Investigation of Animal Venoms and Toxins

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter contains sections titled:* Introduction* Natural Bioresources* Pharmaceutical Success Stories* The Methods in Toxin Sequencing* Reducing Complexity by Chromatography* Bioassay Considerations* Classical Edman* Overview of MS Methods for Chemical Prospecting* Soft Ionization Methods* Common Mass Analyzers for Peptide Analysis* Information Obtained from Different MS Systems* Cloning Studies* New Developments and Future Trends* Conclusions* Other Sources of Information* References
Original languageEnglish
Title of host publicationMethods in Animal Proteomics
PublisherJohn Wiley & Sons, Inc.
Pages211-241
ISBN (Print)978-0813817910
DOIs
Publication statusPublished - 2011

Keywords

  • animal venom and toxin investigation
  • mass spectrometric technologies in structural characterization of peptides
  • role of matrix-assisted laser desorption/ionization-time-of-flight (MALDI
  • TOF) analysis
  • tandem mass spectrometry (MS/MS) sequencing combined with database searching
  • Escoubas and colleagues
  • and therapeutic potential of peptides
  • from arachnids
  • pharmaceutical success stories
  • Cytolex (Pexiganan)
  • in foot ulcer treatment
  • in diabetic patients
  • toxin sequencing methods
  • first step in analytical process
  • venom from specimens
  • novel approach by Tyler's group in Australia
  • transdermal electrical stimulation for skin secretion extraction
  • reducing complexity by chromatography
  • skin secretions
  • by separation technologies
  • MS methods for chemical prospecting
  • standard tool in laboratories
  • MS/MS Methods MS/MS sequencing
  • and database searching
  • identifying previously characterized sequences
  • method of corroborating sequence calls
  • needed
  • de novo sequencing
  • and Edman degradation or by cDNA cloning studies

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  • Cite this

    McClean, S. (2011). Investigation of Animal Venoms and Toxins. In Methods in Animal Proteomics (pp. 211-241). John Wiley & Sons, Inc.. https://doi.org/10.1002/9780470960660.ch9