Influence of Nutrients involved in One-Carbon Metabolism on DNA Methylation in Adults: A Systematic Review and Meta-Analysis

Research output: Contribution to journalArticle

Abstract

Context: Aberrant DNA methylation is linked to various diseases. The supply of methyl groups for methylation reactions is mediated via S-adenosylmethionine which depends on the availability of folate and related B-vitamins.
Objectives: To investigate the influence of key nutrients involved in one-carbon metabolism on DNA methylation in adults.
Data sources: Systematic literature searches were conducted in the Cochrane library, Medline, Embase, CINAHL Plus, Scopus and Web of Science databases. Studies that met the inclusion criteria and were published in English were included.
Data extraction: The first author, study design, sample size, population characteristics, type of intervention and duration, tissue type or cells analyzed, molecular techniques and DNA methylation outcomes.
Data synthesis: A meta-analysis of RCTs was conducted to investigate the effect of one-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization was performed using BioVenn software.
Results: From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicates significant associations between one-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B-12 significantly increased global DNA methylation in studies employing LC-MS, which had markedly lower heterogeneity (n = 3, Z = 3.31, P = 0.0009; I2 = 0%) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to both folate and vitamin B-12 biomarkers.
Conclusions: This study supports significant associations between one-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies.
LanguageEnglish
Article numbernuz094
Pages1-20
Number of pages20
JournalNutrition Reviews
Early online date23 Dec 2019
DOIs
Publication statusPublished - 23 Jan 2020

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DNA Methylation
Meta-Analysis
Carbon
Food
Folic Acid
Vitamin B 12
S-Adenosylmethionine
Vitamin B Complex
Information Storage and Retrieval
Population Characteristics
Sample Size
Methylation
Libraries
Software
Biomarkers
Databases

Keywords

  • DNA methylation
  • B-vitamins
  • folate
  • epigenetics
  • one-carbon metabolism
  • nutrients
  • adults
  • global DNA methylation

Cite this

@article{2a47fb0133df4765ad422684c35cc3b3,
title = "Influence of Nutrients involved in One-Carbon Metabolism on DNA Methylation in Adults: A Systematic Review and Meta-Analysis",
abstract = "Context: Aberrant DNA methylation is linked to various diseases. The supply of methyl groups for methylation reactions is mediated via S-adenosylmethionine which depends on the availability of folate and related B-vitamins.Objectives: To investigate the influence of key nutrients involved in one-carbon metabolism on DNA methylation in adults.Data sources: Systematic literature searches were conducted in the Cochrane library, Medline, Embase, CINAHL Plus, Scopus and Web of Science databases. Studies that met the inclusion criteria and were published in English were included.Data extraction: The first author, study design, sample size, population characteristics, type of intervention and duration, tissue type or cells analyzed, molecular techniques and DNA methylation outcomes. Data synthesis: A meta-analysis of RCTs was conducted to investigate the effect of one-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization was performed using BioVenn software.Results: From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicates significant associations between one-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B-12 significantly increased global DNA methylation in studies employing LC-MS, which had markedly lower heterogeneity (n = 3, Z = 3.31, P = 0.0009; I2 = 0{\%}) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to both folate and vitamin B-12 biomarkers. Conclusions: This study supports significant associations between one-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies.",
keywords = "DNA methylation, B-vitamins, folate, epigenetics, one-carbon metabolism, nutrients, adults, global DNA methylation",
author = "Sophia Amenyah and Catherine Hughes and M Ward and Samuel Roxborough and Jennifer Deane and Sara-Jayne Thursby and CP Walsh and D Kok and Sean Strain and H McNulty and {Lees Murdock}, Diane",
year = "2020",
month = "1",
day = "23",
doi = "10.1093/nutrit/nuz094",
language = "English",
pages = "1--20",
journal = "Nutrition Reviews",
issn = "0029-6643",

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T1 - Influence of Nutrients involved in One-Carbon Metabolism on DNA Methylation in Adults

T2 - Nutrition Reviews

AU - Amenyah, Sophia

AU - Hughes, Catherine

AU - Ward, M

AU - Roxborough, Samuel

AU - Deane, Jennifer

AU - Thursby, Sara-Jayne

AU - Walsh, CP

AU - Kok, D

AU - Strain, Sean

AU - McNulty, H

AU - Lees Murdock, Diane

PY - 2020/1/23

Y1 - 2020/1/23

N2 - Context: Aberrant DNA methylation is linked to various diseases. The supply of methyl groups for methylation reactions is mediated via S-adenosylmethionine which depends on the availability of folate and related B-vitamins.Objectives: To investigate the influence of key nutrients involved in one-carbon metabolism on DNA methylation in adults.Data sources: Systematic literature searches were conducted in the Cochrane library, Medline, Embase, CINAHL Plus, Scopus and Web of Science databases. Studies that met the inclusion criteria and were published in English were included.Data extraction: The first author, study design, sample size, population characteristics, type of intervention and duration, tissue type or cells analyzed, molecular techniques and DNA methylation outcomes. Data synthesis: A meta-analysis of RCTs was conducted to investigate the effect of one-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization was performed using BioVenn software.Results: From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicates significant associations between one-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B-12 significantly increased global DNA methylation in studies employing LC-MS, which had markedly lower heterogeneity (n = 3, Z = 3.31, P = 0.0009; I2 = 0%) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to both folate and vitamin B-12 biomarkers. Conclusions: This study supports significant associations between one-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies.

AB - Context: Aberrant DNA methylation is linked to various diseases. The supply of methyl groups for methylation reactions is mediated via S-adenosylmethionine which depends on the availability of folate and related B-vitamins.Objectives: To investigate the influence of key nutrients involved in one-carbon metabolism on DNA methylation in adults.Data sources: Systematic literature searches were conducted in the Cochrane library, Medline, Embase, CINAHL Plus, Scopus and Web of Science databases. Studies that met the inclusion criteria and were published in English were included.Data extraction: The first author, study design, sample size, population characteristics, type of intervention and duration, tissue type or cells analyzed, molecular techniques and DNA methylation outcomes. Data synthesis: A meta-analysis of RCTs was conducted to investigate the effect of one-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization was performed using BioVenn software.Results: From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicates significant associations between one-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B-12 significantly increased global DNA methylation in studies employing LC-MS, which had markedly lower heterogeneity (n = 3, Z = 3.31, P = 0.0009; I2 = 0%) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to both folate and vitamin B-12 biomarkers. Conclusions: This study supports significant associations between one-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies.

KW - DNA methylation

KW - B-vitamins

KW - folate

KW - epigenetics

KW - one-carbon metabolism

KW - nutrients

KW - adults

KW - global DNA methylation

U2 - 10.1093/nutrit/nuz094

DO - 10.1093/nutrit/nuz094

M3 - Article

SP - 1

EP - 20

JO - Nutrition Reviews

JF - Nutrition Reviews

SN - 0029-6643

M1 - nuz094

ER -