Inorganic and organic linker layers to aid immobilisation for SPR biosensing.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Improvements to the immobilisation of bio-recognition elements to sensor surfaces are keenly sought. Surface Plasmon Resonance is a highly sensitive optical based sensing technique that is being used in this research as a means of evaluating novel immobilisation techniques. We report on the establishment of binding-sites at the sensor surface using two diverse methods. In the first method, well established deposition techniques were used to coat the gold surface with a silicon rich matrix. It is demonstrated that control of the depth of the material to within 10 nm was achieved. In a second method highly ordered arrays of genetically modified biological materials have been used to form attachment sites and are being investigated. Careful choice of amino acid placement at the apical domain could provide bioselective attachment, with control in three dimensions in the region of 10's of nanometres. Characterisation of the active surfaces in each instance is presented using a number of well established techniques such as Scanning Electron Microscopy, Raman, Profilometry and Atomic Force Microscopy. Investigations, although at an early stage, have shown promise. Initial results obtained for sensitivity to glucose are indicative of an overall improvement over conventional techniques taking into account the key aspects of metal layer thickness and penetration depth of the surface plasmon wave.
Original languageEnglish
Title of host publicationUnknown Host Publication
Place of Publication1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
PublisherSPIE
Pages190-198
Number of pages9
Volume5824
DOIs
Publication statusPublished - 2005
EventOpto-Ireland 2005: Nanotechnology and Nanophotonics -
Duration: 1 Jan 2005 → …

Publication series

NamePROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)
PublisherSPIE-INT SOC OPTICAL ENGINEERING

Conference

ConferenceOpto-Ireland 2005: Nanotechnology and Nanophotonics
Period1/01/05 → …

Keywords

  • surface plasmon resonance
  • protein templates
  • immobilisation
  • linker layers biosensing

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  • Cite this

    Craig, I., & McLaughlin, JAD. (2005). Inorganic and organic linker layers to aid immobilisation for SPR biosensing. In Unknown Host Publication (Vol. 5824, pp. 190-198). (PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)). SPIE. https://doi.org/10.1117/12.607614