Aims Glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP) are important insulinotropic hormones that enhance the insulin secretory response to feeding. Their potential for treating Type 2 diabetes is limited by short biological half-life owing to degradation by dipeptidyl peptidase IV (DPP IV). We investigated the acute effects of metformin on DPP IV activity in Type 2 diabetes to elucidate inhibition of DPP IV as a possible mechanism of action. Methods Eight fasting subjects with Type 2 diabetes (5M/3F, age 53.1 +/- 4.2 years, BMI 36.8 +/- 1.8 kg/m(2), glucose 8.9 +/- 1.2 mmol/l, HbA(1c) 7.8 +/- 0.6%) received placebo or metformin 1 g orally 1 week apart in a random, crossover design. Results Following metformin, DPP IV activity was suppressed compared with placebo (AUC(0-6 h) 3230 +/- 373 vs. 5764 +/- 504 nmol ml/l, respectively, P = 0.001). Circulating glucose, insulin and total GLP-1 were unchanged. Metformin also concentration-dependently inhibited endogenous DPP IV activity in vitro in plasma from Type 2 diabetic subjects. Conclusion Oral metformin effectively inhibits DPP IV activity in Type 2 diabetic patients, suggesting that the drug may have potential for future combination therapy with incretin hormones.
|Publication status||Published - May 2005|
Lindsay, JR., Duffy, NA., McKillop, A., Ardill, J., O'Harte, F., Flatt, P., & Bell, PM. (2005). Inhibition of dipeptidyl peptidase IV activity by oral metformin in Type 2 diabetes. Diabetic medicine, 22(5), 654-657.