TY - JOUR
T1 - Isolation and structural characterization of insulin from the holocephalan fish, Chimaera monstrosa (rabbit fish)
AU - Conlon, J. Michael
AU - Andrews, P. C.
AU - Falkmer, Sture
AU - Thima, Lars
PY - 1988/10
Y1 - 1988/10
N2 - Insulin has been isolated from the pancreas of the holocephalan fish, Chimaera monstrosa (rabbit fish), and characterized by automated Edman degradation and fast atom bombardment mass spectrometry. The primary structure of rabbit fish insulin was identical to that of insulin from the holocephalan fish, Hydrolagus colliei (Pacific ratfish), and contained 21 residues in the A-chain and 38 residues in the B-chain. The amino acid compositions of both rabbit fish and ratfish insulins demonstrated a value consistently lower than that expected for the leucine content of the peptides. It is suggested, therefore, that the insulins were probably isolated as a mixture of the intact peptides and components lacking the C-terminal leucine residue in the B-chain.
AB - Insulin has been isolated from the pancreas of the holocephalan fish, Chimaera monstrosa (rabbit fish), and characterized by automated Edman degradation and fast atom bombardment mass spectrometry. The primary structure of rabbit fish insulin was identical to that of insulin from the holocephalan fish, Hydrolagus colliei (Pacific ratfish), and contained 21 residues in the A-chain and 38 residues in the B-chain. The amino acid compositions of both rabbit fish and ratfish insulins demonstrated a value consistently lower than that expected for the leucine content of the peptides. It is suggested, therefore, that the insulins were probably isolated as a mixture of the intact peptides and components lacking the C-terminal leucine residue in the B-chain.
UR - http://www.scopus.com/inward/record.url?scp=0024095457&partnerID=8YFLogxK
U2 - 10.1016/0016-6480(88)90191-8
DO - 10.1016/0016-6480(88)90191-8
M3 - Article
C2 - 3053327
AN - SCOPUS:0024095457
SN - 0016-6480
VL - 72
SP - 154
EP - 160
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -