TY - JOUR
T1 - Isolation and structural characterization of calcitonin gene-related peptide from the brain and intestine of the frog, Rana ridibunda
AU - Michael Conlon, J.
AU - Tonon, Marie Christine
AU - Vaudry, Hubert
PY - 1993
Y1 - 1993
N2 - Calcitonin gene-related peptide (CGRP) was isolated in a single molecular form from an extract of the whole brain and from the small intestine of the European green frog, Rana ridibunda. The primary structure of the peptide was established as: Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr- His10-Arg-Leu-Ala-Asp-Phe-Leu-Ser-Arg-Ser-Gly20-Gly-Met-Ala-Lys-Asn-Asn-Phe-Val-Pro-Thr30-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2. Frog CGRP shows only two amino acid substitutions (Val22 → Met and Gly23 å Ala) compared with chicken CGRP, as deduced from the nucleotide sequence of a cloned cDNA. The Asp14 residue, which leads to enhanced biological potency in chicken CGRP, is also present in the frog peptide. The data show that the structure of CGRP has been strongly conserved during evolution of the vertebrates.
AB - Calcitonin gene-related peptide (CGRP) was isolated in a single molecular form from an extract of the whole brain and from the small intestine of the European green frog, Rana ridibunda. The primary structure of the peptide was established as: Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr- His10-Arg-Leu-Ala-Asp-Phe-Leu-Ser-Arg-Ser-Gly20-Gly-Met-Ala-Lys-Asn-Asn-Phe-Val-Pro-Thr30-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2. Frog CGRP shows only two amino acid substitutions (Val22 → Met and Gly23 å Ala) compared with chicken CGRP, as deduced from the nucleotide sequence of a cloned cDNA. The Asp14 residue, which leads to enhanced biological potency in chicken CGRP, is also present in the frog peptide. The data show that the structure of CGRP has been strongly conserved during evolution of the vertebrates.
KW - Brain
KW - Calcitonin gene-related peptide
KW - Frog
KW - Intestine
UR - http://www.scopus.com/inward/record.url?scp=0027298375&partnerID=8YFLogxK
U2 - 10.1016/0196-9781(93)90148-A
DO - 10.1016/0196-9781(93)90148-A
M3 - Article
C2 - 8332553
AN - SCOPUS:0027298375
SN - 0196-9781
VL - 14
SP - 581
EP - 586
JO - Peptides
JF - Peptides
IS - 3
ER -