Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer

Mandeep Marwah, Yvonne Perrie, Raj K. Singh Badhan, Deborah Lowry

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Abstract

Caucasian population groups have a higher propensity to develop skin cancer, and associated clinical interventions often present substantial financial burden on healthcare services. Conventional treatments are often not suitable for all patient groups as a result of poor efficacy and toxicity profiles. The primary objective of this study was to develop a deformable liposomal formulation, the properties of which being dictated by the surfactant Tween 20, for the dermal cellular delivery of epigallocatechin gallatein (EGCG), a compound possessing antineoplastic properties. The results demonstrated a significant (p ≤0.05) decrease in liposome deformability index (74 ± 8 to 37 ± 7) as Tween 20 loading increased from 0 to 10 % w/w, indicating an increase in elasticity. EGCG release over 24-hours demonstrated Tween 20 incorporation directly increased release from 13.7 % ± 1.1 % to 94.4 % ± 4.9 % (for 0 and 10 % w/w Tween 20 respectively). Finally, we demonstrated DilC-loaded deformable liposomes were localised intracellularly within human dermal fibroblast and keratinocyte cells within 2-hours. Thus it was evident deformable liposomes may aid drug penetration into dermal cells and would be useful in developing a controlled-release formulation.
Original languageEnglish
JournalJournal of Liposome Research
Publication statusAccepted/In press - 18 Mar 2019

Keywords

  • Skin cancer
  • deformable liposomes
  • dermal release
  • controlled release
  • elastic liposomes

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