Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

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Abstract

Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.
LanguageEnglish
Pages211-218
JournalInternational Journal of Peptide Research and Therapeutics
Volume22
Early online date30 Nov 2015
DOIs
Publication statusPublished - Jun 2016

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Pseudechis australis
Pseudechis
venoms
insulin
phospholipase A2
neurotoxins
snakes
glucose
degradation
cells

Keywords

  • Pseudechis australis
  • Pseudechis butleri
  • Elapid
  • Venom
  • Insulin secretion
  • Diabetes

Cite this

@article{dd18b7b8482946098d5a3043c7eac7c8,
title = "Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom",
abstract = "Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.",
keywords = "Pseudechis australis, Pseudechis butleri, Elapid, Venom, Insulin secretion, Diabetes",
author = "Moore, {Sara W.M.} and Bhat, {Vikas K.} and Peter Flatt and Victor Gault and Stephen McClean",
year = "2016",
month = "6",
doi = "10.1007/s10989-015-9499-z",
language = "English",
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pages = "211--218",
journal = "International Journal of Peptide Research and Therapeutics",
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T1 - Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

AU - Moore, Sara W.M.

AU - Bhat, Vikas K.

AU - Flatt, Peter

AU - Gault, Victor

AU - McClean, Stephen

PY - 2016/6

Y1 - 2016/6

N2 - Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.

AB - Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.

KW - Pseudechis australis

KW - Pseudechis butleri

KW - Elapid

KW - Venom

KW - Insulin secretion

KW - Diabetes

U2 - 10.1007/s10989-015-9499-z

DO - 10.1007/s10989-015-9499-z

M3 - Article

VL - 22

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EP - 218

JO - International Journal of Peptide Research and Therapeutics

T2 - International Journal of Peptide Research and Therapeutics

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SN - 1573-3149

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