Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

Research output: Contribution to journalArticle

1 Citation (Scopus)
32 Downloads (Pure)

Abstract

Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.
Original languageEnglish
Pages (from-to)211-218
JournalInternational Journal of Peptide Research and Therapeutics
Volume22
Early online date30 Nov 2015
DOIs
Publication statusPublished - Jun 2016

    Fingerprint

Keywords

  • Pseudechis australis
  • Pseudechis butleri
  • Elapid
  • Venom
  • Insulin secretion
  • Diabetes

Cite this