Investigating the Effects of Fluid Shear Forces on Cellular Responses to Profiled Surfaces in-Vitro: A Computational and Experimental Investigation

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

10 Citations (Scopus)


The flow conditions in a parallel plate “bioreactor” have been modeled using Computational Fluid Dynamics (CFD) and characterized experimentally using acomputer controlled flow regulation apparatus and associated flow visualization techniques. The conditions required to induce flow characteristics appropriate for standard in-vitro cell processes on flat substrates have been identified from a consideration of data from previous studies. The effects of changing the surface topography of the substrate on which the cells are grown has been investigated by forming specific micrometer scale features via hot embossing on polymeric plates. The effect that various features have on the flow that occurs at the boundary layer adjacent to the surface in the parallel plate chamber has been determined. The results for studies undertaken in a parallel platechamber operating under computer control are presented here. Flow characteristics have been determined using feedback from the sensors within the system and validated by direct flow visualization using fluorescent beads and modeled using a CFD routine. The system exhibits well developed laminar flow and is capable of delivering surface shear stresses up to 2.4 Pa on a planar surface. As such, it is suitable for evaluating in-vitro cellprocesses. The effects that the features produced by hot embossing of a Poly Methyl Methacrylate (PMMA) surface have been tested in the flow chamber and their influence onshear stress observed.
Original languageEnglish
Title of host publicationUnknown Host Publication
Number of pages4
Publication statusPublished (in print/issue) - 23 Aug 2007
Event29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Lyon, France
Duration: 23 Aug 2007 → …


Conference29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Period23/08/07 → …

Bibliographical note

Reference text: [1] J. Klein-Nulend, A. van der Plas, C. M. Semeins, N. E. Ajubi, J. A. Frangos, P. J. Nijweide and E. H. Burger, "Sensitivity of osteocytes to biomechanical stress in vitro," The FASEB Journal : Official Publication of the Federation of American Societies for. Experimental
Biology, vol. 9, pp. 441-445, Mar. 1995.

[2] I. Martin, D. Wendt and M. Heberer, "The role of bioreactors in tissue engineering," Trends in. Biotechnology, vol. 22, pp. 80-86, Feb. 2004.

[3] P. J. ter Brugge and J. A. Jansen, "Initial interaction of rat bone marrow cells with non-coated and calcium phosphate coated titanium substrates," Biomaterials, vol. 23, pp. 3269-3277, 2002/8.

[4] B. D. Boyan, L. F. Bonewald, E. P. Paschalis, C. H. Lohmann, J. Rosser, D. L. Cochran, D. D. Dean, Z. Schwartz and A. L. Boskey, "Osteoblast-mediated mineral deposition in culture is dependent on surface microtopography," Calcified Tissue International, vol. 71, pp. 519-529, Dec. 2002.

[5] S. R. Bannister, C. H. Lohmann, Y. Liu, V. L. Sylvia, D. L. Cochran, D. D. Dean, B. D. Boyan and Z. Schwartz, "Shear force modulates osteoblast response to surface roughness," Journal of Biomedical Materials Research, vol. 60, pp. 167-174, Apr. 2002.

[6] Z. Schwartz, T.A. Dennison, S.R. Bannister, D.L. Cochran, Y.H. Liu, M. Wieland, B.D. Boyan. “Osteoblast response to fluid induced shear depends on substrate microarchitecture and varies with time.”, Journal of Biomedical Materials Research Part A, in press.

[7] J. A. Frangos, L. V. McIntire and S. G. Eskin, "Shear stress induced stimulation of mammalian cell metabolism," Biotechnol. Bioeng., vol. 32, pp. 1053-1060, 1988.

[8] E. A. Nauman, K. J. Risic, T. M. Keaveny and R. L. Satcher, "Quantitative assessment of steady and pulsatile flow fields in a parallel plate flow chamber," Annals of Biomedical Engineering, vol. 27, pp. 194-199, Mar-Apr. 1999.

[9] E. J. Anderson, T. D. Falls, A. M. Sorkin and M. L. K. Tate, "The imperative for controlled mechanical stresses in unraveling cellular mechanisms of mechanotransduction," Biomedical Engineering Online [Electronic. Resource], vol. 5, pp. 27, 2006.

[10] F. Horgan and B. J. Meenan, "Effects of Substrate Morphology on Osteoblastic Response to Thin Film Calcium Phosphate Coatings," Key Engineering Materials, vol. 240 - 242, pp. 433-436, 2003.

[11] D. Sinton, "Microscale flow visualization," Microfluidics and Nanofluidics, vol. V1, pp. 2-21, 11/01/. 2004. G. O. Young, “Synthetic structure of industrial plastics (Book style with paper title and editor),” in Plastics, 2nd ed. vol. 3, J. Peters, Ed. New York:
McGraw-Hill, 1964, pp. 15–64.

[12] M. Nakamura, M. Kobayashi, N. Kuzuya, T. Komatsu and T, Mochizuka, “Hydrophilic
property of SiO2/TiO2 double layer films.” Thin Solid Films, vol 502, pp121-124, 2006


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