Abstract
Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.
Original language | English |
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Pages (from-to) | 48 - 54 |
Journal | Toxicon |
Volume | 101 |
Issue number | 0 |
DOIs | |
Publication status | Published (in print/issue) - 2015 |
Keywords
- Diabetes
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Stephen McClean
- School of Biomedical Sciences - Head Of School Of Biomedical Sciences
- Faculty Of Life & Health Sciences - Full Professor
Person: Academic