Abstract
Objectives: To investigate the influence of key nutrients involved in one-carbon metabolism on DNA methylation in adults.
Data sources: Systematic literature searches were conducted in the Cochrane library, Medline, Embase, CINAHL Plus, Scopus and Web of Science databases. Studies that met the inclusion criteria and were published in English were included.
Data extraction: The first author, study design, sample size, population characteristics, type of intervention and duration, tissue type or cells analyzed, molecular techniques and DNA methylation outcomes.
Data synthesis: A meta-analysis of RCTs was conducted to investigate the effect of one-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization was performed using BioVenn software.
Results: From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicates significant associations between one-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B-12 significantly increased global DNA methylation in studies employing LC-MS, which had markedly lower heterogeneity (n = 3, Z = 3.31, P = 0.0009; I2 = 0%) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to both folate and vitamin B-12 biomarkers.
Conclusions: This study supports significant associations between one-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies.
| Language | English |
|---|---|
| Article number | nuz094 |
| Pages | 1-20 |
| Number of pages | 20 |
| Journal | Nutrition Reviews |
| Early online date | 23 Dec 2019 |
| DOIs | |
| Publication status | Published - 23 Jan 2020 |
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Keywords
- DNA methylation
- B-vitamins
- folate
- epigenetics
- one-carbon metabolism
- nutrients
- adults
- global DNA methylation
Cite this
}
Influence of Nutrients involved in One-Carbon Metabolism on DNA Methylation in Adults : A Systematic Review and Meta-Analysis. / Amenyah, Sophia; Hughes, Catherine; Ward, M; Roxborough, Samuel; Deane, Jennifer; Thursby, Sara-Jayne; Walsh, CP; Kok, D; Strain, Sean; McNulty, H; Lees Murdock, Diane.
In: Nutrition Reviews, 23.01.2020, p. 1-20.Research output: Contribution to journal › Article
TY - JOUR
T1 - Influence of Nutrients involved in One-Carbon Metabolism on DNA Methylation in Adults
T2 - Nutrition Reviews
AU - Amenyah, Sophia
AU - Hughes, Catherine
AU - Ward, M
AU - Roxborough, Samuel
AU - Deane, Jennifer
AU - Thursby, Sara-Jayne
AU - Walsh, CP
AU - Kok, D
AU - Strain, Sean
AU - McNulty, H
AU - Lees Murdock, Diane
PY - 2020/1/23
Y1 - 2020/1/23
N2 - Context: Aberrant DNA methylation is linked to various diseases. The supply of methyl groups for methylation reactions is mediated via S-adenosylmethionine which depends on the availability of folate and related B-vitamins.Objectives: To investigate the influence of key nutrients involved in one-carbon metabolism on DNA methylation in adults.Data sources: Systematic literature searches were conducted in the Cochrane library, Medline, Embase, CINAHL Plus, Scopus and Web of Science databases. Studies that met the inclusion criteria and were published in English were included.Data extraction: The first author, study design, sample size, population characteristics, type of intervention and duration, tissue type or cells analyzed, molecular techniques and DNA methylation outcomes. Data synthesis: A meta-analysis of RCTs was conducted to investigate the effect of one-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization was performed using BioVenn software.Results: From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicates significant associations between one-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B-12 significantly increased global DNA methylation in studies employing LC-MS, which had markedly lower heterogeneity (n = 3, Z = 3.31, P = 0.0009; I2 = 0%) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to both folate and vitamin B-12 biomarkers. Conclusions: This study supports significant associations between one-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies.
AB - Context: Aberrant DNA methylation is linked to various diseases. The supply of methyl groups for methylation reactions is mediated via S-adenosylmethionine which depends on the availability of folate and related B-vitamins.Objectives: To investigate the influence of key nutrients involved in one-carbon metabolism on DNA methylation in adults.Data sources: Systematic literature searches were conducted in the Cochrane library, Medline, Embase, CINAHL Plus, Scopus and Web of Science databases. Studies that met the inclusion criteria and were published in English were included.Data extraction: The first author, study design, sample size, population characteristics, type of intervention and duration, tissue type or cells analyzed, molecular techniques and DNA methylation outcomes. Data synthesis: A meta-analysis of RCTs was conducted to investigate the effect of one-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization was performed using BioVenn software.Results: From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicates significant associations between one-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B-12 significantly increased global DNA methylation in studies employing LC-MS, which had markedly lower heterogeneity (n = 3, Z = 3.31, P = 0.0009; I2 = 0%) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to both folate and vitamin B-12 biomarkers. Conclusions: This study supports significant associations between one-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies.
KW - DNA methylation
KW - B-vitamins
KW - folate
KW - epigenetics
KW - one-carbon metabolism
KW - nutrients
KW - adults
KW - global DNA methylation
U2 - 10.1093/nutrit/nuz094
DO - 10.1093/nutrit/nuz094
M3 - Article
SP - 1
EP - 20
JO - Nutrition Reviews
JF - Nutrition Reviews
SN - 0029-6643
M1 - nuz094
ER -