TY - JOUR
T1 - Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom.
AU - Moore, Sara W.M.
AU - Bhat, Vikas K.
AU - Flatt, Peter
AU - Gault, Victor
AU - McClean, Stephen
PY - 2015
Y1 - 2015
N2 - Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.
AB - Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.
KW - Diabetes
UR - https://pure.ulster.ac.uk/en/publications/isolation-and-characterisation-of-insulin-releasing-compounds-fro-5
U2 - 10.1016/j.toxicon.2015.05.002
DO - 10.1016/j.toxicon.2015.05.002
M3 - Article
VL - 101
SP - 48
EP - 54
JO - Toxicon
JF - Toxicon
IS - 0
ER -
