Intraobserver and Interobserver Reproducibility of CINtec® PLUS in ThinPrep® Cytology Preparations

Mary McMenamin, Michael McKenna, Andrew McDowell, Dawson Catriona, McKenna Roisin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BACKGROUND: This study evaluated the intraobserver and interobserver reproducibility of the dual-stain biomarker, CINtec® PLUS cytology in ThinPrep specimens, for improved specificity in the detection of cervical disease in women testing HPV positive. METHODS: A total of 972 cases of HPV positive women from a triage and primary HPV screening population were selected from an ongoing study evaluating the clinical performance of CINtec® PLUS cytology. For reproducibility analyses, three cytotechnologists rescreened sets of slides which they had previously reported themselves and which were previously reported by each of the other cytotechnologists. The original results of slides previously screened by each of the three cytotechnologists were also compared with the results of an expert reference evaluator. RESULTS: Intraobserver and interobserver agreement for paired evaluations between reviewers ranged from 82.8% to 94.9% (kappa 0.65-0.91) and 89.2% to 93% (kappa 0.83-0.88) respectively. Reproducibility analyses between the cytotechnologists and the reference evaluator revealed agreements ranging from 95.5% to 98% (kappa 0.89- 0.96). CONCLUSION: Evaluation of the dual-stain biomarker showed a high level of agreement across all evaluators suggesting that CINtec® PLUS cytology will perform well in the hands of cytotechnologists and pathologist reviewers and could be introduced into cellular pathology laboratories that employ ThinPrep LBC with minimum effort.
Original languageEnglish
Pages (from-to)1-20
JournalCytopathology
VolumeTBC
Early online date6 Jul 2017
DOIs
Publication statusE-pub ahead of print - 6 Jul 2017

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Cell Biology
Coloring Agents
Biomarkers
Triage
Pathology
Population
Pathologists
Clinical Studies

Keywords

  • Intraobserver
  • Interobserver
  • Reproducibility
  • CINtec® PLUS
  • ThinPrep® Cytology Preparations

Cite this

McMenamin, Mary ; McKenna, Michael ; McDowell, Andrew ; Catriona, Dawson ; Roisin, McKenna. / Intraobserver and Interobserver Reproducibility of CINtec® PLUS in ThinPrep® Cytology Preparations. In: Cytopathology. 2017 ; Vol. TBC. pp. 1-20.
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abstract = "BACKGROUND: This study evaluated the intraobserver and interobserver reproducibility of the dual-stain biomarker, CINtec{\circledR} PLUS cytology in ThinPrep specimens, for improved specificity in the detection of cervical disease in women testing HPV positive. METHODS: A total of 972 cases of HPV positive women from a triage and primary HPV screening population were selected from an ongoing study evaluating the clinical performance of CINtec{\circledR} PLUS cytology. For reproducibility analyses, three cytotechnologists rescreened sets of slides which they had previously reported themselves and which were previously reported by each of the other cytotechnologists. The original results of slides previously screened by each of the three cytotechnologists were also compared with the results of an expert reference evaluator. RESULTS: Intraobserver and interobserver agreement for paired evaluations between reviewers ranged from 82.8{\%} to 94.9{\%} (kappa 0.65-0.91) and 89.2{\%} to 93{\%} (kappa 0.83-0.88) respectively. Reproducibility analyses between the cytotechnologists and the reference evaluator revealed agreements ranging from 95.5{\%} to 98{\%} (kappa 0.89- 0.96). CONCLUSION: Evaluation of the dual-stain biomarker showed a high level of agreement across all evaluators suggesting that CINtec{\circledR} PLUS cytology will perform well in the hands of cytotechnologists and pathologist reviewers and could be introduced into cellular pathology laboratories that employ ThinPrep LBC with minimum effort.",
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Intraobserver and Interobserver Reproducibility of CINtec® PLUS in ThinPrep® Cytology Preparations. / McMenamin, Mary; McKenna, Michael; McDowell, Andrew; Catriona, Dawson; Roisin, McKenna.

In: Cytopathology, Vol. TBC, 06.07.2017, p. 1-20.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Intraobserver and Interobserver Reproducibility of CINtec® PLUS in ThinPrep® Cytology Preparations

AU - McMenamin, Mary

AU - McKenna, Michael

AU - McDowell, Andrew

AU - Catriona, Dawson

AU - Roisin, McKenna

PY - 2017/7/6

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N2 - BACKGROUND: This study evaluated the intraobserver and interobserver reproducibility of the dual-stain biomarker, CINtec® PLUS cytology in ThinPrep specimens, for improved specificity in the detection of cervical disease in women testing HPV positive. METHODS: A total of 972 cases of HPV positive women from a triage and primary HPV screening population were selected from an ongoing study evaluating the clinical performance of CINtec® PLUS cytology. For reproducibility analyses, three cytotechnologists rescreened sets of slides which they had previously reported themselves and which were previously reported by each of the other cytotechnologists. The original results of slides previously screened by each of the three cytotechnologists were also compared with the results of an expert reference evaluator. RESULTS: Intraobserver and interobserver agreement for paired evaluations between reviewers ranged from 82.8% to 94.9% (kappa 0.65-0.91) and 89.2% to 93% (kappa 0.83-0.88) respectively. Reproducibility analyses between the cytotechnologists and the reference evaluator revealed agreements ranging from 95.5% to 98% (kappa 0.89- 0.96). CONCLUSION: Evaluation of the dual-stain biomarker showed a high level of agreement across all evaluators suggesting that CINtec® PLUS cytology will perform well in the hands of cytotechnologists and pathologist reviewers and could be introduced into cellular pathology laboratories that employ ThinPrep LBC with minimum effort.

AB - BACKGROUND: This study evaluated the intraobserver and interobserver reproducibility of the dual-stain biomarker, CINtec® PLUS cytology in ThinPrep specimens, for improved specificity in the detection of cervical disease in women testing HPV positive. METHODS: A total of 972 cases of HPV positive women from a triage and primary HPV screening population were selected from an ongoing study evaluating the clinical performance of CINtec® PLUS cytology. For reproducibility analyses, three cytotechnologists rescreened sets of slides which they had previously reported themselves and which were previously reported by each of the other cytotechnologists. The original results of slides previously screened by each of the three cytotechnologists were also compared with the results of an expert reference evaluator. RESULTS: Intraobserver and interobserver agreement for paired evaluations between reviewers ranged from 82.8% to 94.9% (kappa 0.65-0.91) and 89.2% to 93% (kappa 0.83-0.88) respectively. Reproducibility analyses between the cytotechnologists and the reference evaluator revealed agreements ranging from 95.5% to 98% (kappa 0.89- 0.96). CONCLUSION: Evaluation of the dual-stain biomarker showed a high level of agreement across all evaluators suggesting that CINtec® PLUS cytology will perform well in the hands of cytotechnologists and pathologist reviewers and could be introduced into cellular pathology laboratories that employ ThinPrep LBC with minimum effort.

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KW - Interobserver

KW - Reproducibility

KW - CINtec® PLUS

KW - ThinPrep® Cytology Preparations

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DO - 10.1111/cyt.12426

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C2 - 28685883

VL - TBC

SP - 1

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JO - Cytopathology

JF - Cytopathology

SN - 0956-5507

ER -