Influence of high-power EM-videopulse on insulated cells

N. I. Gaponenko; N. P. Dikij; Yu F. Lonin; E. P. Medvedeva; N. I. Onishchenko; A. Yu Petrenko; A. I. Tarasov

doi:10.1109/CRMICO.2000.1255849

Influence of high-power EM-videopulse on insulated cells

N. I. Gaponenko, N. P. Dikij, Yu F. Lonin, E. P. Medvedeva, N. I. Onishchenko, A. Yu Petrenko, A. I. Tarasov

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The influence of a short high-power electromagnetic videopulse on a suspension of hepatic cells of 9-weeks human embryo has been investigated. Such pulse was generated by an electron beam of the accelerator "Temp-A" with energy 0.6 MeV, current 4 κA, and time duration 40 nsec falling to rod antenna of 3 m length. The output power was 100 MW. The samples were placed perpendicular to the antenna at 30 cm distance from its center. The free-radical state in the system "hepatocite+hypochlorite" has been investigated using the quantum-metrical set with photo-electronic amplifier FEU-140 in a thermostat box at 37°C. The appearance of radical fragments in this system was registered by means of 630 nm spectral line of generated single oxygen. The estimation of the cell viability before and after irradiation was performed using the method of supravital staining by methylene dark blue. It was shown that the influence of video-pulse treatment decreases the percentage of the viability of the hepatocites and activates the free-radical processes and correspondingly enhances the single oxygen generation. The dependence of these processes on the video-pulse power was determined.

Original language	English
Title of host publication	2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	54-55
Number of pages	2
ISBN (Electronic)	9665720481, 9789665720485
DOIs	https://doi.org/10.1109/CRMICO.2000.1255849
Publication status	Published (in print/issue) - 2000
Event	10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000 - Crimea, Ukraine Duration: 11 Sept 2000 → 15 Sept 2000

Publication series

Name	2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000

Conference

Conference	10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000
Country/Territory	Ukraine
City	Crimea
Period	11/09/00 → 15/09/00

Bibliographical note

Publisher Copyright:
© 2000 CriMiCo.

Access to Document

10.1109/CRMICO.2000.1255849

Cite this

Gaponenko, N. I., Dikij, N. P., Lonin, Y. F., Medvedeva, E. P., Onishchenko, N. I., Petrenko, A. Y., & Tarasov, A. I. (2000). Influence of high-power EM-videopulse on insulated cells. In 2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000 (pp. 54-55). Article 1255849 (2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/CRMICO.2000.1255849

Gaponenko, N. I. ; Dikij, N. P. ; Lonin, Yu F. et al. / Influence of high-power EM-videopulse on insulated cells. 2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000. Institute of Electrical and Electronics Engineers Inc., 2000. pp. 54-55 (2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000).

@inproceedings{e311be3739354af680ee48ea75675506,

title = "Influence of high-power EM-videopulse on insulated cells",

abstract = "The influence of a short high-power electromagnetic videopulse on a suspension of hepatic cells of 9-weeks human embryo has been investigated. Such pulse was generated by an electron beam of the accelerator {"}Temp-A{"} with energy 0.6 MeV, current 4 κA, and time duration 40 nsec falling to rod antenna of 3 m length. The output power was 100 MW. The samples were placed perpendicular to the antenna at 30 cm distance from its center. The free-radical state in the system {"}hepatocite+hypochlorite{"} has been investigated using the quantum-metrical set with photo-electronic amplifier FEU-140 in a thermostat box at 37°C. The appearance of radical fragments in this system was registered by means of 630 nm spectral line of generated single oxygen. The estimation of the cell viability before and after irradiation was performed using the method of supravital staining by methylene dark blue. It was shown that the influence of video-pulse treatment decreases the percentage of the viability of the hepatocites and activates the free-radical processes and correspondingly enhances the single oxygen generation. The dependence of these processes on the video-pulse power was determined.",

author = "Gaponenko, {N. I.} and Dikij, {N. P.} and Lonin, {Yu F.} and Medvedeva, {E. P.} and Onishchenko, {N. I.} and Petrenko, {A. Yu} and Tarasov, {A. I.}",

note = "Publisher Copyright: {\textcopyright} 2000 CriMiCo.; 10th International Crimean Microwave Conference {"}Microwave and Telecommunication Technology{"}, CriMico 2000 ; Conference date: 11-09-2000 Through 15-09-2000",

year = "2000",

doi = "10.1109/CRMICO.2000.1255849",

language = "English",

series = "2000 10th International Crimean Microwave Conference {"}Microwave and Telecommunication Technology{"}, CriMico 2000",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "54--55",

booktitle = "2000 10th International Crimean Microwave Conference {"}Microwave and Telecommunication Technology{"}, CriMico 2000",

address = "United States",

}

Gaponenko, NI, Dikij, NP, Lonin, YF, Medvedeva, EP, Onishchenko, NI, Petrenko, AY & Tarasov, AI 2000, Influence of high-power EM-videopulse on insulated cells. in 2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000., 1255849, 2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000, Institute of Electrical and Electronics Engineers Inc., pp. 54-55, 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000, Crimea, Ukraine, 11/09/00. https://doi.org/10.1109/CRMICO.2000.1255849

Influence of high-power EM-videopulse on insulated cells. / Gaponenko, N. I.; Dikij, N. P.; Lonin, Yu F. et al.
2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000. Institute of Electrical and Electronics Engineers Inc., 2000. p. 54-55 1255849 (2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Influence of high-power EM-videopulse on insulated cells

AU - Gaponenko, N. I.

AU - Dikij, N. P.

AU - Lonin, Yu F.

AU - Medvedeva, E. P.

AU - Onishchenko, N. I.

AU - Petrenko, A. Yu

AU - Tarasov, A. I.

PY - 2000

Y1 - 2000

N2 - The influence of a short high-power electromagnetic videopulse on a suspension of hepatic cells of 9-weeks human embryo has been investigated. Such pulse was generated by an electron beam of the accelerator "Temp-A" with energy 0.6 MeV, current 4 κA, and time duration 40 nsec falling to rod antenna of 3 m length. The output power was 100 MW. The samples were placed perpendicular to the antenna at 30 cm distance from its center. The free-radical state in the system "hepatocite+hypochlorite" has been investigated using the quantum-metrical set with photo-electronic amplifier FEU-140 in a thermostat box at 37°C. The appearance of radical fragments in this system was registered by means of 630 nm spectral line of generated single oxygen. The estimation of the cell viability before and after irradiation was performed using the method of supravital staining by methylene dark blue. It was shown that the influence of video-pulse treatment decreases the percentage of the viability of the hepatocites and activates the free-radical processes and correspondingly enhances the single oxygen generation. The dependence of these processes on the video-pulse power was determined.

AB - The influence of a short high-power electromagnetic videopulse on a suspension of hepatic cells of 9-weeks human embryo has been investigated. Such pulse was generated by an electron beam of the accelerator "Temp-A" with energy 0.6 MeV, current 4 κA, and time duration 40 nsec falling to rod antenna of 3 m length. The output power was 100 MW. The samples were placed perpendicular to the antenna at 30 cm distance from its center. The free-radical state in the system "hepatocite+hypochlorite" has been investigated using the quantum-metrical set with photo-electronic amplifier FEU-140 in a thermostat box at 37°C. The appearance of radical fragments in this system was registered by means of 630 nm spectral line of generated single oxygen. The estimation of the cell viability before and after irradiation was performed using the method of supravital staining by methylene dark blue. It was shown that the influence of video-pulse treatment decreases the percentage of the viability of the hepatocites and activates the free-radical processes and correspondingly enhances the single oxygen generation. The dependence of these processes on the video-pulse power was determined.

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U2 - 10.1109/CRMICO.2000.1255849

DO - 10.1109/CRMICO.2000.1255849

M3 - Conference contribution

AN - SCOPUS:84966389339

T3 - 2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000

SP - 54

EP - 55

BT - 2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000

Y2 - 11 September 2000 through 15 September 2000

ER -

Gaponenko NI, Dikij NP, Lonin YF, Medvedeva EP, Onishchenko NI, Petrenko AY et al. Influence of high-power EM-videopulse on insulated cells. In 2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000. Institute of Electrical and Electronics Engineers Inc. 2000. p. 54-55. 1255849. (2000 10th International Crimean Microwave Conference "Microwave and Telecommunication Technology", CriMico 2000). doi: 10.1109/CRMICO.2000.1255849

Influence of high-power EM-videopulse on insulated cells

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