension
Surface tension
Gram-Positive Rods
Kerosene
Kuwait
Rhodococcus
Glycolipids
Salinity
Micelles
Metabolites
Hydrocarbons
Paraffin
Paraffins
Dilution
Sand
Soil
Soils
Recovery

Cite this

Aburuwaida, AS., Banat, I., Haditirto, S., Salem, A., & Kadri, M. (1991). Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation. ACTA Biotechnologica, 11(4), 315-324.
Aburuwaida, AS ; Banat, Ibrahim ; Haditirto, S ; Salem, A ; Kadri, M. / Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation. In: ACTA Biotechnologica. 1991 ; Vol. 11, No. 4. pp. 315-324.
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abstract = "A gram-positive, nonfermentative, rod-shaped bacterium designated St-5, identified as Rhodococcus, was isolated from Kuwait soil. Grown on hydrocarbon, such as kerosene and n-paraffin, the bacterium produced surface-active compounds (biosurfactants). Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite. The ST-5 culture surface-active component is mainly glycolipid in nature. Whole-culture broth dropped surface tension to values below 27 mN/m and was stable during, exposure to high salinity (10{\%} NaCl), elevated temperatures (120-degrees-C for 15 min) and a wide range of pH values. The culture broth was effective in recovering up to 86{\%} of the residual oil from oil-saturated sand packs, indicating potential value in enhanced oil-recovery processes.",
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Aburuwaida, AS, Banat, I, Haditirto, S, Salem, A & Kadri, M 1991, 'Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation', ACTA Biotechnologica, vol. 11, no. 4, pp. 315-324.

Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation. / Aburuwaida, AS; Banat, Ibrahim; Haditirto, S; Salem, A; Kadri, M.

In: ACTA Biotechnologica, Vol. 11, No. 4, 1991, p. 315-324.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation of Biosurfactant-Producing Bacteria Product Characterization, and Evaluation

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AU - Banat, Ibrahim

AU - Haditirto, S

AU - Salem, A

AU - Kadri, M

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AB - A gram-positive, nonfermentative, rod-shaped bacterium designated St-5, identified as Rhodococcus, was isolated from Kuwait soil. Grown on hydrocarbon, such as kerosene and n-paraffin, the bacterium produced surface-active compounds (biosurfactants). Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite. The ST-5 culture surface-active component is mainly glycolipid in nature. Whole-culture broth dropped surface tension to values below 27 mN/m and was stable during, exposure to high salinity (10% NaCl), elevated temperatures (120-degrees-C for 15 min) and a wide range of pH values. The culture broth was effective in recovering up to 86% of the residual oil from oil-saturated sand packs, indicating potential value in enhanced oil-recovery processes.

M3 - Article

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SP - 315

EP - 324

JO - ACTA Biotechnologica

T2 - ACTA Biotechnologica

JF - ACTA Biotechnologica

SN - 0138-4988

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ER -