Investigation of antibacterial phytochemicals in the bark and leaves of Ficus coronata by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn

Thomas J. P. Smyth, Venkataraman Ramachandran, Peter Brooks, Franklin Smyth

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The ethyl acetate extracts of the bark and leaves of Ficus coronata were separated by column chromatography and the resulting fractions tested for their bioactivity toward methicillin-resistant-Staphylococcus aureus (MRSA) and M. luteus. The bioactive column chromatography fractions were further separated by preparative thin layer chromatography (TLC) and the resulting bands investigated by high-performance liquid chromatographyelectrospray ionizationion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn. The resulting retention times, molecular masses, their fragmentation patterns, and the chemnet database () were then used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations were found that did not correlate with any known compounds thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multidrug-resistant microorganisms. Structures are also proposed for known compounds that have not been previously reported for F. coronata.
    LanguageEnglish
    Pages713-718
    JournalElectrophoresis
    Volume33
    Issue number4, SI
    DOIs
    Publication statusPublished - Feb 2012

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    Electrospray ionization
    Column chromatography
    Methicillin
    High performance liquid chromatography
    Phytochemicals
    Molecular mass
    Mass spectrometry
    Ions
    Thin layer chromatography
    Bioactivity
    Biological Products
    Microorganisms
    Liquids
    Pharmaceutical Preparations
    ethyl acetate

    Cite this

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    title = "Investigation of antibacterial phytochemicals in the bark and leaves of Ficus coronata by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn",
    abstract = "The ethyl acetate extracts of the bark and leaves of Ficus coronata were separated by column chromatography and the resulting fractions tested for their bioactivity toward methicillin-resistant-Staphylococcus aureus (MRSA) and M. luteus. The bioactive column chromatography fractions were further separated by preparative thin layer chromatography (TLC) and the resulting bands investigated by high-performance liquid chromatographyelectrospray ionizationion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn. The resulting retention times, molecular masses, their fragmentation patterns, and the chemnet database () were then used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations were found that did not correlate with any known compounds thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multidrug-resistant microorganisms. Structures are also proposed for known compounds that have not been previously reported for F. coronata.",
    author = "Smyth, {Thomas J. P.} and Venkataraman Ramachandran and Peter Brooks and Franklin Smyth",
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    Investigation of antibacterial phytochemicals in the bark and leaves of Ficus coronata by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn. / Smyth, Thomas J. P.; Ramachandran, Venkataraman; Brooks, Peter; Smyth, Franklin.

    In: Electrophoresis, Vol. 33, No. 4, SI, 02.2012, p. 713-718.

    Research output: Contribution to journalArticle

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    AB - The ethyl acetate extracts of the bark and leaves of Ficus coronata were separated by column chromatography and the resulting fractions tested for their bioactivity toward methicillin-resistant-Staphylococcus aureus (MRSA) and M. luteus. The bioactive column chromatography fractions were further separated by preparative thin layer chromatography (TLC) and the resulting bands investigated by high-performance liquid chromatographyelectrospray ionizationion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn. The resulting retention times, molecular masses, their fragmentation patterns, and the chemnet database () were then used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations were found that did not correlate with any known compounds thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multidrug-resistant microorganisms. Structures are also proposed for known compounds that have not been previously reported for F. coronata.

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