Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer

Mandeep Marwah, Yvonne Perrie, Raj K. Singh Badhan, Deborah Lowry

Research output: Contribution to journalArticle

Abstract

Caucasian population groups have a higher propensity to develop skin cancer, and associated clinical interventions often present substantial financial burden on healthcare services. Conventional treatments are often not suitable for all patient groups as a result of poor efficacy and toxicity profiles. The primary objective of this study was to develop a deformable liposomal formulation, the properties of which being dictated by the surfactant Tween 20, for the dermal cellular delivery of epigallocatechin gallatein (EGCG), a compound possessing antineoplastic properties. The results demonstrated a significant (p ≤0.05) decrease in liposome deformability index (74 ± 8 to 37 ± 7) as Tween 20 loading increased from 0 to 10 % w/w, indicating an increase in elasticity. EGCG release over 24-hours demonstrated Tween 20 incorporation directly increased release from 13.7 % ± 1.1 % to 94.4 % ± 4.9 % (for 0 and 10 % w/w Tween 20 respectively). Finally, we demonstrated DilC-loaded deformable liposomes were localised intracellularly within human dermal fibroblast and keratinocyte cells within 2-hours. Thus it was evident deformable liposomes may aid drug penetration into dermal cells and would be useful in developing a controlled-release formulation.
LanguageEnglish
JournalJournal of Liposome Research
Publication statusAccepted/In press - 18 Mar 2019

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Polysorbates
Skin Neoplasms
Liposomes
Skin
Elasticity
Keratinocytes
Population Groups
Surface-Active Agents
Antineoplastic Agents
Fibroblasts
Delivery of Health Care
gallocatechol
Pharmaceutical Preparations
Therapeutics

Keywords

  • Skin cancer
  • deformable liposomes
  • dermal release
  • controlled release
  • elastic liposomes

Cite this

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title = "Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer",
abstract = "Caucasian population groups have a higher propensity to develop skin cancer, and associated clinical interventions often present substantial financial burden on healthcare services. Conventional treatments are often not suitable for all patient groups as a result of poor efficacy and toxicity profiles. The primary objective of this study was to develop a deformable liposomal formulation, the properties of which being dictated by the surfactant Tween 20, for the dermal cellular delivery of epigallocatechin gallatein (EGCG), a compound possessing antineoplastic properties. The results demonstrated a significant (p ≤0.05) decrease in liposome deformability index (74 ± 8 to 37 ± 7) as Tween 20 loading increased from 0 to 10 {\%} w/w, indicating an increase in elasticity. EGCG release over 24-hours demonstrated Tween 20 incorporation directly increased release from 13.7 {\%} ± 1.1 {\%} to 94.4 {\%} ± 4.9 {\%} (for 0 and 10 {\%} w/w Tween 20 respectively). Finally, we demonstrated DilC-loaded deformable liposomes were localised intracellularly within human dermal fibroblast and keratinocyte cells within 2-hours. Thus it was evident deformable liposomes may aid drug penetration into dermal cells and would be useful in developing a controlled-release formulation.",
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author = "Mandeep Marwah and Yvonne Perrie and Badhan, {Raj K. Singh} and Deborah Lowry",
year = "2019",
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Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer. / Marwah, Mandeep ; Perrie, Yvonne; Badhan, Raj K. Singh; Lowry, Deborah.

In: Journal of Liposome Research, 18.03.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer

AU - Marwah, Mandeep

AU - Perrie, Yvonne

AU - Badhan, Raj K. Singh

AU - Lowry, Deborah

PY - 2019/3/18

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N2 - Caucasian population groups have a higher propensity to develop skin cancer, and associated clinical interventions often present substantial financial burden on healthcare services. Conventional treatments are often not suitable for all patient groups as a result of poor efficacy and toxicity profiles. The primary objective of this study was to develop a deformable liposomal formulation, the properties of which being dictated by the surfactant Tween 20, for the dermal cellular delivery of epigallocatechin gallatein (EGCG), a compound possessing antineoplastic properties. The results demonstrated a significant (p ≤0.05) decrease in liposome deformability index (74 ± 8 to 37 ± 7) as Tween 20 loading increased from 0 to 10 % w/w, indicating an increase in elasticity. EGCG release over 24-hours demonstrated Tween 20 incorporation directly increased release from 13.7 % ± 1.1 % to 94.4 % ± 4.9 % (for 0 and 10 % w/w Tween 20 respectively). Finally, we demonstrated DilC-loaded deformable liposomes were localised intracellularly within human dermal fibroblast and keratinocyte cells within 2-hours. Thus it was evident deformable liposomes may aid drug penetration into dermal cells and would be useful in developing a controlled-release formulation.

AB - Caucasian population groups have a higher propensity to develop skin cancer, and associated clinical interventions often present substantial financial burden on healthcare services. Conventional treatments are often not suitable for all patient groups as a result of poor efficacy and toxicity profiles. The primary objective of this study was to develop a deformable liposomal formulation, the properties of which being dictated by the surfactant Tween 20, for the dermal cellular delivery of epigallocatechin gallatein (EGCG), a compound possessing antineoplastic properties. The results demonstrated a significant (p ≤0.05) decrease in liposome deformability index (74 ± 8 to 37 ± 7) as Tween 20 loading increased from 0 to 10 % w/w, indicating an increase in elasticity. EGCG release over 24-hours demonstrated Tween 20 incorporation directly increased release from 13.7 % ± 1.1 % to 94.4 % ± 4.9 % (for 0 and 10 % w/w Tween 20 respectively). Finally, we demonstrated DilC-loaded deformable liposomes were localised intracellularly within human dermal fibroblast and keratinocyte cells within 2-hours. Thus it was evident deformable liposomes may aid drug penetration into dermal cells and would be useful in developing a controlled-release formulation.

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KW - deformable liposomes

KW - dermal release

KW - controlled release

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