Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom.

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Abstract

Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.
Original languageEnglish
Pages (from-to)48 - 54
JournalToxicon
Volume101
Issue number0
DOIs
Publication statusPublished - 2015

Keywords

  • Diabetes

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    Stephen Mc Clean

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