INVESTIGATING THE LINK BETWEEN HYPOXIA AND MIR-21 IN PROSTATE CANCER.

Research output: Contribution to journalMeeting Abstract

Abstract

Background: Tumour hypoxia is a major driver of prostate cancer
progression and metastasis. miR-21 is a microRNA which has been
previously linked to hypoxia, but this relationship remains poorly
characterised in a prostate cancer setting. Therefore, in this study,
we investigate the link between hypoxia and miR-21 in prostate
cancer cells.
Methods: We have used 2D and 3D cell prostate cell models of
hypoxia to investigate the functionality of miR-21. Expression levels
of miR-21 have been measured by qPCR and functional bioassays
used to examine its effect on prostate cell behaviour. Target genes
have been identified and bioinformatic analysis has been employed
to investigate a clinical significance for miR-21 in prostate cancer.
Results: miR-21 is induced by hypoxia in prostate cancer cell-lines.
Over-expression of miR-21 impacts upon target genes which in
turn affects cell behaviour. Data-mining of online repositories of
clinical data and bioinformatic analysis of miR-21 cellular networks
reveal that miR-21 exerts a wide influence on several important cell
processes, the dysregulation of which can lead to development of
prostate cancer.
Conclusions: We propose that miR-21 could be an important
microRNA in the pathogenesis of prostate cancer and has potential
as a biomarker in this disease.
LanguageEnglish
Pages66
JournalUlster Medical Journal, The
Volume88
Issue number1
Publication statusPublished - Jan 2019

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Prostatic Neoplasms
Prostate
Computational Biology
Data Mining
MicroRNAs
Biomarkers
Hypoxia
Neoplasm Metastasis
Cell Line
Genes
Neoplasms

Cite this

@article{3c5b81f74fa7428cae124088be5a00fb,
title = "INVESTIGATING THE LINK BETWEEN HYPOXIA AND MIR-21 IN PROSTATE CANCER.",
abstract = "Background: Tumour hypoxia is a major driver of prostate cancerprogression and metastasis. miR-21 is a microRNA which has beenpreviously linked to hypoxia, but this relationship remains poorlycharacterised in a prostate cancer setting. Therefore, in this study,we investigate the link between hypoxia and miR-21 in prostatecancer cells.Methods: We have used 2D and 3D cell prostate cell models ofhypoxia to investigate the functionality of miR-21. Expression levelsof miR-21 have been measured by qPCR and functional bioassaysused to examine its effect on prostate cell behaviour. Target geneshave been identified and bioinformatic analysis has been employedto investigate a clinical significance for miR-21 in prostate cancer.Results: miR-21 is induced by hypoxia in prostate cancer cell-lines.Over-expression of miR-21 impacts upon target genes which inturn affects cell behaviour. Data-mining of online repositories ofclinical data and bioinformatic analysis of miR-21 cellular networksreveal that miR-21 exerts a wide influence on several important cellprocesses, the dysregulation of which can lead to development ofprostate cancer.Conclusions: We propose that miR-21 could be an importantmicroRNA in the pathogenesis of prostate cancer and has potentialas a biomarker in this disease.",
author = "Zoe Angel and CP Walsh and McKenna, {Declan J}",
year = "2019",
month = "1",
language = "English",
volume = "88",
pages = "66",
journal = "Ulster Medical Journal, The",
issn = "0041-6193",
number = "1",

}

INVESTIGATING THE LINK BETWEEN HYPOXIA AND MIR-21 IN PROSTATE CANCER. / Angel, Zoe; Walsh, CP; McKenna, Declan J.

In: Ulster Medical Journal, The, Vol. 88, No. 1, 01.2019, p. 66.

Research output: Contribution to journalMeeting Abstract

TY - JOUR

T1 - INVESTIGATING THE LINK BETWEEN HYPOXIA AND MIR-21 IN PROSTATE CANCER.

AU - Angel, Zoe

AU - Walsh, CP

AU - McKenna, Declan J

PY - 2019/1

Y1 - 2019/1

N2 - Background: Tumour hypoxia is a major driver of prostate cancerprogression and metastasis. miR-21 is a microRNA which has beenpreviously linked to hypoxia, but this relationship remains poorlycharacterised in a prostate cancer setting. Therefore, in this study,we investigate the link between hypoxia and miR-21 in prostatecancer cells.Methods: We have used 2D and 3D cell prostate cell models ofhypoxia to investigate the functionality of miR-21. Expression levelsof miR-21 have been measured by qPCR and functional bioassaysused to examine its effect on prostate cell behaviour. Target geneshave been identified and bioinformatic analysis has been employedto investigate a clinical significance for miR-21 in prostate cancer.Results: miR-21 is induced by hypoxia in prostate cancer cell-lines.Over-expression of miR-21 impacts upon target genes which inturn affects cell behaviour. Data-mining of online repositories ofclinical data and bioinformatic analysis of miR-21 cellular networksreveal that miR-21 exerts a wide influence on several important cellprocesses, the dysregulation of which can lead to development ofprostate cancer.Conclusions: We propose that miR-21 could be an importantmicroRNA in the pathogenesis of prostate cancer and has potentialas a biomarker in this disease.

AB - Background: Tumour hypoxia is a major driver of prostate cancerprogression and metastasis. miR-21 is a microRNA which has beenpreviously linked to hypoxia, but this relationship remains poorlycharacterised in a prostate cancer setting. Therefore, in this study,we investigate the link between hypoxia and miR-21 in prostatecancer cells.Methods: We have used 2D and 3D cell prostate cell models ofhypoxia to investigate the functionality of miR-21. Expression levelsof miR-21 have been measured by qPCR and functional bioassaysused to examine its effect on prostate cell behaviour. Target geneshave been identified and bioinformatic analysis has been employedto investigate a clinical significance for miR-21 in prostate cancer.Results: miR-21 is induced by hypoxia in prostate cancer cell-lines.Over-expression of miR-21 impacts upon target genes which inturn affects cell behaviour. Data-mining of online repositories ofclinical data and bioinformatic analysis of miR-21 cellular networksreveal that miR-21 exerts a wide influence on several important cellprocesses, the dysregulation of which can lead to development ofprostate cancer.Conclusions: We propose that miR-21 could be an importantmicroRNA in the pathogenesis of prostate cancer and has potentialas a biomarker in this disease.

UR - https://www.ums.ac.uk/umj088/088(1)062.pdf

M3 - Meeting Abstract

VL - 88

SP - 66

JO - Ulster Medical Journal, The

T2 - Ulster Medical Journal, The

JF - Ulster Medical Journal, The

SN - 0041-6193

IS - 1

ER -