Isolation and structural characterization of insulin from the holocephalan fish, Chimaera monstrosa (rabbit fish)

J. Michael Conlon, P. C. Andrews, Sture Falkmer, Lars Thima

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Insulin has been isolated from the pancreas of the holocephalan fish, Chimaera monstrosa (rabbit fish), and characterized by automated Edman degradation and fast atom bombardment mass spectrometry. The primary structure of rabbit fish insulin was identical to that of insulin from the holocephalan fish, Hydrolagus colliei (Pacific ratfish), and contained 21 residues in the A-chain and 38 residues in the B-chain. The amino acid compositions of both rabbit fish and ratfish insulins demonstrated a value consistently lower than that expected for the leucine content of the peptides. It is suggested, therefore, that the insulins were probably isolated as a mixture of the intact peptides and components lacking the C-terminal leucine residue in the B-chain.

Original languageEnglish
Pages (from-to)154-160
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume72
Issue number1
DOIs
Publication statusPublished (in print/issue) - Oct 1988

Fingerprint

Dive into the research topics of 'Isolation and structural characterization of insulin from the holocephalan fish, Chimaera monstrosa (rabbit fish)'. Together they form a unique fingerprint.

Cite this