Inhibition of corrosion causing Pseudomonas aeruginosa using plasma-activated water

Eleni Asimakopoulou; Sotiriοs Ι.  Εkonomou; P Papakonstantinou; Olena  Doran; Alexandros Ch.  Stratakos

doi:10.1111/jam.15391

Inhibition of corrosion causing Pseudomonas aeruginosa using plasma-activated water

Eleni Asimakopoulou, Sotiriοs Ι. Εkonomou, P Papakonstantinou, Olena Doran, Alexandros Ch. Stratakos

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

335 Downloads (Pure)

Abstract

Aims: The cost of Microbiologically Influenced Corrosion (MIC) significantly affects a wide range of sectors. This study aims to assess the efficiency of a novel technology based on the use of plasma-activated water (PAW) in inhibiting corrosion caused by bacteria.

Methods and Results: This study evaluated the effectiveness of PAW, produced by a plasma bubble reactor, in reducing corrosion causing Pseudomonas aeruginosa planktonic cells in tap water and biofilms were grown onto stainless steel (SS) coupons. Planktonic cells and biofilms were treated with PAW at different discharge frequencies (500–1500 Hz) and exposure times (0–20 min). P. aeruginosa cells in tap water were significantly reduced after treatment, with higher exposure times and discharge frequencies achieving higher reductions. Also, PAW treatment led to a gradual reduction for young and mature biofilms, achieving >4-Log reductions after 20 min. Results were also used to develop two predictive inactivation models.

Conclusions: This work presents evidence that PAW can be used to inactivate both planktonic cells and biofilms of P. aeruginosa. Experimental and theoretical results also demonstrate that reduction is dependent on discharge frequency and exposure time.

Significance and Impact of the Study: This work demonstrates the potential of using PAW as means to control MIC.

Original language	English
Pages (from-to)	2781-2794
Number of pages	14
Journal	Journal of Applied Microbiology
Volume	132
Issue number	4
Early online date	30 Nov 2021
DOIs	https://doi.org/10.1111/jam.15391
Publication status	Published online - 30 Nov 2021

Bibliographical note

Funding Information:
This work was supported by the University of West of England ‐Bristol internal funding awarded to Alexandros Ch. Stratakos.

Publisher Copyright:
© 2021 The Society for Applied Microbiology

Keywords

plasma-activated water
biofilm
intracellular ATP levels
P. aeruginosa
microbial corrosion
Biotechnology
Applied Microbiology and Biotechnology
General Medicine
P
aeruginosa

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/jam.15391

jam.15391Author Accepted Manuscript, 13.7 MB

Cite this

@article{a17a8c83024a4a4b95bd9c945f882dcd,

title = "Inhibition of corrosion causing Pseudomonas aeruginosa using plasma-activated water",

abstract = "Aims: The cost of Microbiologically Influenced Corrosion (MIC) significantly affects a wide range of sectors. This study aims to assess the efficiency of a novel technology based on the use of plasma-activated water (PAW) in inhibiting corrosion caused by bacteria. Methods and Results: This study evaluated the effectiveness of PAW, produced by a plasma bubble reactor, in reducing corrosion causing Pseudomonas aeruginosa planktonic cells in tap water and biofilms were grown onto stainless steel (SS) coupons. Planktonic cells and biofilms were treated with PAW at different discharge frequencies (500–1500 Hz) and exposure times (0–20 min). P. aeruginosa cells in tap water were significantly reduced after treatment, with higher exposure times and discharge frequencies achieving higher reductions. Also, PAW treatment led to a gradual reduction for young and mature biofilms, achieving >4-Log reductions after 20 min. Results were also used to develop two predictive inactivation models. Conclusions: This work presents evidence that PAW can be used to inactivate both planktonic cells and biofilms of P. aeruginosa. Experimental and theoretical results also demonstrate that reduction is dependent on discharge frequency and exposure time. Significance and Impact of the Study: This work demonstrates the potential of using PAW as means to control MIC.",

keywords = "plasma-activated water, biofilm, intracellular ATP levels, P. aeruginosa, microbial corrosion, Biotechnology, Applied Microbiology and Biotechnology, General Medicine, P, aeruginosa",

author = "Eleni Asimakopoulou and Εkonomou, {Sotiriοs Ι.} and P Papakonstantinou and Olena Doran and Stratakos, {Alexandros Ch.}",

note = "Funding Information: This work was supported by the University of West of England ‐Bristol internal funding awarded to Alexandros Ch. Stratakos. Publisher Copyright: {\textcopyright} 2021 The Society for Applied Microbiology",

year = "2021",

month = nov,

day = "30",

doi = "10.1111/jam.15391",

language = "English",

volume = "132",

pages = "2781--2794",

journal = "Journal of Applied Microbiology",

issn = "1364-5072",

publisher = "John Wiley & Sons, Inc.",

number = "4",

}

TY - JOUR

T1 - Inhibition of corrosion causing Pseudomonas aeruginosa using plasma-activated water

AU - Asimakopoulou, Eleni

AU - Εkonomou, Sotiriοs Ι.

AU - Papakonstantinou, P

AU - Doran, Olena

AU - Stratakos, Alexandros Ch.

N1 - Funding Information: This work was supported by the University of West of England ‐Bristol internal funding awarded to Alexandros Ch. Stratakos. Publisher Copyright: © 2021 The Society for Applied Microbiology

PY - 2021/11/30

Y1 - 2021/11/30

N2 - Aims: The cost of Microbiologically Influenced Corrosion (MIC) significantly affects a wide range of sectors. This study aims to assess the efficiency of a novel technology based on the use of plasma-activated water (PAW) in inhibiting corrosion caused by bacteria. Methods and Results: This study evaluated the effectiveness of PAW, produced by a plasma bubble reactor, in reducing corrosion causing Pseudomonas aeruginosa planktonic cells in tap water and biofilms were grown onto stainless steel (SS) coupons. Planktonic cells and biofilms were treated with PAW at different discharge frequencies (500–1500 Hz) and exposure times (0–20 min). P. aeruginosa cells in tap water were significantly reduced after treatment, with higher exposure times and discharge frequencies achieving higher reductions. Also, PAW treatment led to a gradual reduction for young and mature biofilms, achieving >4-Log reductions after 20 min. Results were also used to develop two predictive inactivation models. Conclusions: This work presents evidence that PAW can be used to inactivate both planktonic cells and biofilms of P. aeruginosa. Experimental and theoretical results also demonstrate that reduction is dependent on discharge frequency and exposure time. Significance and Impact of the Study: This work demonstrates the potential of using PAW as means to control MIC.

AB - Aims: The cost of Microbiologically Influenced Corrosion (MIC) significantly affects a wide range of sectors. This study aims to assess the efficiency of a novel technology based on the use of plasma-activated water (PAW) in inhibiting corrosion caused by bacteria. Methods and Results: This study evaluated the effectiveness of PAW, produced by a plasma bubble reactor, in reducing corrosion causing Pseudomonas aeruginosa planktonic cells in tap water and biofilms were grown onto stainless steel (SS) coupons. Planktonic cells and biofilms were treated with PAW at different discharge frequencies (500–1500 Hz) and exposure times (0–20 min). P. aeruginosa cells in tap water were significantly reduced after treatment, with higher exposure times and discharge frequencies achieving higher reductions. Also, PAW treatment led to a gradual reduction for young and mature biofilms, achieving >4-Log reductions after 20 min. Results were also used to develop two predictive inactivation models. Conclusions: This work presents evidence that PAW can be used to inactivate both planktonic cells and biofilms of P. aeruginosa. Experimental and theoretical results also demonstrate that reduction is dependent on discharge frequency and exposure time. Significance and Impact of the Study: This work demonstrates the potential of using PAW as means to control MIC.

KW - plasma-activated water

KW - biofilm

KW - intracellular ATP levels

KW - P. aeruginosa

KW - microbial corrosion

KW - Biotechnology

KW - Applied Microbiology and Biotechnology

KW - General Medicine

KW - P

KW - aeruginosa

UR - http://www.scopus.com/inward/record.url?scp=85120634666&partnerID=8YFLogxK

U2 - 10.1111/jam.15391

DO - 10.1111/jam.15391

M3 - Article

C2 - 34846774

SN - 1364-5072

VL - 132

SP - 2781

EP - 2794

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

IS - 4

ER -

Inhibition of corrosion causing Pseudomonas aeruginosa using plasma-activated water

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this