Insulin secretory actions of extracts of Asparagus racemosus root in perfused pancreas, isolated islets and clonal pancreatic beta-cells

J. M. A. Hannan, Lamin Marenah Liaquat Ali, Begurn Rokeya, Peter Flatt, Yasser Abdel-Wahab

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Asparagus racentosus root has previously been reported to reduce blood glucose in rats and rabbits. In the present study, the effects of the ethanol extract and five partition fractions of the root of A. racemosus were evaluated on insulin secretion together with exploration of their mechanisms of action. The ethanol extract and each of the hexane, chloroform and ethyl acetate partition fractions concentration-dependently stimulated insulin secretion in isolated perfused rat pancreas, isolated rat islet cells and clonal beta-cells. The stimulatory effects of the ethanol extract, hexane, chloroform and ethyl acetate partition fractions were potentiated by glucose, 3-isobutyl-1-methyl xanthine IBMX, tolbutamide and depolarizing concentration of KCl. Inhibition of A. racemosus-induced insulin release was observed with diazoxide and verapamil. Ethanol extract and five fractions increased intracellular Ca2+, consistent with the observed abolition of insulin secretory effects under Ca2+-free conditions. These findings reveal that constituents of A. racemosus root extracts have wide-ranging stimulatory effects on physiological insulinotropic pathways. Future work assessing the use of this plant as a source of active components may provide new opportunities for diabetes therapy.
LanguageEnglish
Pages159-168
JournalJournal of Endrocrinology
Volume192
Issue number1
DOIs
Publication statusPublished - Jan 2007

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Insulin-Secreting Cells
Pancreas
Ethanol
Insulin
Hexanes
Chloroform
Diazoxide
1-Methyl-3-isobutylxanthine
Tolbutamide
Xanthine
Verapamil
Islets of Langerhans
Blood Glucose
Rabbits
Glucose
ethyl acetate
Therapeutics

Cite this

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title = "Insulin secretory actions of extracts of Asparagus racemosus root in perfused pancreas, isolated islets and clonal pancreatic beta-cells",
abstract = "Asparagus racentosus root has previously been reported to reduce blood glucose in rats and rabbits. In the present study, the effects of the ethanol extract and five partition fractions of the root of A. racemosus were evaluated on insulin secretion together with exploration of their mechanisms of action. The ethanol extract and each of the hexane, chloroform and ethyl acetate partition fractions concentration-dependently stimulated insulin secretion in isolated perfused rat pancreas, isolated rat islet cells and clonal beta-cells. The stimulatory effects of the ethanol extract, hexane, chloroform and ethyl acetate partition fractions were potentiated by glucose, 3-isobutyl-1-methyl xanthine IBMX, tolbutamide and depolarizing concentration of KCl. Inhibition of A. racemosus-induced insulin release was observed with diazoxide and verapamil. Ethanol extract and five fractions increased intracellular Ca2+, consistent with the observed abolition of insulin secretory effects under Ca2+-free conditions. These findings reveal that constituents of A. racemosus root extracts have wide-ranging stimulatory effects on physiological insulinotropic pathways. Future work assessing the use of this plant as a source of active components may provide new opportunities for diabetes therapy.",
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Insulin secretory actions of extracts of Asparagus racemosus root in perfused pancreas, isolated islets and clonal pancreatic beta-cells. / Hannan, J. M. A.; Ali, Lamin Marenah Liaquat; Rokeya, Begurn; Flatt, Peter; Abdel-Wahab, Yasser.

In: Journal of Endrocrinology, Vol. 192, No. 1, 01.2007, p. 159-168.

Research output: Contribution to journalArticle

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