This work evaluates the antimicrobial activity of the fractionated aqueous extract obtained from the aerial parts of aniseed. Fifteen fractions were collected by column chromatography using ethanol and water as mobile phases. All fractions were tested as potential antimicrobial agents against three common food-spoilage microorganisms: a yeast (Saccharomyces cerevisiae), a mold (Aspergillus awamori) and a bacterium (Lactobacillus delbrueckii subsp. bulgaricus). Fraction 2 exhibited the highest antimicrobial activity in both types of susceptibility test, and therefore, it was further studied for the identification of bioactive compounds. GC-MS analysis of Fraction 2 revealed the presence of threo-Anethole glycol. Based on laboratory-scale experiments, a process flowsheet for potential industrial-scale application is proposed combined with a techno-economic feasibility report. Fraction 2 with the detected compound threo-Anethole glycol showed promising results encouraging the attempts for industrial-scale application of antimicrobial compounds produced from aniseed.
|Number of pages||26|
|Journal||International Journal of Food Science and Technology|
|Early online date||9 May 2022|
|Publication status||E-pub ahead of print - 9 May 2022|
Bibliographical noteFunding Information:
The authors acknowledge the support of this work by the project ‘Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation – Food Innovation RI’ (MIS 5027222), which is implemented under the Action ‘Reinforcement of the Research and Innovation Infrastructure’, funded by the Operational Programme ‘Competitiveness, Entrepreneurship and Innovation’ (NSRF 2014‐2020) and co‐financed by Greece and the European Union (European Regional Development Fund).
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