Investigation of the electrochemical oxidation of clenbuterol at a porous carbon electrode, and its application to the determination of this beta-agonist in bovine hair by liquid chromatography with coulometric detection

GJ McGrath, E O'Kane, Franklin Smyth, F Tagliaro

    Research output: Contribution to journalArticle

    Abstract

    At pH 5, clenbuterol is oxidized at potentials > 650 mV to form a radical cation, two of which combine in a head to head manner to form a dimer. A method for the determination of this notably abused beta(2)-adrenergic drug in bovine hair has been developed, utilizing this oxidative response. After alkaline degradation of the hair, the drug was extracted using a simple liquid-liquid extraction based on hexane, with a recovery of 92 +/- 3.6%. After evaporation to dryness, the residue is dissolved in mobile phase and the drug analysed by reversed phase liquid chromatography using a mobile phase consisting of methanol-0.1 M ammonium acetate (60:40, v/v) with 0.4% sodium dodecyl sulphate adjusted to pH 5 with orthophosphoric acid. Quantitation used a two cell coulometric detector with the first cell being used as a screening electrode and the analytical electrode set at +875 mV.
    Original languageEnglish
    Pages (from-to)159-166
    JournalAnalytica Chimica Acta
    Volume322
    Issue number3
    Publication statusPublished - Apr 1996

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