Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

Sara W.M. Moore; Vikas K. Bhat; Peter Flatt; Victor Gault; Stephen McClean

doi:10.1007/s10989-015-9499-z

Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

Sara W.M. Moore, Vikas K. Bhat, Peter Flatt, Victor Gault, Stephen McClean

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Abstract

Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.

Original language	English
Pages (from-to)	211-218
Journal	International Journal of Peptide Research and Therapeutics
Volume	22
Early online date	30 Nov 2015
DOIs	https://doi.org/10.1007/s10989-015-9499-z
Publication status	Published (in print/issue) - Jun 2016

Keywords

Pseudechis australis
Pseudechis butleri
Elapid
Venom
Insulin secretion
Diabetes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom",

abstract = "Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.",

keywords = "Pseudechis australis, Pseudechis butleri, Elapid, Venom, Insulin secretion, Diabetes",

author = "Moore, {Sara W.M.} and Bhat, {Vikas K.} and Peter Flatt and Victor Gault and Stephen McClean",

year = "2016",

month = jun,

doi = "10.1007/s10989-015-9499-z",

language = "English",

volume = "22",

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journal = "International Journal of Peptide Research and Therapeutics",

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T1 - Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

AU - Moore, Sara W.M.

AU - Bhat, Vikas K.

AU - Flatt, Peter

AU - Gault, Victor

AU - McClean, Stephen

PY - 2016/6

Y1 - 2016/6

N2 - Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.

AB - Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P <0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.

KW - Pseudechis australis

KW - Pseudechis butleri

KW - Elapid

KW - Venom

KW - Insulin secretion

KW - Diabetes

U2 - 10.1007/s10989-015-9499-z

DO - 10.1007/s10989-015-9499-z

M3 - Article

SN - 1573-3904

VL - 22

SP - 211

EP - 218

JO - International Journal of Peptide Research and Therapeutics

JF - International Journal of Peptide Research and Therapeutics

ER -

Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

Abstract

Keywords

UN SDGs

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