Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom.

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Abstract

Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.
LanguageEnglish
Pages48 - 54
JournalToxicon
Volume101
Issue number0
DOIs
Publication statusPublished - 2015

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Crotalus
Snake Venoms
Venoms
Viperidae
Viper Venoms
Insulin
Disintegrins
Snakes
Phospholipases A2
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Serine Proteases
Medical problems
Chromatography
Gel Chromatography
Gels
Degradation
Peptides
Therapeutics
proteinase A2
In Vitro Techniques

Keywords

  • Diabetes

Cite this

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title = "Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom.",
abstract = "Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.",
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author = "Moore, {Sara W.M.} and Bhat, {Vikas K.} and Peter Flatt and Victor Gault and Stephen McClean",
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T1 - Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom.

AU - Moore, Sara W.M.

AU - Bhat, Vikas K.

AU - Flatt, Peter

AU - Gault, Victor

AU - McClean, Stephen

PY - 2015

Y1 - 2015

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AB - Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.

KW - Diabetes

U2 - 10.1016/j.toxicon.2015.05.002

DO - 10.1016/j.toxicon.2015.05.002

M3 - Article

VL - 101

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