Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro

Yumi Z. H. -Y. Hashim, Ian R. Rowland, Hugh McGlynn, Maurizio Servili, Roberto Selvaggini, Agnese Taticchi, Sonia Esposto, GianFrancesco Montedoro, Leena Kaisalo, Kristiina Wahala, Chris Gill

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Studies in human, animal and cellular systems suggest that phenols from virgin olive oil are capable of inhibiting several stages in carcinogenesis, including metastasis. The invasion cascade comprises cell attachment to extracellular matrix components or basement membrane, degradation of basement membrane by proteolytic enzymes and migration of cells through the modified matrix. In the present study, we investigated the effect of phenolics extracted from virgin olive oil (OVP) and its main constituents: hydroxytyrosol (3,4-dihydroxyphenylethanol), tyrosol (p-hydroxyphenylethanol), pinoresinol and caffeic acid. The effects of these phenolics were tested on the invasion of HT115 human colon carcinoma cells in a Matrigel invasion assay. OVP and its compounds showed different dose-related anti-invasive effects. At 25 mu g/ml OVP and equivalent doses of individual compounds, significant anti-invasive effects were seen in the range of 45-55% of control. Importantly, OVP, but not the isolated phenolics, significantly reduced total cell number in the Matrigel invasion assay. There were no significant effects shown on cell viability, indicating the reduction of cell number in the Matrigel invasion assay was not due to cytotoxicity. There were also no significant effects on cell attachment to plastic substrate, indicating the importance of extracellular matrix in modulating the anti-invasive effects of OVP. In conclusion, the results from this study indicate that phenols from virgin olive oil have the ability to inhibit invasion of colon cancer cells and the effects may be mediated at different levels of the invasion cascade. (c) 2007 Wiley-Liss, Inc.
LanguageEnglish
Pages495-500
JournalInternational Journal of Cancer
Volume122
Issue number3
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Colon
Adenocarcinoma
Phenols
Basement Membrane
Extracellular Matrix
Cell Count
Colonic Neoplasms
Plastics
Cell Movement
Cell Survival
Carcinogenesis
Peptide Hydrolases
Neoplasm Metastasis
Carcinoma
Olive Oil
In Vitro Techniques
matrigel
4-hydroxyphenylethanol
3,4-dihydroxyphenylethanol

Cite this

Hashim, Y. Z. H. -Y., Rowland, I. R., McGlynn, H., Servili, M., Selvaggini, R., Taticchi, A., ... Gill, C. (2008). Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro. International Journal of Cancer, 122(3), 495-500. https://doi.org/10.1002/ijc.23148
Hashim, Yumi Z. H. -Y. ; Rowland, Ian R. ; McGlynn, Hugh ; Servili, Maurizio ; Selvaggini, Roberto ; Taticchi, Agnese ; Esposto, Sonia ; Montedoro, GianFrancesco ; Kaisalo, Leena ; Wahala, Kristiina ; Gill, Chris. / Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro. In: International Journal of Cancer. 2008 ; Vol. 122, No. 3. pp. 495-500.
@article{f344c3dd62dc4234b717d530db4bea95,
title = "Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro",
abstract = "Studies in human, animal and cellular systems suggest that phenols from virgin olive oil are capable of inhibiting several stages in carcinogenesis, including metastasis. The invasion cascade comprises cell attachment to extracellular matrix components or basement membrane, degradation of basement membrane by proteolytic enzymes and migration of cells through the modified matrix. In the present study, we investigated the effect of phenolics extracted from virgin olive oil (OVP) and its main constituents: hydroxytyrosol (3,4-dihydroxyphenylethanol), tyrosol (p-hydroxyphenylethanol), pinoresinol and caffeic acid. The effects of these phenolics were tested on the invasion of HT115 human colon carcinoma cells in a Matrigel invasion assay. OVP and its compounds showed different dose-related anti-invasive effects. At 25 mu g/ml OVP and equivalent doses of individual compounds, significant anti-invasive effects were seen in the range of 45-55{\%} of control. Importantly, OVP, but not the isolated phenolics, significantly reduced total cell number in the Matrigel invasion assay. There were no significant effects shown on cell viability, indicating the reduction of cell number in the Matrigel invasion assay was not due to cytotoxicity. There were also no significant effects on cell attachment to plastic substrate, indicating the importance of extracellular matrix in modulating the anti-invasive effects of OVP. In conclusion, the results from this study indicate that phenols from virgin olive oil have the ability to inhibit invasion of colon cancer cells and the effects may be mediated at different levels of the invasion cascade. (c) 2007 Wiley-Liss, Inc.",
author = "Hashim, {Yumi Z. H. -Y.} and Rowland, {Ian R.} and Hugh McGlynn and Maurizio Servili and Roberto Selvaggini and Agnese Taticchi and Sonia Esposto and GianFrancesco Montedoro and Leena Kaisalo and Kristiina Wahala and Chris Gill",
year = "2008",
month = "2",
doi = "10.1002/ijc.23148",
language = "English",
volume = "122",
pages = "495--500",
journal = "International Journal of Cancer",
issn = "0020-7136",
number = "3",

}

Hashim, YZH-Y, Rowland, IR, McGlynn, H, Servili, M, Selvaggini, R, Taticchi, A, Esposto, S, Montedoro, G, Kaisalo, L, Wahala, K & Gill, C 2008, 'Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro', International Journal of Cancer, vol. 122, no. 3, pp. 495-500. https://doi.org/10.1002/ijc.23148

Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro. / Hashim, Yumi Z. H. -Y.; Rowland, Ian R.; McGlynn, Hugh; Servili, Maurizio; Selvaggini, Roberto; Taticchi, Agnese; Esposto, Sonia; Montedoro, GianFrancesco; Kaisalo, Leena; Wahala, Kristiina; Gill, Chris.

In: International Journal of Cancer, Vol. 122, No. 3, 02.2008, p. 495-500.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro

AU - Hashim, Yumi Z. H. -Y.

AU - Rowland, Ian R.

AU - McGlynn, Hugh

AU - Servili, Maurizio

AU - Selvaggini, Roberto

AU - Taticchi, Agnese

AU - Esposto, Sonia

AU - Montedoro, GianFrancesco

AU - Kaisalo, Leena

AU - Wahala, Kristiina

AU - Gill, Chris

PY - 2008/2

Y1 - 2008/2

N2 - Studies in human, animal and cellular systems suggest that phenols from virgin olive oil are capable of inhibiting several stages in carcinogenesis, including metastasis. The invasion cascade comprises cell attachment to extracellular matrix components or basement membrane, degradation of basement membrane by proteolytic enzymes and migration of cells through the modified matrix. In the present study, we investigated the effect of phenolics extracted from virgin olive oil (OVP) and its main constituents: hydroxytyrosol (3,4-dihydroxyphenylethanol), tyrosol (p-hydroxyphenylethanol), pinoresinol and caffeic acid. The effects of these phenolics were tested on the invasion of HT115 human colon carcinoma cells in a Matrigel invasion assay. OVP and its compounds showed different dose-related anti-invasive effects. At 25 mu g/ml OVP and equivalent doses of individual compounds, significant anti-invasive effects were seen in the range of 45-55% of control. Importantly, OVP, but not the isolated phenolics, significantly reduced total cell number in the Matrigel invasion assay. There were no significant effects shown on cell viability, indicating the reduction of cell number in the Matrigel invasion assay was not due to cytotoxicity. There were also no significant effects on cell attachment to plastic substrate, indicating the importance of extracellular matrix in modulating the anti-invasive effects of OVP. In conclusion, the results from this study indicate that phenols from virgin olive oil have the ability to inhibit invasion of colon cancer cells and the effects may be mediated at different levels of the invasion cascade. (c) 2007 Wiley-Liss, Inc.

AB - Studies in human, animal and cellular systems suggest that phenols from virgin olive oil are capable of inhibiting several stages in carcinogenesis, including metastasis. The invasion cascade comprises cell attachment to extracellular matrix components or basement membrane, degradation of basement membrane by proteolytic enzymes and migration of cells through the modified matrix. In the present study, we investigated the effect of phenolics extracted from virgin olive oil (OVP) and its main constituents: hydroxytyrosol (3,4-dihydroxyphenylethanol), tyrosol (p-hydroxyphenylethanol), pinoresinol and caffeic acid. The effects of these phenolics were tested on the invasion of HT115 human colon carcinoma cells in a Matrigel invasion assay. OVP and its compounds showed different dose-related anti-invasive effects. At 25 mu g/ml OVP and equivalent doses of individual compounds, significant anti-invasive effects were seen in the range of 45-55% of control. Importantly, OVP, but not the isolated phenolics, significantly reduced total cell number in the Matrigel invasion assay. There were no significant effects shown on cell viability, indicating the reduction of cell number in the Matrigel invasion assay was not due to cytotoxicity. There were also no significant effects on cell attachment to plastic substrate, indicating the importance of extracellular matrix in modulating the anti-invasive effects of OVP. In conclusion, the results from this study indicate that phenols from virgin olive oil have the ability to inhibit invasion of colon cancer cells and the effects may be mediated at different levels of the invasion cascade. (c) 2007 Wiley-Liss, Inc.

U2 - 10.1002/ijc.23148

DO - 10.1002/ijc.23148

M3 - Article

VL - 122

SP - 495

EP - 500

JO - International Journal of Cancer

T2 - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 3

ER -

Hashim YZH-Y, Rowland IR, McGlynn H, Servili M, Selvaggini R, Taticchi A et al. Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro. International Journal of Cancer. 2008 Feb;122(3):495-500. https://doi.org/10.1002/ijc.23148