Isolation and structural characterization of two novel peptides derived from proopiomelanocortin in the pituitary of the rainbow trout

Hélène Tollemer; Jérôme Leprince; Ludovic Galas; Frans Vandesande; Jean Claude Le Mevel; Marie Christine Tonon; J. Michael Conlon; Hubert Vaudry

doi:10.1006/bbrc.1997.7356

Isolation and structural characterization of two novel peptides derived from proopiomelanocortin in the pituitary of the rainbow trout

Hélène Tollemer
, Jérôme Leprince
, Ludovic Galas
, Frans Vandesande
, Jean Claude Le Mevel
, Marie Christine Tonon
, J. Michael Conlon
, Hubert Vaudry

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH₂. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.

Original language	English
Pages (from-to)	653-657
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	238
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1997.7356
Publication status	Published (in print/issue) - 18 Sept 1997

Funding

This work was supported by grants from INSERM (U 413), EU Human Capital and Mobility (Grant ERBCHRXCT 92-0017), NSF (IBN-84 188190) and the Conseil ReÂgional de Haute-Normandie. The authors wish to thank Dr Per F. Nielsen, Novo-Nordisk (Bagsvaerd, Denmark) for mass spectometry measurement. H.T. was a recipient of a fellowship from the EU Human Capital and Mobility Program. J.L. was a recipient of a fellowship from ORIL laboratories and the Conseil ReÂgional de Haute-Normandie. L.G. was a recipient of a fellowship from the MinisteÁre de l'Enseignement SupeÂrieur et de la Recherche.

Funders	Funder number
ERBCHRXCT 92-0017
National Science Foundation	IBN-84 188190
European Commission
Institut National de la Santé et de la Recherche Médicale, Paris, France	U 413

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10.1006/bbrc.1997.7356

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title = "Isolation and structural characterization of two novel peptides derived from proopiomelanocortin in the pituitary of the rainbow trout",

abstract = "The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.",

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AU - Tollemer, Hélène

AU - Leprince, Jérôme

AU - Galas, Ludovic

AU - Vandesande, Frans

AU - Mevel, Jean Claude Le

AU - Tonon, Marie Christine

AU - Conlon, J. Michael

AU - Vaudry, Hubert

PY - 1997/9/18

Y1 - 1997/9/18

N2 - The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.

AB - The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.

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VL - 238

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Isolation and structural characterization of two novel peptides derived from proopiomelanocortin in the pituitary of the rainbow trout

Abstract

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