Abstract
This chapter contains sections titled:* Introduction* Natural Bioresources* Pharmaceutical Success Stories* The Methods in Toxin Sequencing* Reducing Complexity by Chromatography* Bioassay Considerations* Classical Edman* Overview of MS Methods for Chemical Prospecting* Soft Ionization Methods* Common Mass Analyzers for Peptide Analysis* Information Obtained from Different MS Systems* Cloning Studies* New Developments and Future Trends* Conclusions* Other Sources of Information* References
Original language | English |
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Title of host publication | Methods in Animal Proteomics |
Publisher | John Wiley & Sons, Inc. |
Pages | 211-241 |
ISBN (Print) | 978-0813817910 |
DOIs | |
Publication status | Published (in print/issue) - 2011 |
Keywords
- animal venom and toxin investigation
- mass spectrometric technologies in structural characterization of peptides
- role of matrix-assisted laser desorption/ionization-time-of-flight (MALDI
- TOF) analysis
- tandem mass spectrometry (MS/MS) sequencing combined with database searching
- Escoubas and colleagues
- and therapeutic potential of peptides
- from arachnids
- pharmaceutical success stories
- Cytolex (Pexiganan)
- in foot ulcer treatment
- in diabetic patients
- toxin sequencing methods
- first step in analytical process
- venom from specimens
- novel approach by Tyler's group in Australia
- transdermal electrical stimulation for skin secretion extraction
- reducing complexity by chromatography
- skin secretions
- by separation technologies
- MS methods for chemical prospecting
- standard tool in laboratories
- MS/MS Methods MS/MS sequencing
- and database searching
- identifying previously characterized sequences
- method of corroborating sequence calls
- needed
- de novo sequencing
- and Edman degradation or by cDNA cloning studies